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Article: Alveolar type II cell-fibroblast interactions, synthesis and secretion of surfactant and type I collagen

TitleAlveolar type II cell-fibroblast interactions, synthesis and secretion of surfactant and type I collagen
Authors
KeywordsAlveolar type II cell
Collagen type I
Fibroblasts
Insulin-like growth factor
Surfactant
Issue Date1993
PublisherThe Company of Biologists Ltd.
Citation
Journal Of Cell Science, 1993, v. 105 n. 2, p. 423-432 How to Cite?
AbstractDuring alveolar development and alveolar repair close contacts are established between fibroblasts and lung epithelial cells through gaps in the basement membrane. Using co-culture systems we have investigated whether these close contacts influence synthesis and secretion of the principal surfactant apoprotein (SP-A) by cultured rat lung alveolar type II cells and the synthesis and secretion of type I collagen by fibroblasts. The alveolar type II cells remained cuboidal and grew in colonies on fibroblast feeder layers and on Matrigel-coated cell culture inserts but were progressively more flattened on fixed fibroblast monolayers and plastic. Alveolar type II cells cultured on plastic released almost all their SP-A into the medium by 4 days. Alveolar type II cells cultured on viable fibroblasts or Matrigel-coated inserts above fibroblasts accumulated SP-A in the medium at a constant rate for the first 4 days, and probably recycle SP-A by endocytosis. The amount of mRNA for SP-A was very low after 4 days of culture of alveolar type II cells on plastic, Matrigel-coated inserts or fixed fibroblast monolayers: relatively, the amount of mRNA for SP-A was increased 4-fold after culture of alveolar type II cells on viable fibroblasts. Co-culture of alveolar type II cells with confluent human dermal fibroblasts stimulated by 2- to 3-fold the secretion of collagen type I into the culture medium, even after the fibroblasts' growth had been arrested with mitomycin C. Collagen secretion, by fibroblasts, also was stimulated 2-fold by conditioned medium from alveolar type II cells cultured on Matrigel. The amount of mRNA for type I collagen increased only modestly when fibroblasts were cultured in this conditioned medium. This stimulation of type I collagen secretion diminished as the conditioned medium was diluted out, but at high dilutions further stimulation occurred, indicating that a factor that inhibited collagen secretion also was being diluted out. The conditioned medium contained low levels of IGF-1 and the stimulation of type I collagen secretion was abolished when the conditioned medium was pre-incubated with antibodies to insulin-like growth factor 1 (IGF-1). There are important reciprocal interactions between alveolar type II cells and fibroblasts in co-culture. Direct contacts between alveolar type II cells and fibroblasts appear to have a trophic effect on cultured alveolar type II cells, increasing the levels of mRNA for SP-A. Rat lung alveolar type II cells appear to release a factor (possibly IGF-1) that stimulates type I collagen secretion by fibroblasts.
Persistent Identifierhttp://hdl.handle.net/10722/146816
ISSN
2015 Impact Factor: 4.706
2015 SCImago Journal Rankings: 3.501
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGriffin, Men_HK
dc.contributor.authorBhandari, Ren_HK
dc.contributor.authorHamilton, Gen_HK
dc.contributor.authorChan, YCen_HK
dc.contributor.authorPowell, JTen_HK
dc.date.accessioned2012-05-22T07:59:29Z-
dc.date.available2012-05-22T07:59:29Z-
dc.date.issued1993en_HK
dc.identifier.citationJournal Of Cell Science, 1993, v. 105 n. 2, p. 423-432en_HK
dc.identifier.issn0021-9533en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146816-
dc.description.abstractDuring alveolar development and alveolar repair close contacts are established between fibroblasts and lung epithelial cells through gaps in the basement membrane. Using co-culture systems we have investigated whether these close contacts influence synthesis and secretion of the principal surfactant apoprotein (SP-A) by cultured rat lung alveolar type II cells and the synthesis and secretion of type I collagen by fibroblasts. The alveolar type II cells remained cuboidal and grew in colonies on fibroblast feeder layers and on Matrigel-coated cell culture inserts but were progressively more flattened on fixed fibroblast monolayers and plastic. Alveolar type II cells cultured on plastic released almost all their SP-A into the medium by 4 days. Alveolar type II cells cultured on viable fibroblasts or Matrigel-coated inserts above fibroblasts accumulated SP-A in the medium at a constant rate for the first 4 days, and probably recycle SP-A by endocytosis. The amount of mRNA for SP-A was very low after 4 days of culture of alveolar type II cells on plastic, Matrigel-coated inserts or fixed fibroblast monolayers: relatively, the amount of mRNA for SP-A was increased 4-fold after culture of alveolar type II cells on viable fibroblasts. Co-culture of alveolar type II cells with confluent human dermal fibroblasts stimulated by 2- to 3-fold the secretion of collagen type I into the culture medium, even after the fibroblasts' growth had been arrested with mitomycin C. Collagen secretion, by fibroblasts, also was stimulated 2-fold by conditioned medium from alveolar type II cells cultured on Matrigel. The amount of mRNA for type I collagen increased only modestly when fibroblasts were cultured in this conditioned medium. This stimulation of type I collagen secretion diminished as the conditioned medium was diluted out, but at high dilutions further stimulation occurred, indicating that a factor that inhibited collagen secretion also was being diluted out. The conditioned medium contained low levels of IGF-1 and the stimulation of type I collagen secretion was abolished when the conditioned medium was pre-incubated with antibodies to insulin-like growth factor 1 (IGF-1). There are important reciprocal interactions between alveolar type II cells and fibroblasts in co-culture. Direct contacts between alveolar type II cells and fibroblasts appear to have a trophic effect on cultured alveolar type II cells, increasing the levels of mRNA for SP-A. Rat lung alveolar type II cells appear to release a factor (possibly IGF-1) that stimulates type I collagen secretion by fibroblasts.en_HK
dc.languageeng-
dc.publisherThe Company of Biologists Ltd.-
dc.relation.ispartofJournal of Cell Scienceen_HK
dc.subjectAlveolar type II cellen_HK
dc.subjectCollagen type Ien_HK
dc.subjectFibroblastsen_HK
dc.subjectInsulin-like growth factoren_HK
dc.subjectSurfactanten_HK
dc.subject.meshApoproteins - biosynthesis - secretion-
dc.subject.meshCollagen - biosynthesis - secretion-
dc.subject.meshFibroblasts - drug effects - metabolism - secretion-
dc.subject.meshIntercellular Junctions - physiology-
dc.subject.meshPulmonary Alveoli - cytology - metabolism - secretion-
dc.titleAlveolar type II cell-fibroblast interactions, synthesis and secretion of surfactant and type I collagenen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, YC: ycchan88@hkucc.hku.hken_HK
dc.identifier.authorityChan, YC=rp00530en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid8408275-
dc.identifier.scopuseid_2-s2.0-0027320001en_HK
dc.identifier.volume105en_HK
dc.identifier.issue2en_HK
dc.identifier.spage423en_HK
dc.identifier.epage432en_HK
dc.identifier.isiWOS:A1993LN98100015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGriffin, M=7403310435en_HK
dc.identifier.scopusauthoridBhandari, R=7005192993en_HK
dc.identifier.scopusauthoridHamilton, G=19337209800en_HK
dc.identifier.scopusauthoridChan, YC=27170769400en_HK
dc.identifier.scopusauthoridPowell, JT=35371486600en_HK

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