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Article: Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
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TitleDetection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
 
AuthorsNicholls, JM1
Wong, LPW1
Chan, RWY1
Poon, LLM1
So, LKY1
Yen, HL1 3
Fung, K1
Van Poucke, S2
Peiris, JSM1 3
 
KeywordsImmunohistochemistry
Influenza
Nucleoprotein
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
CitationJournal of Virological Methods, 2012, v. 179 n. 2, p. 409-413 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2011.11.006
 
AbstractTissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde. © 2011 Elsevier B.V.
 
ISSN0166-0934
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
 
DOIhttp://dx.doi.org/10.1016/j.jviromet.2011.11.006
 
ISI Accession Number IDWOS:000300536300018
Funding AgencyGrant Number
University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
European CommissionFP7-GA258084
Funding Information:

We thank Dr. Richard Webby for providing us with the A/Swine/Arkansas/2976/02 (H1N2) virus. We acknowledge AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government. Additional funding was from a grant of the European Commission (FP7-GA258084).

 
ReferencesReferences in Scopus
 
GrantsControl of Pandemic and Inter-pandemic Influenza
 
DC FieldValue
dc.contributor.authorNicholls, JM
 
dc.contributor.authorWong, LPW
 
dc.contributor.authorChan, RWY
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorSo, LKY
 
dc.contributor.authorYen, HL
 
dc.contributor.authorFung, K
 
dc.contributor.authorVan Poucke, S
 
dc.contributor.authorPeiris, JSM
 
dc.date.accessioned2012-05-10T09:16:10Z
 
dc.date.available2012-05-10T09:16:10Z
 
dc.date.issued2012
 
dc.description.abstractTissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde. © 2011 Elsevier B.V.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal of Virological Methods, 2012, v. 179 n. 2, p. 409-413 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jviromet.2011.11.006
 
dc.identifier.citeulike10058734
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jviromet.2011.11.006
 
dc.identifier.epage413
 
dc.identifier.hkuros202469
 
dc.identifier.hkuros205750
 
dc.identifier.isiWOS:000300536300018
Funding AgencyGrant Number
University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
European CommissionFP7-GA258084
Funding Information:

We thank Dr. Richard Webby for providing us with the A/Swine/Arkansas/2976/02 (H1N2) virus. We acknowledge AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government. Additional funding was from a grant of the European Commission (FP7-GA258084).

 
dc.identifier.issn0166-0934
2013 Impact Factor: 1.883
2013 SCImago Journal Rankings: 0.860
 
dc.identifier.issue2
 
dc.identifier.pmid22108429
 
dc.identifier.scopuseid_2-s2.0-84855518908
 
dc.identifier.spage409
 
dc.identifier.urihttp://hdl.handle.net/10722/146790
 
dc.identifier.volume179
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Virological Methods
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.referencesReferences in Scopus
 
dc.subjectImmunohistochemistry
 
dc.subjectInfluenza
 
dc.subjectNucleoprotein
 
dc.titleDetection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Universiteit Gent
  3. HKU-Pasteur Research Centre