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Article: Tissue tropism of swine influenza viruses and reassortants in ex vivo cultures of the human respiratory tract and conjunctiva
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TitleTissue tropism of swine influenza viruses and reassortants in ex vivo cultures of the human respiratory tract and conjunctiva
 
AuthorsChan, RWY1
Kang, SSR1
Yen, HL1 2
Li, ACL1
Tang, LLS1
Yu, WCL1
Yuen, KM1
Chan, IWW1
Wong, DDY1 2
Lai, WW1
Kwong, DLW1
Sihoe, ADL1
Poon, LLM1
Guan, Y1
Nicholls, JM1
Peiris, JSM1 2
Chan, MCW1
 
Issue Date2011
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
CitationJournal of Virology, 2011, v. 85 n. 22, p. 11581-11587 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.05662-11
 
AbstractThe 2009 pandemic influenza H1N1 (H1N1pdm) virus was generated by reassortment of swine influenza viruses of different lineages. This was the first influenza pandemic to emerge in over 4 decades and the first to occur after the realization that influenza pandemics arise from influenza viruses of animals. In order to understand the biological determinants of pandemic emergence, it is relevant to compare the tropism of different lineages of swine influenza viruses and reassortants derived from them with that of 2009 pandemic H1N1 (H1N1pdm) and seasonal influenza H1N1 viruses in ex vivo cultures of the human nasopharynx, bronchus, alveoli, and conjunctiva. We hypothesized that virus which can transmit efficiently between humans replicated well in the human upper airways. As previously reported, H1N1pdm and seasonal H1N1 viruses replicated efficiently in the nasopharyngeal, bronchial, and alveolar epithelium. In contrast, representative viruses from the classical swine (CS) (H1N1) lineage could not infect human respiratory epithelium; Eurasian avian-like swine (EA) (H1N1) viruses only infected alveolar epithelium and North American triple-reassortant (TRIG) viruses only infected the bronchial epithelium albeit inefficiently. Interestingly, a naturally occurring triple-reassortant swine virus, A/SW/HK/915/04 (H1N2), with a matrix gene segment of EA swine derivation (i.e., differing from H1N1pdm only in lacking a neuraminidase [NA] gene of EA derivation) readily infected and replicated in human nasopharyngeal and bronchial epithelia but not in the lung. A recombinant sw915 with the NA from H1N1pdm retained its tropism for the bronchus and acquired additional replication competence for alveolar epithelium. In contrast to H1N1pdm, none of the swine viruses tested nor seasonal H1N1 had tropism in human conjunctiva. Recombinant viruses generated by swapping the surface proteins (hemagglutinin and NA) of H1N1pdm and seasonal H1N1 virus demonstrated that these two gene segments together are key determinants of conjunctival tropism. Overall, these findings suggest that ex vivo cultures of the human respiratory tract provide a useful biological model for assessing the human health risk of swine influenza viruses. © 2011, American Society for Microbiology.
 
ISSN0022-538X
2013 Impact Factor: 4.648
 
DOIhttp://dx.doi.org/10.1128/JVI.05662-11
 
PubMed Central IDPMC3209323
 
ISI Accession Number IDWOS:000296422700004
Funding AgencyGrant Number
commissioned study
RFCID10090202
10091132
Infectious Disease, Food and Health Bureau, Hong Kong SAR Government
General Research FundHKU 7612/08
Research Grants Council, Hong Kong SAR Government
National Institutes of Health (NIAID )HHSN266200700005C
University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
European CommissionFP7-GA258084
Funding Information:

We acknowledge financial support by a commissioned study (to J.S.M.P.) and RFCID grants (reference numbers 10090202 and 10091132 to J.M.N. and R.W.Y.C., respectively) by the Research Fund for Control of Infectious Disease, Food and Health Bureau, Hong Kong SAR Government, and the General Research Fund (HKU 7612/08 M to M.C.W. C.), Research Grants Council, Hong Kong SAR Government; the National Institutes of Health (NIAID contract HHSN266200700005C); and AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government. Additional funding was provided by a grant from the European Commission (FP7-GA258084).

 
ReferencesReferences in Scopus
 
GrantsPrevention and treatment of swine origin influenza virus (S-OIV) though the use of interferon - an in vivo and ex vivo study
The biological and genetic determinants of seasonal influenza A virus replication in the lower respiratory tract
Control of Pandemic and Inter-pandemic Influenza
 
