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Article: Preservation effects on C/N ratios and stable isotope signatures: Of freshwater fishes and benthic macroinvertebrates

TitlePreservation effects on C/N ratios and stable isotope signatures: Of freshwater fishes and benthic macroinvertebrates
Authors
Issue Date2012
PublisherAmerican Society of Limnology and Oceanography, Inc. The Journal's web site is located at http://www.aslo.org/lomethods
Citation
Limnology And Oceanography: Methods, 2012, v. 10 FEBRAURY, p. 75-89 How to Cite?
AbstractThis study examined the effects of fluid preservatives on carbon and nitrogen stable isotopes (δ 13C and δ 15N) and C/N ratios of freshwater animals. Brotia hainanensis snails, Caridina cantonensis and Macrobrachium hainanense shrimps, and Pseudogastromyzon myersi, Liniparhomaloptera disparis, and Ctenogobius duospilus fishes were collected from seven Hong Kong streams, so as to incorporate natural variations in isotopic signals among conspecifics. Samples were preserved with 10% formalin, 70% ethanol, or formalin-ethanol solution (fixation in formalin then storage in ethanol). We compared sample molar C/N, δ 13C, and δ 15N with frozen conspecifics after 30, 60, 90, 180, and 360 d. Increases in C/N were evident in formalin-fixed shrimps and fish only, whereas DC/N attributable to ethanol and formalin-ethanol preservation was insignificant in all species. Chemical preservation generally caused δ 13C depletion in fishes and Δδ 13C significantly declined over time in formalin-ethanol-preserved L. disparis. Formalin-induced d13C shifts were observed in shrimps (C. cantonensis: -1.54‰; M. hainanense: -0.80‰) and snails (-0.25‰) and were relatively consistent when preservation was ≤ 60 d. The influence of formalin-ethanol on C/N and δ 13C was smaller than that of formalin for all species and more consistent than ethanol preservation. δ 15N of all species was unaffected (within ±1‰) by chemical preservation. Effects on isotopic signals were more predictable among fishes than shrimps or snails. Corrections of +1.11‰ and +1.24‰ should be applied to δ 13C of fishes preserved with formalin and formalin-ethanol (respectively) during trophic analysis. We recommend using formalin-ethanol for macroinvertebrates to limit isotopic shifts, especially those preserved for > 60 d. © 2012, by the American Society of Limnology and Oceanography, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/147039
ISSN
2015 Impact Factor: 2.008
2015 SCImago Journal Rankings: 1.303
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, DCPen_HK
dc.contributor.authorLeung, KMYen_HK
dc.contributor.authorDudgeon, Den_HK
dc.date.accessioned2012-05-23T05:54:15Z-
dc.date.available2012-05-23T05:54:15Z-
dc.date.issued2012en_HK
dc.identifier.citationLimnology And Oceanography: Methods, 2012, v. 10 FEBRAURY, p. 75-89en_HK
dc.identifier.issn1541-5856en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147039-
dc.description.abstractThis study examined the effects of fluid preservatives on carbon and nitrogen stable isotopes (δ 13C and δ 15N) and C/N ratios of freshwater animals. Brotia hainanensis snails, Caridina cantonensis and Macrobrachium hainanense shrimps, and Pseudogastromyzon myersi, Liniparhomaloptera disparis, and Ctenogobius duospilus fishes were collected from seven Hong Kong streams, so as to incorporate natural variations in isotopic signals among conspecifics. Samples were preserved with 10% formalin, 70% ethanol, or formalin-ethanol solution (fixation in formalin then storage in ethanol). We compared sample molar C/N, δ 13C, and δ 15N with frozen conspecifics after 30, 60, 90, 180, and 360 d. Increases in C/N were evident in formalin-fixed shrimps and fish only, whereas DC/N attributable to ethanol and formalin-ethanol preservation was insignificant in all species. Chemical preservation generally caused δ 13C depletion in fishes and Δδ 13C significantly declined over time in formalin-ethanol-preserved L. disparis. Formalin-induced d13C shifts were observed in shrimps (C. cantonensis: -1.54‰; M. hainanense: -0.80‰) and snails (-0.25‰) and were relatively consistent when preservation was ≤ 60 d. The influence of formalin-ethanol on C/N and δ 13C was smaller than that of formalin for all species and more consistent than ethanol preservation. δ 15N of all species was unaffected (within ±1‰) by chemical preservation. Effects on isotopic signals were more predictable among fishes than shrimps or snails. Corrections of +1.11‰ and +1.24‰ should be applied to δ 13C of fishes preserved with formalin and formalin-ethanol (respectively) during trophic analysis. We recommend using formalin-ethanol for macroinvertebrates to limit isotopic shifts, especially those preserved for > 60 d. © 2012, by the American Society of Limnology and Oceanography, Inc.en_HK
dc.languageengen_US
dc.publisherAmerican Society of Limnology and Oceanography, Inc. The Journal's web site is located at http://www.aslo.org/lomethodsen_HK
dc.relation.ispartofLimnology and Oceanography: Methodsen_HK
dc.titlePreservation effects on C/N ratios and stable isotope signatures: Of freshwater fishes and benthic macroinvertebratesen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_HK
dc.identifier.emailDudgeon, D: ddudgeon@hku.hken_HK
dc.identifier.authorityLeung, KMY=rp00733en_HK
dc.identifier.authorityDudgeon, D=rp00691en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.4319/lom.2012.10.75en_HK
dc.identifier.scopuseid_2-s2.0-84861777361en_HK
dc.identifier.hkuros199555en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861777361&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issueFEBRAURYen_HK
dc.identifier.spage75en_HK
dc.identifier.epage89en_HK
dc.identifier.isiWOS:000302919200001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, DCP=23089080800en_HK
dc.identifier.scopusauthoridLeung, KMY=7401860738en_HK
dc.identifier.scopusauthoridDudgeon, D=7006559840en_HK

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