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Article: Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma

TitleEffect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
Authors
KeywordsEndothelin-1
Glaucoma
Lycium Barbarum Polysaccharides
Retinal Ganglion Cell
Issue Date2012
PublisherMedknow Publications and Media Pvt. Ltd. The Journal's web site is located at http://www.nrronline.org/
Citation
Neural Regeneration Research, 2012, v. 7 n. 9, p. 645-651 How to Cite?
Abstract
Lycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.
Persistent Identifierhttp://hdl.handle.net/10722/146852
ISSN
2013 Impact Factor: 0.234
2013 SCImago Journal Rankings: 0.158
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorMi, XSen_HK
dc.contributor.authorChiu, Ken_HK
dc.contributor.authorVan, Gen_HK
dc.contributor.authorLeung, JWCen_HK
dc.contributor.authorLo, ACYen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2012-05-23T05:42:33Z-
dc.date.available2012-05-23T05:42:33Z-
dc.date.issued2012en_HK
dc.identifier.citationNeural Regeneration Research, 2012, v. 7 n. 9, p. 645-651en_HK
dc.identifier.issn1673-5374en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146852-
dc.description.abstractLycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.en_HK
dc.languageengen_US
dc.publisherMedknow Publications and Media Pvt. Ltd. The Journal's web site is located at http://www.nrronline.org/en_HK
dc.relation.ispartofNeural Regeneration Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEndothelin-1en_HK
dc.subjectGlaucomaen_HK
dc.subjectLycium Barbarum Polysaccharidesen_HK
dc.subjectRetinal Ganglion Cellen_HK
dc.titleEffect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucomaen_HK
dc.typeArticleen_HK
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hku.hken_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturePublished_or_final_version-
dc.identifier.doi10.3969/j.issn.1673-5374.2012.09.001-
dc.identifier.scopuseid_2-s2.0-84861205105en_HK
dc.identifier.hkuros199710en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861205105&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue9en_HK
dc.identifier.spage645en_HK
dc.identifier.epage651en_HK
dc.identifier.isiWOS:000303778000001-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridMi, XS=55222281900en_HK
dc.identifier.scopusauthoridChiu, K=15076970500en_HK
dc.identifier.scopusauthoridVan, G=55222012800en_HK
dc.identifier.scopusauthoridLeung, JWC=8978634300en_HK
dc.identifier.scopusauthoridLo, ACY=7102780640en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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