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Article: Dose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation
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TitleDose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation
 
AuthorsXia, Z1
Godin, DV1
Chang, TKH1
Ansley, DM1
 
Issue Date2003
 
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
 
CitationCanadian Journal Of Physiology And Pharmacology, 2003, v. 81 n. 1, p. 14-21 [How to Cite?]
DOI: http://dx.doi.org/10.1139/y02-170
 
AbstractWe examined the effects of propofol (2,6-diisopropylphenol) on functional recovery and 15-F2t-isoprostane generation during ischemia-reperfusion in Langendorff-perfused rat hearts. Before the induction of 40 min of global ischemia, hearts were perfused (10 min) with propofol at 5 (lo-P) or 12 μg/mL (hi-P) in saline or with saline only (control). During ischemia, saline, lo-P, or hi-P was perfused through the aorta at 60 μL/min. During the first 15 min of reperfusion, propofol (5 or 12 μg/mL) was continued, followed by perfusion with 5 μg/mL propofol for 75 min in both propofol-treated groups. After 90 min of reperfusion (Rep-90), heart tissues were harvested for assessment of antioxidant status. In hi-P, we observed increased latency to and greater reduction of ischemic contracture relative to the lo-P or control groups. 15-F2t-Isoprostane concentrations increased during ischemia and were significantly lower in hi-P and lo-P than in control (P < 0.01). At Rep-90, myocardial functional recovery was greater in both propofol-treated groups relative to control, and it correlated positively with tissue antioxidant capacity preservation. Tissue antioxidant capacity was better preserved in hi-P than in lo-P treatment (P < 0.05). We conclude that oxidant injury occurs during ischemia and reperfusion, and propofol provides dose-dependent protection primarily by enhancing tissue antioxidant capacity and reducing lipid peroxidation.
 
ISSN0008-4212
2013 Impact Factor: 1.546
 
DOIhttp://dx.doi.org/10.1139/y02-170
 
ISI Accession Number IDWOS:000181344200003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXia, Z
 
dc.contributor.authorGodin, DV
 
dc.contributor.authorChang, TKH
 
dc.contributor.authorAnsley, DM
 
dc.date.accessioned2012-05-29T06:00:43Z
 
dc.date.available2012-05-29T06:00:43Z
 
dc.date.issued2003
 
dc.description.abstractWe examined the effects of propofol (2,6-diisopropylphenol) on functional recovery and 15-F2t-isoprostane generation during ischemia-reperfusion in Langendorff-perfused rat hearts. Before the induction of 40 min of global ischemia, hearts were perfused (10 min) with propofol at 5 (lo-P) or 12 μg/mL (hi-P) in saline or with saline only (control). During ischemia, saline, lo-P, or hi-P was perfused through the aorta at 60 μL/min. During the first 15 min of reperfusion, propofol (5 or 12 μg/mL) was continued, followed by perfusion with 5 μg/mL propofol for 75 min in both propofol-treated groups. After 90 min of reperfusion (Rep-90), heart tissues were harvested for assessment of antioxidant status. In hi-P, we observed increased latency to and greater reduction of ischemic contracture relative to the lo-P or control groups. 15-F2t-Isoprostane concentrations increased during ischemia and were significantly lower in hi-P and lo-P than in control (P < 0.01). At Rep-90, myocardial functional recovery was greater in both propofol-treated groups relative to control, and it correlated positively with tissue antioxidant capacity preservation. Tissue antioxidant capacity was better preserved in hi-P than in lo-P treatment (P < 0.05). We conclude that oxidant injury occurs during ischemia and reperfusion, and propofol provides dose-dependent protection primarily by enhancing tissue antioxidant capacity and reducing lipid peroxidation.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCanadian Journal Of Physiology And Pharmacology, 2003, v. 81 n. 1, p. 14-21 [How to Cite?]
DOI: http://dx.doi.org/10.1139/y02-170
 
dc.identifier.doihttp://dx.doi.org/10.1139/y02-170
 
dc.identifier.epage21
 
dc.identifier.isiWOS:000181344200003
 
dc.identifier.issn0008-4212
2013 Impact Factor: 1.546
 
dc.identifier.issue1
 
dc.identifier.pmid12665253
 
dc.identifier.scopuseid_2-s2.0-0037247879
 
dc.identifier.spage14
 
dc.identifier.urihttp://hdl.handle.net/10722/147197
 
dc.identifier.volume81
 
dc.languageeng
 
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
 
dc.publisher.placeCanada
 
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnesthetics, Intravenous - Administration & Dosage - Pharmacology
 
dc.subject.meshAnimals
 
dc.subject.meshAntioxidants - Metabolism
 
dc.subject.meshDinoprost - Analogs & Derivatives
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshF2-Isoprostanes - Biosynthesis
 
dc.subject.meshHeart - Drug Effects - Physiopathology
 
dc.subject.meshLipid Peroxidation
 
dc.subject.meshMale
 
dc.subject.meshMyocardial Ischemia - Metabolism - Physiopathology - Prevention & Control
 
dc.subject.meshMyocardial Reperfusion Injury - Metabolism - Physiopathology - Prevention & Control
 
dc.subject.meshPropofol - Administration & Dosage - Pharmacology
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshVentricular Function, Left - Drug Effects
 
dc.titleDose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation
 
dc.typeArticle
 
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<description.abstract>We examined the effects of propofol (2,6-diisopropylphenol) on functional recovery and 15-F2t-isoprostane generation during ischemia-reperfusion in Langendorff-perfused rat hearts. Before the induction of 40 min of global ischemia, hearts were perfused (10 min) with propofol at 5 (lo-P) or 12 &#956;g/mL (hi-P) in saline or with saline only (control). During ischemia, saline, lo-P, or hi-P was perfused through the aorta at 60 &#956;L/min. During the first 15 min of reperfusion, propofol (5 or 12 &#956;g/mL) was continued, followed by perfusion with 5 &#956;g/mL propofol for 75 min in both propofol-treated groups. After 90 min of reperfusion (Rep-90), heart tissues were harvested for assessment of antioxidant status. In hi-P, we observed increased latency to and greater reduction of ischemic contracture relative to the lo-P or control groups. 15-F2t-Isoprostane concentrations increased during ischemia and were significantly lower in hi-P and lo-P than in control (P &lt; 0.01). At Rep-90, myocardial functional recovery was greater in both propofol-treated groups relative to control, and it correlated positively with tissue antioxidant capacity preservation. Tissue antioxidant capacity was better preserved in hi-P than in lo-P treatment (P &lt; 0.05). We conclude that oxidant injury occurs during ischemia and reperfusion, and propofol provides dose-dependent protection primarily by enhancing tissue antioxidant capacity and reducing lipid peroxidation.</description.abstract>
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<subject.mesh>Anesthetics, Intravenous - Administration &amp; Dosage - Pharmacology</subject.mesh>
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<subject.mesh>Antioxidants - Metabolism</subject.mesh>
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Author Affiliations
  1. The University of British Columbia