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Article: Disinfection effects of MTAD on apical isthmus of the root canal system: a scanning electron microscopic evaluation

TitleDisinfection effects of MTAD on apical isthmus of the root canal system: a scanning electron microscopic evaluation
掃描電鏡評價MTAD對根尖區管間峽部感染物的清除效果
Authors
KeywordsPeriapical periodontitis
Root canal irrigants
Enterococcus faecalis
Sodium hypochlorite
Issue Date2012
PublisherPeking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/
Citation
北京大學學報(醫學版), 2012, v. 44 n. 1, p. 34-38 How to Cite?
Journal of Peking University (Health Sciences), 2012, v. 44 n. 1, p. 34-38 How to Cite?
AbstractOBJECTIVE: To evaluate the disinfection efficacy of MTAD on Enterococcus faecalis biofilm and smear layer colonization in apical isthums of the root canal system. METHODS: Fifteen extracted human maxillary first premolars with isthmus anatomic structure which confirmed by stereo-microscope were contaminated with E. faecalis in vitro and randomly divided into 5 groups: the first group was not treated serving as a baseline control, the second group was treated by normal saline (NS) serving as negative control, the third group was treated by MTAD , the forth group by 5.25% NaOCl, and the fifth group by 5.25% NaOCl + EDTA. All roots in the latter four groups were instrumented by Protaper rotary files and irrigated with respective irrigant, then the roots were split longitudinally and a scanning electron microscope was used to evaluate the antibacterial activity and smear layer cleaning ability of irrigants on isthmus. RESULTS: In the first group, E. faecalis colonized on the isthmus surface and aggregated together to form biofilm-like microorganism community, some bacteria also colonized in the dentinal tubules. When treated with NS, both smear layer and bacteria remained (median of smear layer score was 5). MTAD can remove partial smear layer, and have limited antibacterial activity, some bacteria embedded in smear layer (the median was 3) and were destroyed; In 5.25% NaOCl treatmentgroup, the smear layer was not removed (median of smear layer score was also 5), but all bacteria on the surface were extinguished. The combined use of 5.25% NaOCl and EDTA produced a cleaner isthmus surface and had marked antimicrobial effect, with the median of smear layer score being only 1. CONCLUSION: MTAD may permeate into the isthmus area of apical root canal system, but only performed a partial effect of disinfection and limited antibacterial activity. Sodium hypochlorite cooperated with EDTA can remove infection effectively in the isthmus area.
Persistent Identifierhttp://hdl.handle.net/10722/146836
ISSN
2015 SCImago Journal Rankings: 0.144

 

DC FieldValueLanguage
dc.contributor.authorBao, XDen_US
dc.contributor.authorYao, Nen_US
dc.contributor.authorGao, HJen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorYue, L-
dc.date.accessioned2012-05-23T05:28:08Z-
dc.date.available2012-05-23T05:28:08Z-
dc.date.issued2012en_US
dc.identifier.citation北京大學學報(醫學版), 2012, v. 44 n. 1, p. 34-38en_US
dc.identifier.citationJournal of Peking University (Health Sciences), 2012, v. 44 n. 1, p. 34-38-
dc.identifier.issn1671-167X-
dc.identifier.urihttp://hdl.handle.net/10722/146836-
dc.description.abstractOBJECTIVE: To evaluate the disinfection efficacy of MTAD on Enterococcus faecalis biofilm and smear layer colonization in apical isthums of the root canal system. METHODS: Fifteen extracted human maxillary first premolars with isthmus anatomic structure which confirmed by stereo-microscope were contaminated with E. faecalis in vitro and randomly divided into 5 groups: the first group was not treated serving as a baseline control, the second group was treated by normal saline (NS) serving as negative control, the third group was treated by MTAD , the forth group by 5.25% NaOCl, and the fifth group by 5.25% NaOCl + EDTA. All roots in the latter four groups were instrumented by Protaper rotary files and irrigated with respective irrigant, then the roots were split longitudinally and a scanning electron microscope was used to evaluate the antibacterial activity and smear layer cleaning ability of irrigants on isthmus. RESULTS: In the first group, E. faecalis colonized on the isthmus surface and aggregated together to form biofilm-like microorganism community, some bacteria also colonized in the dentinal tubules. When treated with NS, both smear layer and bacteria remained (median of smear layer score was 5). MTAD can remove partial smear layer, and have limited antibacterial activity, some bacteria embedded in smear layer (the median was 3) and were destroyed; In 5.25% NaOCl treatmentgroup, the smear layer was not removed (median of smear layer score was also 5), but all bacteria on the surface were extinguished. The combined use of 5.25% NaOCl and EDTA produced a cleaner isthmus surface and had marked antimicrobial effect, with the median of smear layer score being only 1. CONCLUSION: MTAD may permeate into the isthmus area of apical root canal system, but only performed a partial effect of disinfection and limited antibacterial activity. Sodium hypochlorite cooperated with EDTA can remove infection effectively in the isthmus area.-
dc.languagechien_US
dc.publisherPeking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/-
dc.relation.ispartof北京大學學報(醫學版)en_US
dc.relation.ispartofJournal of Peking University (Health Sciences)-
dc.subjectPeriapical periodontitis-
dc.subjectRoot canal irrigants-
dc.subjectEnterococcus faecalis-
dc.subjectSodium hypochlorite-
dc.titleDisinfection effects of MTAD on apical isthmus of the root canal system: a scanning electron microscopic evaluationen_US
dc.title掃描電鏡評價MTAD對根尖區管間峽部感染物的清除效果-
dc.typeArticleen_US
dc.identifier.emailZhang, C: zhangcf@hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3969/j.issn.1671-167X.2012.01.007-
dc.identifier.pmid22353896-
dc.identifier.hkuros199780en_US
dc.identifier.volume44en_US
dc.identifier.issue1en_US
dc.identifier.spage34en_US
dc.identifier.epage38en_US
dc.publisher.placeChina-

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