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Article: N-acetylcysteine prevents nitrosative stress-associated depression of blood pressure and heart rate in streptozotocin diabetic rats

TitleN-acetylcysteine prevents nitrosative stress-associated depression of blood pressure and heart rate in streptozotocin diabetic rats
Authors
Issue Date2006
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/
Citation
Journal Of Cardiovascular Pharmacology, 2006, v. 47 n. 4, p. 513-520 How to Cite?
AbstractPrevious studies have indicated that cardiovascular abnormalities such as depressed blood pressure and heart rate occur in streptozotocin (STZ) diabetic rats. Chronic diabetes, which is associated with increased expression of inducible nitric oxide synthase (iNOS) and oxidative stress, may produce peroxynitrite/nitrotyrosine and cause nitrosative stress. We hypothesized that nitrosative stress causes cardiovascular depression in STZ diabetic rats and therefore can be corrected by reducing its formation. Control and STZ diabetic rats were treated orally for 9 weeks with N-acetylcysteine (NAC), an antioxidant and inhibitor of iNOS. At termination, the mean arterial blood pressure (MABP) and heart rate (HR) were measured in conscious rats. Nitrotyrosine and endothelial nitric oxide synthase (eNOS) and iNOS expression were assessed in the heart and mesenteric arteries by immunohistochemistry and Western blot experiments. Untreated diabetic rats showed depressed MABP and HR that was prevented by treatment with NAC. In untreated diabetic rats, levels of 15-F(2t)-isoprostane, an indicator of lipid peroxidation increased, whereas plasma nitric oxide and antioxidant concentrations decreased. Furthermore, decreased eNOS and increased iNOS expression were associated with elevated nitrosative stress in blood vessel and heart tissue of untreated diabetic rats. N-acetylcysteine treatment of diabetic rats not only restored the antioxidant capacity but also reduced the expression of iNOS and nitrotyrosine and normalized the expression of eNOS to that of control rats in heart and superior mesenteric arteries. The results suggest that nitrosative stress depress MABP and HR following diabetes. Further studies are required to elucidate the mechanisms involved in nitrosative stress mediated depression of blood pressure and heart rate. Copyright © 2006 by Lippincott Williams & Wilkins.
Persistent Identifierhttp://hdl.handle.net/10722/147234
ISSN
2015 Impact Factor: 2.462
2015 SCImago Journal Rankings: 0.962
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNagareddy, PRen_US
dc.contributor.authorXia, Zen_US
dc.contributor.authorMacleod, KMen_US
dc.contributor.authorMcneill, JHen_US
dc.date.accessioned2012-05-29T06:00:57Z-
dc.date.available2012-05-29T06:00:57Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Cardiovascular Pharmacology, 2006, v. 47 n. 4, p. 513-520en_US
dc.identifier.issn0160-2446en_US
dc.identifier.urihttp://hdl.handle.net/10722/147234-
dc.description.abstractPrevious studies have indicated that cardiovascular abnormalities such as depressed blood pressure and heart rate occur in streptozotocin (STZ) diabetic rats. Chronic diabetes, which is associated with increased expression of inducible nitric oxide synthase (iNOS) and oxidative stress, may produce peroxynitrite/nitrotyrosine and cause nitrosative stress. We hypothesized that nitrosative stress causes cardiovascular depression in STZ diabetic rats and therefore can be corrected by reducing its formation. Control and STZ diabetic rats were treated orally for 9 weeks with N-acetylcysteine (NAC), an antioxidant and inhibitor of iNOS. At termination, the mean arterial blood pressure (MABP) and heart rate (HR) were measured in conscious rats. Nitrotyrosine and endothelial nitric oxide synthase (eNOS) and iNOS expression were assessed in the heart and mesenteric arteries by immunohistochemistry and Western blot experiments. Untreated diabetic rats showed depressed MABP and HR that was prevented by treatment with NAC. In untreated diabetic rats, levels of 15-F(2t)-isoprostane, an indicator of lipid peroxidation increased, whereas plasma nitric oxide and antioxidant concentrations decreased. Furthermore, decreased eNOS and increased iNOS expression were associated with elevated nitrosative stress in blood vessel and heart tissue of untreated diabetic rats. N-acetylcysteine treatment of diabetic rats not only restored the antioxidant capacity but also reduced the expression of iNOS and nitrotyrosine and normalized the expression of eNOS to that of control rats in heart and superior mesenteric arteries. The results suggest that nitrosative stress depress MABP and HR following diabetes. Further studies are required to elucidate the mechanisms involved in nitrosative stress mediated depression of blood pressure and heart rate. Copyright © 2006 by Lippincott Williams & Wilkins.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/en_US
dc.relation.ispartofJournal of Cardiovascular Pharmacologyen_US
dc.subject.meshAcetylcysteine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood Glucose - Metabolismen_US
dc.subject.meshBlood Pressure - Drug Effectsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCholesterol - Blooden_US
dc.subject.meshDiabetes Mellitus, Experimental - Physiopathologyen_US
dc.subject.meshFree Radical Scavengers - Pharmacologyen_US
dc.subject.meshHeart Rate - Drug Effectsen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInsulin - Blooden_US
dc.subject.meshIsoprostanes - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshNitrates - Blood - Metabolism - Physiologyen_US
dc.subject.meshNitric Oxide Synthase Type Ii - Metabolismen_US
dc.subject.meshNitric Oxide Synthase Type Iii - Metabolismen_US
dc.subject.meshNitrites - Blooden_US
dc.subject.meshOxidative Stress - Physiologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshStress, Physiological - Physiopathologyen_US
dc.subject.meshTriglycerides - Blooden_US
dc.subject.meshTyrosine - Analogs & Derivatives - Biosynthesisen_US
dc.titleN-acetylcysteine prevents nitrosative stress-associated depression of blood pressure and heart rate in streptozotocin diabetic ratsen_US
dc.typeArticleen_US
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_US
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/01.fjc.0000211744.93701.25en_US
dc.identifier.pmid16680064-
dc.identifier.scopuseid_2-s2.0-33748199512en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748199512&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue4en_US
dc.identifier.spage513en_US
dc.identifier.epage520en_US
dc.identifier.isiWOS:000237434900003-
dc.publisher.placeUnited Statesen_US

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