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Article: Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
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TitleEffect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
 
AuthorsMi, XS1
Chiu, K1
Van, G1
Leung, JWC1
Lo, ACY1
Chung, SK1
Chang, RCC1
So, KF1 2
 
KeywordsEndothelin-1
Glaucoma
Lycium Barbarum Polysaccharides
Retinal Ganglion Cell
 
Issue Date2012
 
PublisherNeural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
 
CitationNeural Regeneration Research, 2012, v. 7 n. 9, p. 645-651 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.001
 
AbstractLycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.
 
DescriptionFulltext link: http://www.nrronline.org/temp/NeuralRegenRes79645-8315506_230555.pdf
 
ISSN1673-5374
2013 Impact Factor: 0.234
2013 SCImago Journal Rankings: 0.158
 
DOIhttp://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.001
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorMi, XS
 
dc.contributor.authorChiu, K
 
dc.contributor.authorVan, G
 
dc.contributor.authorLeung, JWC
 
dc.contributor.authorLo, ACY
 
dc.contributor.authorChung, SK
 
dc.contributor.authorChang, RCC
 
dc.contributor.authorSo, KF
 
dc.date.accessioned2012-05-23T05:42:33Z
 
dc.date.available2012-05-23T05:42:33Z
 
dc.date.issued2012
 
dc.description.abstractLycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.descriptionFulltext link: http://www.nrronline.org/temp/NeuralRegenRes79645-8315506_230555.pdf
 
dc.identifier.citationNeural Regeneration Research, 2012, v. 7 n. 9, p. 645-651 [How to Cite?]
DOI: http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.001
 
dc.identifier.doihttp://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.001
 
dc.identifier.epage651
 
dc.identifier.hkuros199710
 
dc.identifier.issn1673-5374
2013 Impact Factor: 0.234
2013 SCImago Journal Rankings: 0.158
 
dc.identifier.issue9
 
dc.identifier.scopuseid_2-s2.0-84861205105
 
dc.identifier.spage645
 
dc.identifier.urihttp://hdl.handle.net/10722/146852
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherNeural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
 
dc.publisher.placeChina
 
dc.relation.ispartofNeural Regeneration Research
 
dc.relation.referencesReferences in Scopus
 
dc.subjectEndothelin-1
 
dc.subjectGlaucoma
 
dc.subjectLycium Barbarum Polysaccharides
 
dc.subjectRetinal Ganglion Cell
 
dc.titleEffect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
 
dc.typeArticle
 
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<contributor.author>Lo, ACY</contributor.author>
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<description.abstract>Lycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong