Article: Propofol enhances ischemic tolerance of middle-aged rat hearts: Effects on 15-F2t-isoprostane formation and tissue antioxidant capacity

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TitlePropofol enhances ischemic tolerance of middle-aged rat hearts: Effects on 15-F2t-isoprostane formation and tissue antioxidant capacity
AuthorsXia, Z1
Godin, DV1
Ansley, DM1
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org
CitationCardiovascular Research, 2003, v. 59 n. 1, p. 113-121 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0008-6363(03)00351-1
AbstractObjective: Experimental study has shown that myocardial ischemic tolerance is reduced during middle-age. We investigated the effect of propofol on ischemic tolerance of middle-aged rat hearts. Methods: Hearts of young adult (10 weeks old, Y) and middle-aged rats (20 weeks old, M) were assigned to propofol (P-Y, P-M) and control (C-Y, C-M) groups (n=6 each). Hearts were perfused using a Langendorff preparation with Krebs-Henseleit solution (KH) at constant flow rates. We applied propofol (P-Y, P-M) for 10 min at 12 μg/ml before inducing 40 min global ischemia. During ischemia, saline (C-Y, C-M) or propofol (P-Y, P-M) in saline was perfused through the aorta at 60 μl/min. Propofol in KH was perfused at 12 μg/ml for the first 15 min of reperfusion and subsequently reduced to 5 μg/ml in propofol treatment groups. Coronary effluent was assayed for 15-F2t-isoprostane after equilibration, during ischemia (T1) and at 0.5 (T2) and 5 (T3) min of reperfusion. After 90 min of reperfusion (T4), hearts were harvested to assess tissue antioxidant capacity. Results: In P-Y, we observed an increased latency to ischemic-contracture and a significantly reduced contracture after 35 min ischemia compared to control groups. No ischemic contracture was observed in P-M. There were significantly lower 15-F2t-isoprostane levels in P-M and P-Y than in C-M and C-Y at T1. At T4, the recovery of left ventricular developed pressure in P-M was greater than in P-Y (P<0.05); both were greater than in C-M and C-Y. Conclusion: Propofol enhanced ischemic tolerance of middle-aged hearts, primarily by inhibiting lipid peroxidation. © 2003 European Society of Cardiology. Published by Elsevier Science B.V. All rights reserved.
ISSN0008-6363
2011 Impact Factor: 6.064
2011 SCImago Journal Rankings: 0.649
DOIhttp://dx.doi.org/10.1016/S0008-6363(03)00351-1
ISI Accession Number IDWOS:000184221300014
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorXia, Z
dc.contributor.authorGodin, DV
dc.contributor.authorAnsley, DM
dc.date.accessioned2012-05-29T06:00:45Z
dc.date.available2012-05-29T06:00:45Z
dc.date.issued2003
dc.description.abstractObjective: Experimental study has shown that myocardial ischemic tolerance is reduced during middle-age. We investigated the effect of propofol on ischemic tolerance of middle-aged rat hearts. Methods: Hearts of young adult (10 weeks old, Y) and middle-aged rats (20 weeks old, M) were assigned to propofol (P-Y, P-M) and control (C-Y, C-M) groups (n=6 each). Hearts were perfused using a Langendorff preparation with Krebs-Henseleit solution (KH) at constant flow rates. We applied propofol (P-Y, P-M) for 10 min at 12 μg/ml before inducing 40 min global ischemia. During ischemia, saline (C-Y, C-M) or propofol (P-Y, P-M) in saline was perfused through the aorta at 60 μl/min. Propofol in KH was perfused at 12 μg/ml for the first 15 min of reperfusion and subsequently reduced to 5 μg/ml in propofol treatment groups. Coronary effluent was assayed for 15-F2t-isoprostane after equilibration, during ischemia (T1) and at 0.5 (T2) and 5 (T3) min of reperfusion. After 90 min of reperfusion (T4), hearts were harvested to assess tissue antioxidant capacity. Results: In P-Y, we observed an increased latency to ischemic-contracture and a significantly reduced contracture after 35 min ischemia compared to control groups. No ischemic contracture was observed in P-M. There were significantly lower 15-F2t-isoprostane levels in P-M and P-Y than in C-M and C-Y at T1. At T4, the recovery of left ventricular developed pressure in P-M was greater than in P-Y (P<0.05); both were greater than in C-M and C-Y. Conclusion: Propofol enhanced ischemic tolerance of middle-aged hearts, primarily by inhibiting lipid peroxidation. © 2003 European Society of Cardiology. Published by Elsevier Science B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCardiovascular Research, 2003, v. 59 n. 1, p. 113-121 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0008-6363(03)00351-1
dc.identifier.doihttp://dx.doi.org/10.1016/S0008-6363(03)00351-1
dc.identifier.epage121
dc.identifier.isiWOS:000184221300014
dc.identifier.issn0008-6363
2011 Impact Factor: 6.064
2011 SCImago Journal Rankings: 0.649
dc.identifier.issue1
dc.identifier.pmid12829182
dc.identifier.scopuseid_2-s2.0-0038143236
dc.identifier.spage113
dc.identifier.urihttp://hdl.handle.net/10722/147202
dc.identifier.volume59
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org
dc.publisher.placeUnited Kingdom
dc.relation.ispartofCardiovascular Research
dc.relation.referencesReferences in Scopus
dc.subject.meshAging
dc.subject.meshAnimals
dc.subject.meshAntioxidants - Metabolism - Therapeutic Use
dc.subject.meshDisease Susceptibility
dc.subject.meshMale
dc.subject.meshMyocardial Ischemia - Metabolism
dc.subject.meshMyocardial Reperfusion Injury - Prevention & Control
dc.subject.meshMyocardium - Metabolism
dc.subject.meshOxidants - Pharmacology
dc.subject.meshPerfusion
dc.subject.meshPropofol - Metabolism - Therapeutic Use
dc.subject.meshRandom Allocation
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshThiobarbituric Acid Reactive Substances - Analysis
dc.subject.meshTert-Butylhydroperoxide - Pharmacology
dc.titlePropofol enhances ischemic tolerance of middle-aged rat hearts: Effects on 15-F2t-isoprostane formation and tissue antioxidant capacity
dc.typeArticle
Author Affiliations
  1. The University of British Columbia