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Article: Dendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia

TitleDendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemia
Authors
Keywordsdendritic cells
IFN-γ
influenza virus
T cells
X-linked agammaglobulinemia
Issue Date2012
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142
Citation
Journal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429 How to Cite?
Abstract
Introduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/147117
ISSN
2013 Impact Factor: 2.654
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong KongHKU768108
HKU 777108M
Area of Excellence program on Influenza
University Grants Committee of the Hong Kong SAR, ChinaAoE/M-12/06
Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SARHK-09-03-05
Funding Information:

We thank the help from Ms. Winnie Wai Sim Lau to recruit the patients. This work was supported in part by the General Research Fund, Research Grants Council of Hong Kong (HKU768108, HKU 777108M, W. T. and Y.L.L), the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (project no. AoE/M-12/06, Y.L.L. and W. T.), and Research Fund for the Control of Infectious Diseases, Diseases of the Food and Health Bureau of the Hong Kong SAR (HK-09-03-05).

References
Grants

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWu, Yen_HK
dc.contributor.authorLam, KTen_HK
dc.contributor.authorLee, PPWen_HK
dc.contributor.authorTu, Wen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2012-05-28T08:18:25Z-
dc.date.available2012-05-28T08:18:25Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Clinical Immunology, 2012, v. 32 n. 3, p. 421-429en_HK
dc.identifier.issn0271-9142en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147117-
dc.description.abstractIntroduction: Influenza virus is a potential cause of severe disease in the immunocompromised. X-linked agammaglobulinemia (XLA) is a primary immunodeficiency characterized by the lack of immunoglobulin, B cells, and plasma cells, secondary to mutation in Bruton's tyrosine kinase (Btk) gene. Btk is expressed in both B and dendritic cells (DC). However, little is known about the immune response of DC and T cells to influenza virus in XLA patients. Methods: The in vitro maturation and antigen presenting function of monocyte-derived immature DC (imDC) from 12 XLA patients and 23 age-matched normal controls in response to influenza virus were examined. Influenza virus-specific CD4 and CD8 T cell responses in the patients and controls were further determined after administration of inactivated trivalent influenza vaccine. Results: imDC from XLA patients had normal maturation based on major histocompatibility complex (MHC)-I, MHC-II, CD83 and CD86 expression, and interferon (IFN)-α and interleukin-12 production upon influenza virus stimulation. They also had a normal capacity to induce allogeneic T cell proliferation in response to influenza virus. TIV was well tolerated in XLA patients. Influenza virus-specific CD4 +IFN-γ + and CD8 + IFN-γ + T cells and HLA-A2/M1 58-66-tetramer + CTLs could be induced by TIV in XLA patients, and the levels and duration of maintaining these virus-specific cells in XLA patients are comparable to that in normal controls. Conclusion: We demonstrated for the first time that XLA patients have fully competent DC and T cell immune responses to influenza virus. TIV is safe and could be an option for providing T cell-mediated protection against influenza virus infection in XLA patients. © 2012 The Author(s).en_HK
dc.languageEngen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0271-9142en_HK
dc.relation.ispartofJournal of Clinical Immunologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectdendritic cellsen_HK
dc.subjectIFN-γen_HK
dc.subjectinfluenza virusen_HK
dc.subjectT cellsen_HK
dc.subjectX-linked agammaglobulinemiaen_HK
dc.titleDendritic and T cell response to influenza is normal in the patients with X-linked agammaglobulinemiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://www.springerlink.com/link-out/?id=2104&code=V7L2137513T45884&MUD=MPen_US
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s10875-011-9639-yen_HK
dc.identifier.pmid22289994-
dc.identifier.scopuseid_2-s2.0-84862812060en_HK
dc.identifier.hkuros200821-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862812060&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue3en_HK
dc.identifier.spage421en_HK
dc.identifier.epage429en_HK
dc.identifier.eissn1573-2592en_US
dc.identifier.isiWOS:000305982100002-
dc.publisher.placeUnited Statesen_HK
dc.description.otherSpringer Open Choice, 28 May 2012en_US
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.relation.projectThe Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection-
dc.identifier.scopusauthoridLiu, Y=25632591500en_HK
dc.identifier.scopusauthoridWu, Y=44761666500en_HK
dc.identifier.scopusauthoridLam, KT=25630903400en_HK
dc.identifier.scopusauthoridLee, PPW=55261365800en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.citeulike10314649-

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