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Article: Coculture of dental pulp stem cells with endothelial cells enhances osteo-/odontogenic and angiogenic potential in vitro

TitleCoculture of dental pulp stem cells with endothelial cells enhances osteo-/odontogenic and angiogenic potential in vitro
Authors
KeywordsDental pulp stem cells
endothelial cells
regenerative endodontics
synergism
Issue Date2012
PublisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com
Citation
Journal Of Endodontics, 2012, v. 38 n. 4, p. 454-463 How to Cite?
AbstractIntroduction: Dental pulp stem cells (DPSCs) have received much attention as a promising population of stem cells in regenerative endodontics. Securing a good blood supply during regeneration is a challenging task because of the constricted apical canal opening, which allows only a limited blood supply. The aim of this study was to investigate any potential synergistic effects of dental pulp stem cells and endothelial cells (ECs) on osteo-/odontogenic and angiogenic differentiation in vitro. Methods: Different ratios of DPSCs and ECs were cultured in direct contact using optimized medium for coculture. The 70% confluent cocultures were incubated in the osteo-/odontogenic differentiation medium for up to 3 weeks. Alkaline phosphatase (ALP) activity, the expression levels of ALP, bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP) genes, and alizarin red staining for mineralization at different time points were analyzed. The tubular network formation on Matrigel and the gene expression levels of CD117, VEGF, CD34, and Flk-1 were used as assays to analyze angiogenesis. Results: The quantification of ALP in DPSC:EC cocultures revealed a greater ALP activity compared with DPSC-alone cultures. At all the time points, 1:1 cultures showed a significantly greater ALP activity than that of DPSC-alone cultures. Alizarin red staining and quantification revealed a much greater amount of calcification in the 1:1 and 1:5 cocultures compared with other cultures (P <.01). The expression levels of ALP, BSP, and DSPP genes further confirmed the greater osteo-/odontogenic differentiation in cocultures compared with those of DPSC-alone cultures. Matrigel assay showed that the addition of DPSCs stabilized preexisting vessel-like structures formed by ECs and increased the longevity of them. Conclusions: Direct coculture of DPSCs and ECs enhances the in vitro differentiation toward osteo-/odontogenic and angiogenic phenotypes. © 2012 American Association of Endodontists.
Persistent Identifierhttp://hdl.handle.net/10722/146835
ISSN
2015 Impact Factor: 2.904
2015 SCImago Journal Rankings: 1.681
ISI Accession Number ID
Funding AgencyGrant Number
GRF from Research Grants Council of Hong KongHKU 785010M
Funding Information:

Supported by GRF grants from the Research Grants Council of Hong Kong (grant number: HKU 785010M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorDissanayaka, WLen_HK
dc.contributor.authorZhan, Xen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorHargreaves, KMen_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorTong, EHYen_HK
dc.date.accessioned2012-05-23T05:28:08Z-
dc.date.available2012-05-23T05:28:08Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Endodontics, 2012, v. 38 n. 4, p. 454-463en_HK
dc.identifier.issn0099-2399en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146835-
dc.description.abstractIntroduction: Dental pulp stem cells (DPSCs) have received much attention as a promising population of stem cells in regenerative endodontics. Securing a good blood supply during regeneration is a challenging task because of the constricted apical canal opening, which allows only a limited blood supply. The aim of this study was to investigate any potential synergistic effects of dental pulp stem cells and endothelial cells (ECs) on osteo-/odontogenic and angiogenic differentiation in vitro. Methods: Different ratios of DPSCs and ECs were cultured in direct contact using optimized medium for coculture. The 70% confluent cocultures were incubated in the osteo-/odontogenic differentiation medium for up to 3 weeks. Alkaline phosphatase (ALP) activity, the expression levels of ALP, bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP) genes, and alizarin red staining for mineralization at different time points were analyzed. The tubular network formation on Matrigel and the gene expression levels of CD117, VEGF, CD34, and Flk-1 were used as assays to analyze angiogenesis. Results: The quantification of ALP in DPSC:EC cocultures revealed a greater ALP activity compared with DPSC-alone cultures. At all the time points, 1:1 cultures showed a significantly greater ALP activity than that of DPSC-alone cultures. Alizarin red staining and quantification revealed a much greater amount of calcification in the 1:1 and 1:5 cocultures compared with other cultures (P <.01). The expression levels of ALP, BSP, and DSPP genes further confirmed the greater osteo-/odontogenic differentiation in cocultures compared with those of DPSC-alone cultures. Matrigel assay showed that the addition of DPSCs stabilized preexisting vessel-like structures formed by ECs and increased the longevity of them. Conclusions: Direct coculture of DPSCs and ECs enhances the in vitro differentiation toward osteo-/odontogenic and angiogenic phenotypes. © 2012 American Association of Endodontists.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.jendodon.comen_HK
dc.relation.ispartofJournal of Endodonticsen_HK
dc.subjectDental pulp stem cellsen_HK
dc.subjectendothelial cellsen_HK
dc.subjectregenerative endodonticsen_HK
dc.subjectsynergismen_HK
dc.titleCoculture of dental pulp stem cells with endothelial cells enhances osteo-/odontogenic and angiogenic potential in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.joen.2011.12.024en_HK
dc.identifier.pmid22414829-
dc.identifier.scopuseid_2-s2.0-84862797487-
dc.identifier.hkuros199779en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858337948&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue4en_HK
dc.identifier.spage454en_HK
dc.identifier.epage463en_HK
dc.identifier.eissn1878-3554-
dc.identifier.isiWOS:000302926200008-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectSynergistic effects of endothelial progenitor cells and apical papilla stem cells on dental pulp regeneration-
dc.identifier.scopusauthoridDissanayaka, WL=36196419000en_HK
dc.identifier.scopusauthoridZhan, X=54908728000en_HK
dc.identifier.scopusauthoridZhang, C=54908586900en_HK
dc.identifier.scopusauthoridHargreaves, KM=7006655213en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridTong, EHY=54908752000en_HK

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