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Article: Cardiac expression of adiponectin and its receptors in streptozotocin-induced diabetic rats

TitleCardiac expression of adiponectin and its receptors in streptozotocin-induced diabetic rats
Authors
Issue Date2007
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/metabol
Citation
Metabolism: Clinical And Experimental, 2007, v. 56 n. 10, p. 1363-1371 How to Cite?
AbstractAdiponectin can improve both glucose metabolism and insulin resistance via the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Activated AMPK phosphorylates a variety of intracellular proteins, including acetyl coenzyme A carboxylase (ACC) that is involved in fatty acid oxidation. Adenosine monophosphate-activated protein kinase increases glucose transport by stimulating the translocation of glucose transporter 4 (GLUT4) to the sarcolemma in the heart. Adiponectin exerts its effect through adiponectin receptors, which are predominantly expressed in the liver and skeletal muscle. It is unknown whether the cardiac expression of adiponectin and its receptors is changed in diabetic rats. In the present study, we investigated the protein expression of adiponectin and its receptors in streptozotocin (STZ)-induced diabetic rat hearts. We also explored whether the levels of AMPK, ACC, and GLUT4 will be altered with the changed adiponectin and its receptors in STZ diabetic rat hearts. Plasma and cardiac adiponectin levels were measured by radioimmunoassay. Plasma and cardiac interleukin 6 and plasma tumor necrosis factor α (TNF-α) were assayed by enzyme-linked immunosorbent assay. Cardiac adiponectin receptors, AMPK-α, ACC, GLUT4, and TNF-α were analyzed by Western blot in control and STZ diabetic rats. The plasma adiponectin level was decreased, but the cardiac protein expression of adiponectin receptor 1 was increased in diabetic rats. There was no difference in the cardiac adiponectin level and the cardiac adiponectin receptor 2 protein expression between control and diabetic rats. The phosphorylation of AMPK-α and protein expression of GLUT4 were decreased, but the phosphorylation of ACC was unchanged in diabetic rat hearts. Plasma and cardiac levels of interleukin 6 and TNF-α were increased in diabetic rats. In conclusion, STZ-induced diabetes up-regulates adiponectin receptors in the heart. Despite an increase in cardiac adiponectin receptor 1 expression, there is an increased cardiac inflammatory response and a decreased GLUT4 protein expression associated with a reduction in circulating adiponectin. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/147248
ISSN
2015 Impact Factor: 4.375
2015 SCImago Journal Rankings: 1.978
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, Zen_US
dc.contributor.authorXia, Zen_US
dc.contributor.authorYuen, VGen_US
dc.contributor.authorMcneill, JHen_US
dc.date.accessioned2012-05-29T06:01:01Z-
dc.date.available2012-05-29T06:01:01Z-
dc.date.issued2007en_US
dc.identifier.citationMetabolism: Clinical And Experimental, 2007, v. 56 n. 10, p. 1363-1371en_US
dc.identifier.issn0026-0495en_US
dc.identifier.urihttp://hdl.handle.net/10722/147248-
dc.description.abstractAdiponectin can improve both glucose metabolism and insulin resistance via the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Activated AMPK phosphorylates a variety of intracellular proteins, including acetyl coenzyme A carboxylase (ACC) that is involved in fatty acid oxidation. Adenosine monophosphate-activated protein kinase increases glucose transport by stimulating the translocation of glucose transporter 4 (GLUT4) to the sarcolemma in the heart. Adiponectin exerts its effect through adiponectin receptors, which are predominantly expressed in the liver and skeletal muscle. It is unknown whether the cardiac expression of adiponectin and its receptors is changed in diabetic rats. In the present study, we investigated the protein expression of adiponectin and its receptors in streptozotocin (STZ)-induced diabetic rat hearts. We also explored whether the levels of AMPK, ACC, and GLUT4 will be altered with the changed adiponectin and its receptors in STZ diabetic rat hearts. Plasma and cardiac adiponectin levels were measured by radioimmunoassay. Plasma and cardiac interleukin 6 and plasma tumor necrosis factor α (TNF-α) were assayed by enzyme-linked immunosorbent assay. Cardiac adiponectin receptors, AMPK-α, ACC, GLUT4, and TNF-α were analyzed by Western blot in control and STZ diabetic rats. The plasma adiponectin level was decreased, but the cardiac protein expression of adiponectin receptor 1 was increased in diabetic rats. There was no difference in the cardiac adiponectin level and the cardiac adiponectin receptor 2 protein expression between control and diabetic rats. The phosphorylation of AMPK-α and protein expression of GLUT4 were decreased, but the phosphorylation of ACC was unchanged in diabetic rat hearts. Plasma and cardiac levels of interleukin 6 and TNF-α were increased in diabetic rats. In conclusion, STZ-induced diabetes up-regulates adiponectin receptors in the heart. Despite an increase in cardiac adiponectin receptor 1 expression, there is an increased cardiac inflammatory response and a decreased GLUT4 protein expression associated with a reduction in circulating adiponectin. © 2007 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/metabolen_US
dc.relation.ispartofMetabolism: Clinical and Experimentalen_US
dc.subject.meshAmp-Activated Protein Kinasesen_US
dc.subject.meshAcetyl-Coa Carboxylase - Blood - Metabolismen_US
dc.subject.meshAdiponectin - Blood - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshDiabetes Mellitus, Experimental - Metabolismen_US
dc.subject.meshGlucose Transporter Type 4 - Metabolismen_US
dc.subject.meshHeart Function Testsen_US
dc.subject.meshInterleukin-6 - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshMultienzyme Complexes - Metabolismen_US
dc.subject.meshMyocardium - Metabolismen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshProtein-Serine-Threonine Kinases - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshTriglycerides - Blooden_US
dc.subject.meshTumor Necrosis Factor-Alpha - Biosynthesisen_US
dc.titleCardiac expression of adiponectin and its receptors in streptozotocin-induced diabetic ratsen_US
dc.typeArticleen_US
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_US
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.metabol.2007.05.005en_US
dc.identifier.pmid17884446en_US
dc.identifier.scopuseid_2-s2.0-34548603601en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548603601&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume56en_US
dc.identifier.issue10en_US
dc.identifier.spage1363en_US
dc.identifier.epage1371en_US
dc.identifier.isiWOS:000249843100010-
dc.publisher.placeUnited Statesen_US

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