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Article: Distinct but phenotypically heterogeneous human cell populations produce rapid recovery of platelets and neutrophils after transplantation

TitleDistinct but phenotypically heterogeneous human cell populations produce rapid recovery of platelets and neutrophils after transplantation
Authors
Issue Date2012
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2012, v. 119 n. 15, p. 3431-3439 How to Cite?
AbstractDelayed recovery of mature blood cells poses a serious, expensive, and often life-threatening problem for many stem cell transplantation recipients, particularly if heavily pretreated and serving as their own donor, or having a CB transplantation as the only therapeutic option. Importantly, the different cells required to ensure a rapid, as well as a permanent, hematopoietic recovery in these patients remain poorly defined. We now show that human CB and mobilized peripheral blood (mPB) collections contain cells that produce platelets and neutrophils within 3 weeks after being transplanted into sublethally irradiated NOD/scid-IL-2Rgammac-null mice. The cells responsible for these 2 outputs are similarly distributed between the aldehyde dehydrogenase-positive and -negative subsets of lineage marker-negative CB and mPB cells, but their overall frequencies vary independently in individual samples. In addition, their total numbers can be seen to be much (> 30-fold) lower in a single 'average' CB transplantation compared with a single 'average' mPB transplantation (normalized for a similar weight of the recipient), consistent with the published differential performance in adult patients of these 2 transplantation products. Experimental testing confirmed the clinical relevance of the surrogate xenotransplantation assay for quantifying cells with rapid platelet regenerative activity, underscoring its potential for future applications.
Persistent Identifierhttp://hdl.handle.net/10722/146881
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Stem Cell Network (SCN) of Canada
Terry Fox Foundation
Terry Fox Research Institute
Canadian Institutes of Health Research
Croucher Foundation (Hong Kong)
SCN of Canada
Michael Smith Foundation for Health Research
Fonds de Recherche du Quebec-Sante (FRQS)
Funding Information:

This work was supported by grants from the Stem Cell Network (SCN) of Canada, The Terry Fox Foundation and the Terry Fox Research Institute, and the Canadian Institutes of Health Research. A. M. S. C. held a Fellowship from the Croucher Foundation (Hong Kong) and K. D. held a Co-Op Award from the SCN of Canada. R. R. B. was supported in part by a Scholar Award from the Michael Smith Foundation for Health Research and also holds a Terry Fox Foundation New Investigator Award. D. C. R. was supported in part by the Fonds de Recherche du Quebec-Sante (FRQS).

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMSen_US
dc.contributor.authorLeung, Den_US
dc.contributor.authorRostamirad, Sen_US
dc.contributor.authorDhillon, Ken_US
dc.contributor.authorMiller, PHen_US
dc.contributor.authorDroumeva, Ren_US
dc.contributor.authorBrinkman, RRen_US
dc.contributor.authorHogge, Den_US
dc.contributor.authorRoy, DCen_US
dc.contributor.authorEaves, CJen_US
dc.date.accessioned2012-05-23T05:48:28Z-
dc.date.available2012-05-23T05:48:28Z-
dc.date.issued2012en_US
dc.identifier.citationBlood, 2012, v. 119 n. 15, p. 3431-3439en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/10722/146881-
dc.description.abstractDelayed recovery of mature blood cells poses a serious, expensive, and often life-threatening problem for many stem cell transplantation recipients, particularly if heavily pretreated and serving as their own donor, or having a CB transplantation as the only therapeutic option. Importantly, the different cells required to ensure a rapid, as well as a permanent, hematopoietic recovery in these patients remain poorly defined. We now show that human CB and mobilized peripheral blood (mPB) collections contain cells that produce platelets and neutrophils within 3 weeks after being transplanted into sublethally irradiated NOD/scid-IL-2Rgammac-null mice. The cells responsible for these 2 outputs are similarly distributed between the aldehyde dehydrogenase-positive and -negative subsets of lineage marker-negative CB and mPB cells, but their overall frequencies vary independently in individual samples. In addition, their total numbers can be seen to be much (> 30-fold) lower in a single 'average' CB transplantation compared with a single 'average' mPB transplantation (normalized for a similar weight of the recipient), consistent with the published differential performance in adult patients of these 2 transplantation products. Experimental testing confirmed the clinical relevance of the surrogate xenotransplantation assay for quantifying cells with rapid platelet regenerative activity, underscoring its potential for future applications.-
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_US
dc.relation.ispartofBlooden_US
dc.rightsThis research was originally published in The Hematologist: ASH News and Reports. Author(s). Title. The Hematologist: ASH News and Reports. 2011; Vol 119, Issue 15: pp3431-pp3439. © the American Society of Hematology.en_US
dc.subject.meshBlood Cells - classification - cytology - physiology-
dc.subject.meshBlood Platelets - physiology-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshInfant, Newborn-
dc.subject.meshNeutrophils - physiology-
dc.titleDistinct but phenotypically heterogeneous human cell populations produce rapid recovery of platelets and neutrophils after transplantationen_US
dc.typeArticleen_US
dc.identifier.emailCheung, AMS: cheungms@hku.hken_US
dc.identifier.authorityCheung, AMS=rp01572en_US
dc.description.naturelink_to_OA_fulltext-
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1182/blood-2011-12-398024-
dc.identifier.pmid22374695-
dc.identifier.pmcidPMC3358249-
dc.identifier.scopuseid_2-s2.0-84859853646-
dc.identifier.hkuros199389en_US
dc.identifier.volume119en_US
dc.identifier.issue15en_US
dc.identifier.spage3431en_US
dc.identifier.epage3439en_US
dc.identifier.isiWOS:000302917200015-
dc.publisher.placeUnited Statesen_US

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