Article: Protective effect of propofol against H 2O 2 potentiated TNF-alpha induced human vascular endothelial cell apoptosis

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TitleProtective effect of propofol against H 2O 2 potentiated TNF-alpha induced human vascular endothelial cell apoptosis
AuthorsWang, F1
Xia, Z1
Luo, T1
Ouyang, J2
Xia, Z
KeywordsApoptosis
Endothelium
Hydrogen Peroxide
Propofol
Tumor Necrosis Factor-Alpha
Issue Date2006
CitationMedical Journal Of Wuhan University, 2006, v. 27 n. 3, p. 338-341 [How to Cite?]
AbstractObjective: To test the hypothesis that oxygen free radicals can potentiate TNF-α cellular toxicity, which might be reserved by propofol in human vascular endothelial cells. Methods: Cultured human ECV304 cells were divided into five groups: untreated (Control), treated with 10 μmol/L hydrogen peroxide (H 2O 2) and treated with 40 nmol/L TNF-α alone (T) or in the presence of H 2O 2 (T+H) or in propofol + H 2O 2 (T+H+P), with each cultured for 24 hours. Cell apoptosis was assessed by flow cytometry. The concentration of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected at the same time. Results: H 2O 2, at 10 μmol/L, did not cause significant lipid peroxidation, but enhanced TNF-α induced cell apoptosis (P<0.01). Propofol at 50 μmol/L attenuated TNF-α and H 2O 2 induced cell apoptosis, accompanied with a decrease in MDA, while with increases in SOD and GSH-Px. Conclusion: H 2O 2, even at trace concentration, may significantly enhance TNF-α induced endothelial cell apoptosis. Propofol exerts protective effects against H 2O 2 potentiated TNF-α cell toxicity by reduction of oxidative injury.
ISSN1671-8852
2011 SCImago Journal Rankings: 0.026
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWang, F
dc.contributor.authorXia, Z
dc.contributor.authorLuo, T
dc.contributor.authorOuyang, J
dc.contributor.authorXia, Z
dc.date.accessioned2012-05-29T06:00:56Z
dc.date.available2012-05-29T06:00:56Z
dc.date.issued2006
dc.description.abstractObjective: To test the hypothesis that oxygen free radicals can potentiate TNF-α cellular toxicity, which might be reserved by propofol in human vascular endothelial cells. Methods: Cultured human ECV304 cells were divided into five groups: untreated (Control), treated with 10 μmol/L hydrogen peroxide (H 2O 2) and treated with 40 nmol/L TNF-α alone (T) or in the presence of H 2O 2 (T+H) or in propofol + H 2O 2 (T+H+P), with each cultured for 24 hours. Cell apoptosis was assessed by flow cytometry. The concentration of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected at the same time. Results: H 2O 2, at 10 μmol/L, did not cause significant lipid peroxidation, but enhanced TNF-α induced cell apoptosis (P<0.01). Propofol at 50 μmol/L attenuated TNF-α and H 2O 2 induced cell apoptosis, accompanied with a decrease in MDA, while with increases in SOD and GSH-Px. Conclusion: H 2O 2, even at trace concentration, may significantly enhance TNF-α induced endothelial cell apoptosis. Propofol exerts protective effects against H 2O 2 potentiated TNF-α cell toxicity by reduction of oxidative injury.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMedical Journal Of Wuhan University, 2006, v. 27 n. 3, p. 338-341 [How to Cite?]
dc.identifier.epage341
dc.identifier.issn1671-8852
2011 SCImago Journal Rankings: 0.026
dc.identifier.issue3
dc.identifier.scopuseid_2-s2.0-33746359947
dc.identifier.spage338
dc.identifier.urihttp://hdl.handle.net/10722/147232
dc.identifier.volume27
dc.languageeng
dc.relation.ispartofMedical Journal of Wuhan University
dc.relation.referencesReferences in Scopus
dc.subjectApoptosis
dc.subjectEndothelium
dc.subjectHydrogen Peroxide
dc.subjectPropofol
dc.subjectTumor Necrosis Factor-Alpha
dc.titleProtective effect of propofol against H 2O 2 potentiated TNF-alpha induced human vascular endothelial cell apoptosis
dc.typeArticle
Author Affiliations
  1. Hubei General Hospital
  2. Wuhan University