Article: Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma

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Title	Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
Authors	Mi, XS1 Chiu, K1 Van, G1 Leung, JWC1 Lo, ACY1 Chung, SK1 Chang, RCC1 So, KF1 2
Keywords	Endothelin-1 Glaucoma Lycium Barbarum Polysaccharides Retinal Ganglion Cell
Issue Date	2012
Publisher	Neural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
Citation	Neural Regeneration Research, 2012, v. 7 n. 9, p. 645-651 [How to Cite?]
Abstract	Lycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.
ISSN	1673-5374 2011 Impact Factor: 0.216 2011 SCImago Journal Rankings: 0.029
References	References in Scopus

DC Field	Value
dc.contributor.author	Mi, XS
dc.contributor.author	Chiu, K
dc.contributor.author	Van, G
dc.contributor.author	Leung, JWC
dc.contributor.author	Lo, ACY
dc.contributor.author	Chung, SK
dc.contributor.author	Chang, RCC
dc.contributor.author	So, KF
dc.date.accessioned	2012-05-23T05:42:33Z
dc.date.available	2012-05-23T05:42:33Z
dc.date.issued	2012
dc.description.abstract	Lycium barbarum, a traditional Chinese anti-aging herb, has been shown to protect retinal ganglion cells (RGCs) in a rat chronic ocular hypertension (COH) model. Here, we investigated the expression of endothelin-1 (ET-1), a strong vasoconstrictor, and its receptors, ET A and ET B, in the COH model and assessed the effects of Lycium barbarum on the ET-1 axis. Elevated intraocular pressure (IOP) was induced in the right eye of SD rats using argon laser photocoagulation. (1) The expression of ET-1, ET A and ET B in normal and COH retinas was studied. (2) Some COH rats were fed daily with Lycium barbarum Polysaccharides (LBP) using 1 mg/kg or phosphate-buffered saline (PBS) for 3 weeks (started 1 week before photocoagulation). The effects of LBP on the expression of ET-1 and its receptors, ET A and ET B, in COH retina were evaluated. A semi-quantitative analysis of staining intensity was used to evaluate the expression levels of ET-1, ET A and ET B in retinal vasculature. We found that (1) Under COH condition, the immunoreactivity of ET-1 was increased in retina associated with an increase of ET B receptor immunoreactivity and a decrease of ET A receptor immunoreactivity. (2) After feeding COH rats with LBP, the expression of ET-1 was decreased with an increase of ET A expression and a decrease of ET B expression in the retina, especially in RGCs. (3) By comparing the staining intensity in the vasculature of COH retina in LBP-fed group with PBS-fed group, there was a decrease in the expression of ET-1 and ET A and an increase in ET B. In summary, ET-1 expression was up-regulated in the retina in COH model. LBP could decrease the expression of ET-1 and modulate the expression of its receptors, ET A and ET B, under the condition of COH. The neuroprotective effect of LBP on RGCs might be related to its ability to regulate the ET-1-mediated biological effects on RGCs and retinal vasculature.
dc.description.nature	Link_to_subscribed_fulltext
dc.identifier.citation	Neural Regeneration Research, 2012, v. 7 n. 9, p. 645-651 [How to Cite?]
dc.identifier.epage	651
dc.identifier.hkuros	199710
dc.identifier.issn	1673-5374 2011 Impact Factor: 0.216 2011 SCImago Journal Rankings: 0.029
dc.identifier.issue	9
dc.identifier.scopus	eid_2-s2.0-84861205105
dc.identifier.spage	645
dc.identifier.uri	http://hdl.handle.net/10722/146852
dc.identifier.volume	7
dc.language	eng
dc.publisher	Neural Regeneration Research. The Journal's web site is located at http://www.sjzsyj.com
dc.publisher.place	China
dc.relation.ispartof	Neural Regeneration Research
dc.relation.references	References in Scopus
dc.subject	Endothelin-1
dc.subject	Glaucoma
dc.subject	Lycium Barbarum Polysaccharides
dc.subject	Retinal Ganglion Cell
dc.title	Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma
dc.type	Article

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Author Affiliations

The University of Hong Kong Li Ka Shing Faculty of Medicine
The University of Hong Kong

Article: Effect of Lycium barbarum polysaccharides on the expression of endothelin-1 and its receptors in an ocular hypertension model of rat glaucoma

The HKU Scholars Hub has contact details for these author(s).