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Article: Dendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia
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TitleDendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia
 
AuthorsLiu, Y1
Wu, Y1
Lam, KT1
Lee, PPW1
Tu, W1
Lau, YL1 2
 
Keywordsdendritic cells
IFN-γ
influenza virus
T cells
X-linked agammaglobulinemia
 
Issue Date2012
 
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142
 
CitationJournal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10875-011-9639-y
 
AbstractIntroduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s).
 
ISSN0271-9142
2013 Impact Factor: 2.654
 
DOIhttp://dx.doi.org/10.1007/s10875-011-9639-y
 
ISI Accession Number IDWOS:000305982100002
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU768108
HKU 777108M
Area of Excellence program on Influenza
University Grants Committee of the Hong Kong SAR, ChinaAoE/M-12/06
Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SARHK-09-03-05
Funding Information:

We thank the help from Ms. Winnie Wai Sim Lau to recruit the patients. This work was supported in part by the General Research Fund, Research Grants Council of Hong Kong (HKU768108, HKU 777108M, W. T. and Y.L.L), the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (project no. AoE/M-12/06, Y.L.L. and W. T.), and Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SAR (HK-09-03-05).

 
ReferencesReferences in Scopus
 
GrantsControl of Pandemic and Inter-pandemic Influenza
The Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection
 
DC FieldValue
dc.contributor.authorLiu, Y
 
dc.contributor.authorWu, Y
 
dc.contributor.authorLam, KT
 
dc.contributor.authorLee, PPW
 
dc.contributor.authorTu, W
 
dc.contributor.authorLau, YL
 
dc.date.accessioned2012-05-28T08:18:25Z
 
dc.date.available2012-05-28T08:18:25Z
 
dc.date.issued2012
 
dc.description.abstractIntroduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s).
 
dc.description.naturepublished_or_final_version
 
dc.description.otherSpringer Open Choice, 28 May 2012
 
dc.identifier.citationJournal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10875-011-9639-y
 
dc.identifier.citeulike10314649
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10875-011-9639-y
 
dc.identifier.eissn1573-2592
 
dc.identifier.epage429
 
dc.identifier.hkuros200821
 
dc.identifier.isiWOS:000305982100002
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU768108
HKU 777108M
Area of Excellence program on Influenza
University Grants Committee of the Hong Kong SAR, ChinaAoE/M-12/06
Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SARHK-09-03-05
Funding Information:

We thank the help from Ms. Winnie Wai Sim Lau to recruit the patients. This work was supported in part by the General Research Fund, Research Grants Council of Hong Kong (HKU768108, HKU 777108M, W. T. and Y.L.L), the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (project no. AoE/M-12/06, Y.L.L. and W. T.), and Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SAR (HK-09-03-05).

 
dc.identifier.issn0271-9142
2013 Impact Factor: 2.654
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid22289994
 
dc.identifier.scopuseid_2-s2.0-84862812060
 
dc.identifier.spage421
 
dc.identifier.urihttp://hdl.handle.net/10722/147117
 
dc.identifier.volume32
 
dc.languageEng
 
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Clinical Immunology
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.projectThe Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe Author(s)
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectdendritic cells
 
dc.subjectIFN-γ
 
dc.subjectinfluenza virus
 
dc.subjectT cells
 
dc.subjectX-linked agammaglobulinemia
 
dc.titleDendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong