File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Dual functions of ginsenosides in protecting human endothelial cells against influenza H9N2-induced inflammation and apoptosis

TitleDual functions of ginsenosides in protecting human endothelial cells against influenza H9N2-induced inflammation and apoptosis
Authors
KeywordsEndothelial cell
Ginsenoside
Influenza
IP-11
miR-15b
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2011, v. 137 n. 3, p. 1542-1546 How to Cite?
AbstractEthnopharmacological relevance: Panax ginseng is a precious traditional Chinese herbal medicine which has been utilized as herbal tonic for improving immunity. The active component, ginsenosides have been shown to possess various pharmacological functions including immunomodulation and cardiovascular protection. Aim of the study: To investigate the immunomodulatory effect and anti-apoptotic effect of ginsenosides on avian influenza-infected human endothelial cells, and to present evidence for the cardiovascular protection by ginseng during influenza infection. Materials and methods: Human umbilical vein endothelial cells (HUVECs) were infected with avian influenza H9N2/G1 to induce IP-10 production and cell death, cells were then incubated with ginsenosides PPT and Re. The level of IP-10 and microRNA was determined by ELISA and real-time PCR respectively. Cell death was determined by MTT, TUNEL and flow cytometry. Results: Ginsenoside metabolite protopanaxatriol showed significant suppression effect on IP-10 production upon H9N2/G1 infection through up-regulation of miR-15b expression. In addition, ginsenoside-induced cytoprotection was reflected in the increase of cell viability. Data from flow cytometry analysis and TUNEL assay also showed that ginsenoside Re could protect ECs from H9N2/G1-induced apoptosis and DNA damage. Conclusions: This report further supports the traditional belief for immunomodulatory effects of ginseng, also demonstrated the partial protective mechanism of ginsenosides on avian influenza infection and its related endothelial dysfunction. © 2011 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/146788
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
Funding AgencyGrant Number
Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR GovernmentAoE/M-12/06
Research Grant CommitteeHKBU 261810
Hong Kong SAR Government
Hong Kong Baptist UniversityFRG1/10-11/027
FRG2/09-10/068
Funding Information:

This work was supported by the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR Government (AoE/M-12/06), General Research Fund (HKBU 261810) of the Research Grant Committee, Hong Kong SAR Government and Faculty Research Grant (FRG1/10-11/027 and FRG2/09-10/068) of the Hong Kong Baptist University.(FRG1/10-11/027 and FRG2/09-10/068)

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorChan, LYen_HK
dc.contributor.authorKwok, HHen_HK
dc.contributor.authorChan, RWYen_HK
dc.contributor.authorPeiris, MJSen_HK
dc.contributor.authorMak, NKen_HK
dc.contributor.authorWong, RNSen_HK
dc.contributor.authorChan, MCWen_HK
dc.contributor.authorYue, PYKen_HK
dc.date.accessioned2012-05-10T09:16:09Z-
dc.date.available2012-05-10T09:16:09Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2011, v. 137 n. 3, p. 1542-1546en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146788-
dc.description.abstractEthnopharmacological relevance: Panax ginseng is a precious traditional Chinese herbal medicine which has been utilized as herbal tonic for improving immunity. The active component, ginsenosides have been shown to possess various pharmacological functions including immunomodulation and cardiovascular protection. Aim of the study: To investigate the immunomodulatory effect and anti-apoptotic effect of ginsenosides on avian influenza-infected human endothelial cells, and to present evidence for the cardiovascular protection by ginseng during influenza infection. Materials and methods: Human umbilical vein endothelial cells (HUVECs) were infected with avian influenza H9N2/G1 to induce IP-10 production and cell death, cells were then incubated with ginsenosides PPT and Re. The level of IP-10 and microRNA was determined by ELISA and real-time PCR respectively. Cell death was determined by MTT, TUNEL and flow cytometry. Results: Ginsenoside metabolite protopanaxatriol showed significant suppression effect on IP-10 production upon H9N2/G1 infection through up-regulation of miR-15b expression. In addition, ginsenoside-induced cytoprotection was reflected in the increase of cell viability. Data from flow cytometry analysis and TUNEL assay also showed that ginsenoside Re could protect ECs from H9N2/G1-induced apoptosis and DNA damage. Conclusions: This report further supports the traditional belief for immunomodulatory effects of ginseng, also demonstrated the partial protective mechanism of ginsenosides on avian influenza infection and its related endothelial dysfunction. © 2011 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectEndothelial cellen_HK
dc.subjectGinsenosideen_HK
dc.subjectInfluenzaen_HK
dc.subjectIP-11en_HK
dc.subjectmiR-15ben_HK
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChemokine Cxcl10 - Metabolismen_US
dc.subject.meshCytoprotectionen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGinsenosides - Pharmacologyen_US
dc.subject.meshHuman Umbilical Vein Endothelial Cells - Drug Effects - Immunology - Pathology - Virologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunologic Factors - Pharmacologyen_US
dc.subject.meshIn Situ Nick-End Labelingen_US
dc.subject.meshInflammation Mediators - Metabolismen_US
dc.subject.meshInfluenza A Virus, H9n2 Subtype - Pathogenicityen_US
dc.subject.meshMicrornas - Metabolismen_US
dc.subject.meshReal-Time Polymerase Chain Reactionen_US
dc.subject.meshSapogenins - Pharmacologyen_US
dc.subject.meshTransfectionen_US
dc.titleDual functions of ginsenosides in protecting human endothelial cells against influenza H9N2-induced inflammation and apoptosisen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, RWY: reneewy@hku.hken_HK
dc.identifier.emailPeiris, MJS: malik@hkucc.hku.hken_HK
dc.identifier.emailChan, MCW: mchan@hku.hken_HK
dc.identifier.authorityChan, RWY=rp01596en_HK
dc.identifier.authorityPeiris, MJS=rp00410en_HK
dc.identifier.authorityChan, MCW=rp00420en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jep.2011.08.022en_HK
dc.identifier.pmid21872652-
dc.identifier.scopuseid_2-s2.0-80054919768en_HK
dc.identifier.hkuros202479-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054919768&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume137en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1542en_HK
dc.identifier.epage1546en_HK
dc.identifier.isiWOS:000297387000071-
dc.publisher.placeIrelanden_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridChan, LY=54395088200en_HK
dc.identifier.scopusauthoridKwok, HH=36523022200en_HK
dc.identifier.scopusauthoridChan, RWY=26661379100en_HK
dc.identifier.scopusauthoridPeiris, MJS=7005486823en_HK
dc.identifier.scopusauthoridMak, NK=35587830100en_HK
dc.identifier.scopusauthoridWong, RNS=7402126957en_HK
dc.identifier.scopusauthoridChan, MCW=26654715500en_HK
dc.identifier.scopusauthoridYue, PYK=8570616200en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats