Article: Dose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation

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TitleDose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation
AuthorsXia, Z1
Godin, DV1
Chang, TKH1
Ansley, DM1
Issue Date2003
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
CitationCanadian Journal Of Physiology And Pharmacology, 2003, v. 81 n. 1, p. 14-21 [How to Cite?]
DOI: http://dx.doi.org/10.1139/y02-170
AbstractWe examined the effects of propofol (2,6-diisopropylphenol) on functional recovery and 15-F2t-isoprostane generation during ischemia-reperfusion in Langendorff-perfused rat hearts. Before the induction of 40 min of global ischemia, hearts were perfused (10 min) with propofol at 5 (lo-P) or 12 μg/mL (hi-P) in saline or with saline only (control). During ischemia, saline, lo-P, or hi-P was perfused through the aorta at 60 μL/min. During the first 15 min of reperfusion, propofol (5 or 12 μg/mL) was continued, followed by perfusion with 5 μg/mL propofol for 75 min in both propofol-treated groups. After 90 min of reperfusion (Rep-90), heart tissues were harvested for assessment of antioxidant status. In hi-P, we observed increased latency to and greater reduction of ischemic contracture relative to the lo-P or control groups. 15-F2t-Isoprostane concentrations increased during ischemia and were significantly lower in hi-P and lo-P than in control (P < 0.01). At Rep-90, myocardial functional recovery was greater in both propofol-treated groups relative to control, and it correlated positively with tissue antioxidant capacity preservation. Tissue antioxidant capacity was better preserved in hi-P than in lo-P treatment (P < 0.05). We conclude that oxidant injury occurs during ischemia and reperfusion, and propofol provides dose-dependent protection primarily by enhancing tissue antioxidant capacity and reducing lipid peroxidation.
ISSN0008-4212
2011 Impact Factor: 1.953
2011 SCImago Journal Rankings: 0.149
DOIhttp://dx.doi.org/10.1139/y02-170
ISI Accession Number IDWOS:000181344200003
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorXia, Z
dc.contributor.authorGodin, DV
dc.contributor.authorChang, TKH
dc.contributor.authorAnsley, DM
dc.date.accessioned2012-05-29T06:00:43Z
dc.date.available2012-05-29T06:00:43Z
dc.date.issued2003
dc.description.abstractWe examined the effects of propofol (2,6-diisopropylphenol) on functional recovery and 15-F2t-isoprostane generation during ischemia-reperfusion in Langendorff-perfused rat hearts. Before the induction of 40 min of global ischemia, hearts were perfused (10 min) with propofol at 5 (lo-P) or 12 μg/mL (hi-P) in saline or with saline only (control). During ischemia, saline, lo-P, or hi-P was perfused through the aorta at 60 μL/min. During the first 15 min of reperfusion, propofol (5 or 12 μg/mL) was continued, followed by perfusion with 5 μg/mL propofol for 75 min in both propofol-treated groups. After 90 min of reperfusion (Rep-90), heart tissues were harvested for assessment of antioxidant status. In hi-P, we observed increased latency to and greater reduction of ischemic contracture relative to the lo-P or control groups. 15-F2t-Isoprostane concentrations increased during ischemia and were significantly lower in hi-P and lo-P than in control (P < 0.01). At Rep-90, myocardial functional recovery was greater in both propofol-treated groups relative to control, and it correlated positively with tissue antioxidant capacity preservation. Tissue antioxidant capacity was better preserved in hi-P than in lo-P treatment (P < 0.05). We conclude that oxidant injury occurs during ischemia and reperfusion, and propofol provides dose-dependent protection primarily by enhancing tissue antioxidant capacity and reducing lipid peroxidation.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCanadian Journal Of Physiology And Pharmacology, 2003, v. 81 n. 1, p. 14-21 [How to Cite?]
DOI: http://dx.doi.org/10.1139/y02-170
dc.identifier.doihttp://dx.doi.org/10.1139/y02-170
dc.identifier.epage21
dc.identifier.isiWOS:000181344200003
dc.identifier.issn0008-4212
2011 Impact Factor: 1.953
2011 SCImago Journal Rankings: 0.149
dc.identifier.issue1
dc.identifier.pmid12665253
dc.identifier.scopuseid_2-s2.0-0037247879
dc.identifier.spage14
dc.identifier.urihttp://hdl.handle.net/10722/147197
dc.identifier.volume81
dc.languageeng
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
dc.publisher.placeCanada
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
dc.relation.referencesReferences in Scopus
dc.subject.meshAnesthetics, Intravenous - Administration & Dosage - Pharmacology
dc.subject.meshAnimals
dc.subject.meshAntioxidants - Metabolism
dc.subject.meshDinoprost - Analogs & Derivatives
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshF2-Isoprostanes - Biosynthesis
dc.subject.meshHeart - Drug Effects - Physiopathology
dc.subject.meshLipid Peroxidation
dc.subject.meshMale
dc.subject.meshMyocardial Ischemia - Metabolism - Physiopathology - Prevention & Control
dc.subject.meshMyocardial Reperfusion Injury - Metabolism - Physiopathology - Prevention & Control
dc.subject.meshPropofol - Administration & Dosage - Pharmacology
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshVentricular Function, Left - Drug Effects
dc.titleDose-dependent protection of cardiac function by propofol during ischemia and early reperfusion in rats: Effects on 15-F2t-isoprostane formation
dc.typeArticle
Author Affiliations
  1. The University of British Columbia