DC FieldValue
dc.contributor.authorChan, RWY
 
dc.contributor.authorKang, SSR
 
dc.contributor.authorYen, HL
 
dc.contributor.authorLi, ACL
 
dc.contributor.authorTang, LLS
 
dc.contributor.authorYu, WCL
 
dc.contributor.authorYuen, KM
 
dc.contributor.authorChan, IWW
 
dc.contributor.authorWong, DDY
 
dc.contributor.authorLai, WW
 
dc.contributor.authorKwong, DLW
 
dc.contributor.authorSihoe, ADL
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorNicholls, JM
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorChan, MCW
 
dc.date.accessioned2012-05-10T09:16:10Z
 
dc.date.available2012-05-10T09:16:10Z
 
dc.date.issued2011
 
dc.description.abstractThe 2009 pandemic influenza H1N1 (H1N1pdm) virus was generated by reassortment of swine influenza viruses of different lineages. This was the first influenza pandemic to emerge in over 4 decades and the first to occur after the realization that influenza pandemics arise from influenza viruses of animals. In order to understand the biological determinants of pandemic emergence, it is relevant to compare the tropism of different lineages of swine influenza viruses and reassortants derived from them with that of 2009 pandemic H1N1 (H1N1pdm) and seasonal influenza H1N1 viruses in ex vivo cultures of the human nasopharynx, bronchus, alveoli, and conjunctiva. We hypothesized that virus which can transmit efficiently between humans replicated well in the human upper airways. As previously reported, H1N1pdm and seasonal H1N1 viruses replicated efficiently in the nasopharyngeal, bronchial, and alveolar epithelium. In contrast, representative viruses from the classical swine (CS) (H1N1) lineage could not infect human respiratory epithelium; Eurasian avian-like swine (EA) (H1N1) viruses only infected alveolar epithelium and North American triple-reassortant (TRIG) viruses only infected the bronchial epithelium albeit inefficiently. Interestingly, a naturally occurring triple-reassortant swine virus, A/SW/HK/915/04 (H1N2), with a matrix gene segment of EA swine derivation (i.e., differing from H1N1pdm only in lacking a neuraminidase [NA] gene of EA derivation) readily infected and replicated in human nasopharyngeal and bronchial epithelia but not in the lung. A recombinant sw915 with the NA from H1N1pdm retained its tropism for the bronchus and acquired additional replication competence for alveolar epithelium. In contrast to H1N1pdm, none of the swine viruses tested nor seasonal H1N1 had tropism in human conjunctiva. Recombinant viruses generated by swapping the surface proteins (hemagglutinin and NA) of H1N1pdm and seasonal H1N1 virus demonstrated that these two gene segments together are key determinants of conjunctival tropism. Overall, these findings suggest that ex vivo cultures of the human respiratory tract provide a useful biological model for assessing the human health risk of swine influenza viruses. © 2011, American Society for Microbiology.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal of Virology, 2011, v. 85 n. 22, p. 11581-11587 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.05662-11
 
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.05662-11
 
dc.identifier.eissn1098-5514
 
dc.identifier.epage11587
 
dc.identifier.hkuros202478
 
dc.identifier.isiWOS:000296422700004
Funding AgencyGrant Number
commissioned study
RFCID10090202
10091132
Infectious Disease, Food and Health Bureau, Hong Kong SAR Government
General Research FundHKU 7612/08
Research Grants Council, Hong Kong SAR Government
National Institutes of Health (NIAID )HHSN266200700005C
University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
European CommissionFP7-GA258084
Funding Information:

We acknowledge financial support by a commissioned study (to J.S.M.P.) and RFCID grants (reference numbers 10090202 and 10091132 to J.M.N. and R.W.Y.C., respectively) by the Research Fund for Control of Infectious Disease, Food and Health Bureau, Hong Kong SAR Government, and the General Research Fund (HKU 7612/08 M to M.C.W. C.), Research Grants Council, Hong Kong SAR Government; the National Institutes of Health (NIAID contract HHSN266200700005C); and AoE Funding (AoE/M-12/06) from the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government. Additional funding was provided by a grant from the European Commission (FP7-GA258084).

 
dc.identifier.issn0022-538X
2013 Impact Factor: 4.648
 
dc.identifier.issue22
 
dc.identifier.pmcidPMC3209323
 
dc.identifier.pmid21880750
 
dc.identifier.scopuseid_2-s2.0-80655143499
 
dc.identifier.spage11581
 
dc.identifier.urihttp://hdl.handle.net/10722/146789
 
dc.identifier.volume85
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Virology
 
dc.relation.projectPrevention and treatment of swine origin influenza virus (S-OIV) though the use of interferon - an in vivo and ex vivo study
 
dc.relation.projectThe biological and genetic determinants of seasonal influenza A virus replication in the lower respiratory tract
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshConjunctiva - Virology
 
dc.subject.meshHumans
 
dc.subject.meshInfluenza A Virus, H1n1 Subtype - Genetics - Growth & Development - Pathogenicity
 
dc.subject.meshInfluenza A Virus, H1n2 Subtype - Genetics - Growth & Development - Pathogenicity
 
dc.subject.meshOrgan Culture Techniques
 
dc.subject.meshReassortant Viruses - Isolation & Purification
 
dc.subject.meshRespiratory Mucosa - Virology
 
dc.subject.meshSwine
 
dc.subject.meshViral Tropism
 
dc.subject.meshVirulence
 
dc.titleTissue tropism of swine influenza viruses and reassortants in ex vivo cultures of the human respiratory tract and conjunctiva
 
dc.typeArticle
 
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Author Affiliations
  1. Queen Mary Hospital Hong Kong
  2. HKU-Pasteur Research Centre