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Article: Lycium barbarum polysaccharides protect mice liver from carbon tetrachloride-induced oxidative stress and necroinflammation

TitleLycium barbarum polysaccharides protect mice liver from carbon tetrachloride-induced oxidative stress and necroinflammation
Authors
KeywordsCarbon tetrachloride
Inflammation
Liver
Lycium barbarum polysaccharides
Nuclear factor kappa-B
Oxidative stress
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 462-470 How to Cite?
AbstractEthnopharmacological relevance: Lycium barbarum has been used as a traditional Chinese medicine to nourish liver, kidneys and the eyes. Aim of the study: We investigated the protective mechanisms of Wolfberry, Lycium barbarum polysaccharides (LBP) in carbon tetrachloride (CCl 4)-induced acute liver injury. Materials and methods: Mice were intraperitoneally injected with a 50 μl/kg CCl 4 to induce acute hepatotoxicity (8 h) and were orally fed with LBP 2 h before the CCl 4 injection. There were six experimental groups of mice (n = 7-8 per group), namely: control mice (vehicle only; 1 mg/kg LBP or 10 mg/kg LBP), CCl 4-treated mice and CCl 4 + LBP treated mice (1 mg/kg LBP or 10 mg/kg LBP). Results: Pre-treatment with LBP effectively reduced the hepatic necrosis and the serum ALT level induced by CCl 4 intoxication. LBP remarkably inhibited cytochrome P450 2E1 expression and restored the expression levels of antioxidant enzymes. It also decreased the level of nitric oxide metabolism and lipid peroxidation induced by CCl 4. LBP attenuated hepatic inflammation via down-regulation of proinflammatory mediators and chemokines. Furthermore, LBP promoted liver regeneration after CCl 4 treatment. The protective effects of LBP against hepatotoxicity were partly through the down-regulation of nuclear factor kappa-B activity. Conclusion: LBP is effective in reducing necroinflammation and oxidative stress induced by a chemical toxin, thus it has a great potential use as a food supplement in the prevention of hepatic diseases. © 2011 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/146850
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
Funding AgencyGrant Number
University Research Committee, HKU
University Grant Council, Hong Kong SAR
Azalea
Funding Information:

We would like to thank Ms. Carman Leung for her technical help in this project. This study was supported by Seed Funding for Basic Research, University Research Committee, HKU, General Research Fund, University Grant Council, Hong Kong SAR and the Azalea (1972) Endowment Fund to KFS and RCCC.

References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Jen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorTipoe, GLen_HK
dc.date.accessioned2012-05-23T05:42:32Z-
dc.date.available2012-05-23T05:42:32Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 462-470en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146850-
dc.description.abstractEthnopharmacological relevance: Lycium barbarum has been used as a traditional Chinese medicine to nourish liver, kidneys and the eyes. Aim of the study: We investigated the protective mechanisms of Wolfberry, Lycium barbarum polysaccharides (LBP) in carbon tetrachloride (CCl 4)-induced acute liver injury. Materials and methods: Mice were intraperitoneally injected with a 50 μl/kg CCl 4 to induce acute hepatotoxicity (8 h) and were orally fed with LBP 2 h before the CCl 4 injection. There were six experimental groups of mice (n = 7-8 per group), namely: control mice (vehicle only; 1 mg/kg LBP or 10 mg/kg LBP), CCl 4-treated mice and CCl 4 + LBP treated mice (1 mg/kg LBP or 10 mg/kg LBP). Results: Pre-treatment with LBP effectively reduced the hepatic necrosis and the serum ALT level induced by CCl 4 intoxication. LBP remarkably inhibited cytochrome P450 2E1 expression and restored the expression levels of antioxidant enzymes. It also decreased the level of nitric oxide metabolism and lipid peroxidation induced by CCl 4. LBP attenuated hepatic inflammation via down-regulation of proinflammatory mediators and chemokines. Furthermore, LBP promoted liver regeneration after CCl 4 treatment. The protective effects of LBP against hepatotoxicity were partly through the down-regulation of nuclear factor kappa-B activity. Conclusion: LBP is effective in reducing necroinflammation and oxidative stress induced by a chemical toxin, thus it has a great potential use as a food supplement in the prevention of hepatic diseases. © 2011 Elsevier Ireland Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectCarbon tetrachlorideen_HK
dc.subjectInflammationen_HK
dc.subjectLiveren_HK
dc.subjectLycium barbarum polysaccharidesen_HK
dc.subjectNuclear factor kappa-Ben_HK
dc.subjectOxidative stressen_HK
dc.subject.meshAlanine Transaminase - blooden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnti-Inflammatory Agents - isolation & purification - pharmacologyen_HK
dc.subject.meshAntioxidants - isolation & purification - pharmacologyen_HK
dc.subject.meshCarbon Tetrachlorideen_HK
dc.subject.meshCytochrome P-450 CYP2E1 - antagonists & inhibitors - metabolismen_HK
dc.subject.meshCytokines - genetics - metabolismen_HK
dc.subject.meshCytoprotectionen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshDrug-Induced Liver Injury - etiology - genetics - metabolism - pathology - prevention & controlen_HK
dc.subject.meshDrugs, Chinese Herbal - isolation & purification - pharmacologyen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Enzymologic - drug effectsen_HK
dc.subject.meshInflammation Mediators - metabolismen_HK
dc.subject.meshLipid Peroxidation - drug effectsen_HK
dc.subject.meshLiver - drug effects - metabolism - pathologyen_HK
dc.subject.meshLycium - chemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshNF-kappa B - metabolismen_HK
dc.subject.meshNecrosisen_HK
dc.subject.meshNitric Oxide - metabolismen_HK
dc.subject.meshOxidative Stress - drug effectsen_HK
dc.subject.meshPlants, Medicinalen_HK
dc.titleLycium barbarum polysaccharides protect mice liver from carbon tetrachloride-induced oxidative stress and necroinflammationen_HK
dc.typeArticleen_HK
dc.identifier.emailChing, YP: ypching@hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2011.11.033en_HK
dc.identifier.pmid22138659-
dc.identifier.scopuseid_2-s2.0-84856016199en_HK
dc.identifier.hkuros199706en_US
dc.identifier.hkuros209836-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84856016199&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume139en_HK
dc.identifier.issue2en_HK
dc.identifier.spage462en_HK
dc.identifier.epage470en_HK
dc.identifier.isiWOS:000302505900020-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridXiao, J=40462781100en_HK
dc.identifier.scopusauthoridLiong, EC=6602732210en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.citeulike10097020-

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