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Conference Paper: Genetic and functional comparison of primitive CD34+ leukemic cells that reside in the marrow compartment and in circulation

TitleGenetic and functional comparison of primitive CD34+ leukemic cells that reside in the marrow compartment and in circulation
Authors
KeywordsAcute myeloid leukemic
Microenvironment
Homing
Issue Date2008
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/cr/marketing/index.html
Citation
The 2007 Shanghai International Symposium on Stem Cell Research, Shanghai, China, 6-9 November 2007. In Cell Research, 2008, v. 18 suppl. 1s, p. s140 How to Cite?
AbstractAcute myeloid leukemia (AML) is characterized by an abnormal increase in leukemic blast. These blast cells accumulate in the bone marrow (BM), often results in severe cytopenia. Extravasations of these leukemic blast cells out of the BM into the circulation, and sometimes infiltration of other organs have also been noted in some patients. Previous in vitro assays showed that BM leukemic blast cells have a higher surface CXCR-4 expression and displayed a higher response to SDF-1 induced migration as compared to circulating blasts. However, the intrinsic properties of peripheral blood (PB) and BM blast cells and any possible differences between the two populations in terms of homing efficiency and leukemia initiating potential has never been clearly investigated. In the present study, primitive CD34+ cells of paired BM and PB samples from AML patients were collected and the expression of a panel of phenotypic markers, as well as the homing efficiency of each paired samples were assessed by xenotransplantation into NOD/SCID mice. While there was no significant difference in the expression of surface markers such as CD133, CD123, CD117, CD38 and CD44 between paired BM and PB CD34+ leukemic samples, in vivo homing assay showed that PB CD34+ cells home to the marrow compartment more efficiently than BM CD34+ cells (P < 0.05). In addition, we compared the gene expression profiles of CD34+ cells of 5 paired BM and PB leukemic samples using cDNA microarray. Despite of having a highly similar expression profile, a number of genes involving in G-protein signaling, cell cycle, regulation of apoptosis and also the mitogen activated protein kinase (MAPK) signaling pathway have been found to have a decreased expression in PB CD34+ population. Base on these data, we hypothesized that primitive CD34+ leukemic cells that reside within the BM compartment are vigorously subjected to the influence of the microenvironment. Egress of these cells out of the BM is probably an active event and may reflect a change in cellular properties. Also, the loss of the BM microenvironmental effect on circulating CD34+ leukemic cells may lead to further cellular changes.
DescriptionPoster Session 2
Persistent Identifierhttp://hdl.handle.net/10722/147004
ISSN
2015 Impact Factor: 14.812
2015 SCImago Journal Rankings: 4.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMSen_US
dc.contributor.authorChow, HCHen_US
dc.contributor.authorKwong, YLen_US
dc.contributor.authorLiang, RHSen_US
dc.contributor.authorLeung, AYHen_US
dc.date.accessioned2012-05-23T05:52:23Z-
dc.date.available2012-05-23T05:52:23Z-
dc.date.issued2008en_US
dc.identifier.citationThe 2007 Shanghai International Symposium on Stem Cell Research, Shanghai, China, 6-9 November 2007. In Cell Research, 2008, v. 18 suppl. 1s, p. s140en_US
dc.identifier.issn1001-0602-
dc.identifier.urihttp://hdl.handle.net/10722/147004-
dc.descriptionPoster Session 2-
dc.description.abstractAcute myeloid leukemia (AML) is characterized by an abnormal increase in leukemic blast. These blast cells accumulate in the bone marrow (BM), often results in severe cytopenia. Extravasations of these leukemic blast cells out of the BM into the circulation, and sometimes infiltration of other organs have also been noted in some patients. Previous in vitro assays showed that BM leukemic blast cells have a higher surface CXCR-4 expression and displayed a higher response to SDF-1 induced migration as compared to circulating blasts. However, the intrinsic properties of peripheral blood (PB) and BM blast cells and any possible differences between the two populations in terms of homing efficiency and leukemia initiating potential has never been clearly investigated. In the present study, primitive CD34+ cells of paired BM and PB samples from AML patients were collected and the expression of a panel of phenotypic markers, as well as the homing efficiency of each paired samples were assessed by xenotransplantation into NOD/SCID mice. While there was no significant difference in the expression of surface markers such as CD133, CD123, CD117, CD38 and CD44 between paired BM and PB CD34+ leukemic samples, in vivo homing assay showed that PB CD34+ cells home to the marrow compartment more efficiently than BM CD34+ cells (P < 0.05). In addition, we compared the gene expression profiles of CD34+ cells of 5 paired BM and PB leukemic samples using cDNA microarray. Despite of having a highly similar expression profile, a number of genes involving in G-protein signaling, cell cycle, regulation of apoptosis and also the mitogen activated protein kinase (MAPK) signaling pathway have been found to have a decreased expression in PB CD34+ population. Base on these data, we hypothesized that primitive CD34+ leukemic cells that reside within the BM compartment are vigorously subjected to the influence of the microenvironment. Egress of these cells out of the BM is probably an active event and may reflect a change in cellular properties. Also, the loss of the BM microenvironmental effect on circulating CD34+ leukemic cells may lead to further cellular changes.-
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/cr/marketing/index.html-
dc.relation.ispartofCell Researchen_US
dc.subjectAcute myeloid leukemic-
dc.subjectMicroenvironment-
dc.subjectHoming-
dc.titleGenetic and functional comparison of primitive CD34+ leukemic cells that reside in the marrow compartment and in circulationen_US
dc.typeConference_Paperen_US
dc.identifier.emailCheung, AMS: cheungms@hku.hken_US
dc.identifier.emailChow, HCH: chowch@hku.hken_US
dc.identifier.emailKwong, YL: ylkwong@hku.hken_US
dc.identifier.emailLiang, RHS: rliang@hku.hken_US
dc.identifier.emailLeung, AYH: ayhleung@hku.hk-
dc.identifier.authorityCheung, AMS=rp01572en_US
dc.identifier.authorityKwong, YL=rp00358en_US
dc.identifier.authorityLiang, RHS=rp00345en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/cr.2008.230-
dc.identifier.hkuros199396en_US
dc.identifier.volume18-
dc.identifier.issuesuppl. 1s-
dc.identifier.spages140-
dc.identifier.epages140-
dc.identifier.isiWOS:000260451000141-
dc.publisher.placeUnited Kingdom-
dc.description.otherThe 2007 Shanghai International Symposium on Stem Cell Research, Shanghai, China, 6-9 November 2007. In Cell Research, 2008, v. 18 suppl. 1s, p. s140-

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