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Article: Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods

TitleDetection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
Authors
KeywordsImmunohistochemistry
Influenza
Nucleoprotein
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal of Virological Methods, 2012, v. 179 n. 2, p. 409-413 How to Cite?
Abstract
Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/146790
ISSN
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
ISI Accession Number ID
Funding AgencyGrant Number
University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
European CommissionFP7-GA258084
Funding Information:

We thank Dr. Richard Webby for providing us with the A/Swine/Arkansas/2976/02 (H1N2) virus. We acknowledge AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government. Additional funding was from a grant of the European Commission (FP7-GA258084).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorWong, LPWen_HK
dc.contributor.authorChan, RWYen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorSo, LKYen_HK
dc.contributor.authorYen, HLen_HK
dc.contributor.authorFung, Ken_HK
dc.contributor.authorVan Poucke, Sen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2012-05-10T09:16:10Z-
dc.date.available2012-05-10T09:16:10Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal of Virological Methods, 2012, v. 179 n. 2, p. 409-413en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146790-
dc.description.abstractTissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectInfluenzaen_HK
dc.subjectNucleoproteinen_HK
dc.titleDetection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methodsen_HK
dc.typeArticleen_HK
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailChan, RWY: reneewy@hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailYen, HL: hyen@hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityChan, RWY=rp01596en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityYen, HL=rp00304en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jviromet.2011.11.006en_HK
dc.identifier.pmid22108429en_HK
dc.identifier.scopuseid_2-s2.0-84855518908en_HK
dc.identifier.hkuros202469-
dc.identifier.hkuros205750-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855518908&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume179en_HK
dc.identifier.issue2en_HK
dc.identifier.spage409en_HK
dc.identifier.epage413en_HK
dc.identifier.isiWOS:000300536300018-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridWong, LPW=54418218300en_HK
dc.identifier.scopusauthoridChan, RWY=26661379100en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridSo, LKY=54418003000en_HK
dc.identifier.scopusauthoridYen, HL=7102476668en_HK
dc.identifier.scopusauthoridFung, K=16180183300en_HK
dc.identifier.scopusauthoridVan Poucke, S=35306149100en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.citeulike10058734-

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