File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Morphometric analyses of retinal sections

TitleMorphometric analyses of retinal sections
Authors
KeywordsCell size
Issue 60
Morphometric analysis
Neuroscience
Retina
Stereo investigator
Thickness
Issue Date2012
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecom
Citation
Journal Of Visualized Experiments, 2012 n. 60, article no. e3377 How to Cite?
AbstractMorphometric analyses of retinal sections have been used in examining retinal diseases. For examples, neuronal cells were significantly lost in the retinal ganglion cell layer (RGCL) in rat models with N-methyl-D-aspartate (NMDA)-induced excitotoxicity, retinal ischemia-reperfusion injury and glaucoma. Reduction of INL and inner plexiform layer (IPL) thicknesses were reversed with citicoline treatment in rats' eyes subjected to kainic acid-mediated glutamate excitotoxicity. Alteration of RGC density and soma sizes were observed with different drug treatments in eyes with elevated intraocular pressure. Therefore, having objective methods of analyzing the retinal morphometries may be of great significance in evaluating retinal pathologies and the effectiveness of therapeutic strategies. The retinal structure is multi-layers and several different kinds of neurons exist in the retina. The morphometric parameters of retina such as cell number, cell size and thickness of different layers are more complex than the cell culture system. Early on, these parameters can be detected using other commercial imaging software. The values are normally of relative value, and changing to the precise value may need further accurate calculation. Also, the tracing of the cell size and morphology may not be accurate and sensitive enough for statistic analysis, especially in the chronic glaucoma model. The measurements used in this protocol provided a more precise and easy way. And the absolute length of the line and size of the cell can be reported directly and easy to be copied to other files. For example, we traced the margin of the inner and outer most nuclei in the INL and formed a line then using the software to draw a 90 degree angle to measure the thickness. While without the help of the software, the line maybe oblique and the changing of retinal thickness may not be repeatable among individual observers. In addition, the number and density of RGCs can also be quantified. This protocol successfully decreases the variability in quantitating features of the retina, increases the sensitivity in detecting minimal changes. This video will demonstrate three types of morphometric analyses of the retinal sections. They include measuring the INL thickness, quantifying the number of RGCs and measuring the sizes of RGCs in absolute value. These three analyses are carried out with Stereo Investigator (MBF Bioscience MicroBrightField, Inc.). The technique can offer a simple but scientific platform for morphometric analyses. © 2012 Journal of Visualized Experiments.
Persistent Identifierhttp://hdl.handle.net/10722/146847
ISSN
2014 Impact Factor: 1.325
2014 SCImago Journal Rankings: 0.424
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, TFen_HK
dc.contributor.authorChiu, Ken_HK
dc.contributor.authorLok, CKMen_HK
dc.contributor.authorHo, WLen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2012-05-23T05:42:31Z-
dc.date.available2012-05-23T05:42:31Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Visualized Experiments, 2012 n. 60, article no. e3377en_HK
dc.identifier.issn1940-087Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/146847-
dc.description.abstractMorphometric analyses of retinal sections have been used in examining retinal diseases. For examples, neuronal cells were significantly lost in the retinal ganglion cell layer (RGCL) in rat models with N-methyl-D-aspartate (NMDA)-induced excitotoxicity, retinal ischemia-reperfusion injury and glaucoma. Reduction of INL and inner plexiform layer (IPL) thicknesses were reversed with citicoline treatment in rats' eyes subjected to kainic acid-mediated glutamate excitotoxicity. Alteration of RGC density and soma sizes were observed with different drug treatments in eyes with elevated intraocular pressure. Therefore, having objective methods of analyzing the retinal morphometries may be of great significance in evaluating retinal pathologies and the effectiveness of therapeutic strategies. The retinal structure is multi-layers and several different kinds of neurons exist in the retina. The morphometric parameters of retina such as cell number, cell size and thickness of different layers are more complex than the cell culture system. Early on, these parameters can be detected using other commercial imaging software. The values are normally of relative value, and changing to the precise value may need further accurate calculation. Also, the tracing of the cell size and morphology may not be accurate and sensitive enough for statistic analysis, especially in the chronic glaucoma model. The measurements used in this protocol provided a more precise and easy way. And the absolute length of the line and size of the cell can be reported directly and easy to be copied to other files. For example, we traced the margin of the inner and outer most nuclei in the INL and formed a line then using the software to draw a 90 degree angle to measure the thickness. While without the help of the software, the line maybe oblique and the changing of retinal thickness may not be repeatable among individual observers. In addition, the number and density of RGCs can also be quantified. This protocol successfully decreases the variability in quantitating features of the retina, increases the sensitivity in detecting minimal changes. This video will demonstrate three types of morphometric analyses of the retinal sections. They include measuring the INL thickness, quantifying the number of RGCs and measuring the sizes of RGCs in absolute value. These three analyses are carried out with Stereo Investigator (MBF Bioscience MicroBrightField, Inc.). The technique can offer a simple but scientific platform for morphometric analyses. © 2012 Journal of Visualized Experiments.en_HK
dc.languageengen_US
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://wwwjovecomen_HK
dc.relation.ispartofJournal of Visualized Experimentsen_HK
dc.subjectCell sizeen_HK
dc.subjectIssue 60en_HK
dc.subjectMorphometric analysisen_HK
dc.subjectNeuroscienceen_HK
dc.subjectRetinaen_HK
dc.subjectStereo investigatoren_HK
dc.subjectThicknessen_HK
dc.titleMorphometric analyses of retinal sectionsen_HK
dc.typeArticleen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3791/3377en_HK
dc.identifier.pmid22370760-
dc.identifier.pmcidPMC3399493-
dc.identifier.scopuseid_2-s2.0-84857817746en_HK
dc.identifier.hkuros199686en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857817746&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_US
dc.identifier.issue60, article no. e3377en_HK
dc.identifier.spagepii 3377 doi: 10.3791/3377en_US
dc.identifier.epagepii 3377 doi: 10.3791/3377en_US
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, TF=55063107500en_HK
dc.identifier.scopusauthoridChiu, K=15076970500en_HK
dc.identifier.scopusauthoridLok, CKM=55065292200en_HK
dc.identifier.scopusauthoridHo, WL=8612000300en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats