Article: Dendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia
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- Publisher Website: 10.1007/s10875-011-9639-y
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| Title | Dendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Liu, Y1 Wu, Y1 Lam, KT1 Lee, PPW1 Tu, W1 Lau, YL1 2 | ||||||||||
| Keywords | dendritic cells IFN-γ influenza virus T cells X-linked agammaglobulinemia | ||||||||||
| Issue Date | 2012 | ||||||||||
| Publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142 | ||||||||||
| Citation | Journal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429 [How to Cite?] DOI: http://dx.doi.org/10.1007/s10875-011-9639-y | ||||||||||
| Abstract | Introduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s). | ||||||||||
| ISSN | 0271-9142 2011 Impact Factor: 3.077 2011 SCImago Journal Rankings: 0.355 | ||||||||||
| DOI | http://dx.doi.org/10.1007/s10875-011-9639-y | ||||||||||
| ISI Accession Number ID | WOS:000305982100002
Funding Information: We thank the help from Ms. Winnie Wai Sim Lau to recruit the patients. This work was supported in part by the General Research Fund, Research Grants Council of Hong Kong (HKU768108, HKU 777108M, W. T. and Y.L.L), the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (project no. AoE/M-12/06, Y.L.L. and W. T.), and Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SAR (HK-09-03-05). | ||||||||||
| References | References in Scopus | ||||||||||
| Grants | Control of Pandemic and Inter-pandemic Influenza The Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection |
| dc.contributor.author | Liu, Y | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Wu, Y | ||||||||||
| dc.contributor.author | Lam, KT | ||||||||||
| dc.contributor.author | Lee, PPW | ||||||||||
| dc.contributor.author | Tu, W | ||||||||||
| dc.contributor.author | Lau, YL | ||||||||||
| dc.date.accessioned | 2012-05-28T08:18:25Z | ||||||||||
| dc.date.available | 2012-05-28T08:18:25Z | ||||||||||
| dc.date.issued | 2012 | ||||||||||
| dc.description.abstract | Introduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s). | ||||||||||
| dc.description.grant | Control of Pandemic and Inter-pandemic Influenza | ||||||||||
| dc.description.grant | The Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection | ||||||||||
| dc.description.grantcode | 97655 | ||||||||||
| dc.description.grantcode | 98640 | ||||||||||
| dc.description.nature | published_or_final_version | ||||||||||
| dc.description.other | Springer Open Choice, 28 May 2012 | ||||||||||
| dc.identifier.citation | Journal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429 [How to Cite?] DOI: http://dx.doi.org/10.1007/s10875-011-9639-y | ||||||||||
| dc.identifier.citeulike | 10314649 | ||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s10875-011-9639-y | ||||||||||
| dc.identifier.eissn | 1573-2592 | ||||||||||
| dc.identifier.epage | 429 | ||||||||||
| dc.identifier.hkuros | 200821 | ||||||||||
| dc.identifier.isi | WOS:000305982100002
Funding Information: We thank the help from Ms. Winnie Wai Sim Lau to recruit the patients. This work was supported in part by the General Research Fund, Research Grants Council of Hong Kong (HKU768108, HKU 777108M, W. T. and Y.L.L), the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (project no. AoE/M-12/06, Y.L.L. and W. T.), and Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SAR (HK-09-03-05). | ||||||||||
| dc.identifier.issn | 0271-9142 2011 Impact Factor: 3.077 2011 SCImago Journal Rankings: 0.355 | ||||||||||
| dc.identifier.issue | 3 | ||||||||||
| dc.identifier.openurl | | ||||||||||
| dc.identifier.scopus | eid_2-s2.0-84862812060 | ||||||||||
| dc.identifier.spage | 421 | ||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/147117 | ||||||||||
| dc.identifier.volume | 32 | ||||||||||
| dc.language | Eng | ||||||||||
| dc.publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142 | ||||||||||
| dc.publisher.place | United States | ||||||||||
| dc.relation.ispartof | Journal of Clinical Immunology | ||||||||||
| dc.relation.references | References in Scopus | ||||||||||
| dc.rights | The Author(s) | ||||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||||
| dc.subject | dendritic cells | ||||||||||
| dc.subject | IFN-γ | ||||||||||
| dc.subject | influenza virus | ||||||||||
| dc.subject | T cells | ||||||||||
| dc.subject | X-linked agammaglobulinemia | ||||||||||
| dc.title | Dendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia | ||||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The University of Hong Kong