File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Overexpression of Na+-dependent myo-inositol transporter gene mouse lens led to congenital cataract

TitleOverexpression of Na+-dependent myo-inositol transporter gene mouse lens led to congenital cataract
Authors
Issue Date2000
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2000, v. 41 n. 6, p. 1467-1472 How to Cite?
AbstractPURPOSE. Maintaining appropriate osmotic pressure is essential for maintaining lens transparency. This study was performed to investigate whether high levels of myo-inositol, one of the major organic osmolytes in the lens, would lead to cataract development. METHODS. Transgenic mouse lines carrying the bovine Na+-dependent myo-inositol transporter (bSMIT) cDNA under the control of the mouse αA-crystallin promoter were generated. RESULTS. Increased bSMIT expression was accompanied by increased myo-inositol level in the lens and increased uptake of (3H) myo-inositol by the lens in culture. The transgenic mice developed observable cataract under normal rearing conditions beginning at 2 to 8 weeks of age, and the severity of cataract development was correlated to the level of bSMIT gene expression and lens myo-inositol accumulation. For transgenic mouse line 3352, heterozygous mice did not develop cataract, whereas homozygous ones did. Prenatal feeding of heterozygous 3352 mice with high myo-inositol diet led to cataract development, indicating that cataract development was not merely due to a nonspecific effect of SMIT overexpression. Introducing aldose reductase overexpressing transgene into heterozygous 3352 mice also led to cataract development, indicating that this type of cataract is primarily due to osmotic stress. CONCLUSIONS. The present results indicate that high levels of myo-inositol and sorbitol in the lens contribute to cataract development. This is a useful model to study the role of osmotic stress in cataractogenesis during lens development.
Persistent Identifierhttp://hdl.handle.net/10722/146627
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Zen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorZhou, Cen_HK
dc.contributor.authorCammarata, PRen_HK
dc.contributor.authorChung, SSMen_HK
dc.date.accessioned2012-05-08T03:21:21Z-
dc.date.available2012-05-08T03:21:21Z-
dc.date.issued2000en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2000, v. 41 n. 6, p. 1467-1472en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146627-
dc.description.abstractPURPOSE. Maintaining appropriate osmotic pressure is essential for maintaining lens transparency. This study was performed to investigate whether high levels of myo-inositol, one of the major organic osmolytes in the lens, would lead to cataract development. METHODS. Transgenic mouse lines carrying the bovine Na+-dependent myo-inositol transporter (bSMIT) cDNA under the control of the mouse αA-crystallin promoter were generated. RESULTS. Increased bSMIT expression was accompanied by increased myo-inositol level in the lens and increased uptake of (3H) myo-inositol by the lens in culture. The transgenic mice developed observable cataract under normal rearing conditions beginning at 2 to 8 weeks of age, and the severity of cataract development was correlated to the level of bSMIT gene expression and lens myo-inositol accumulation. For transgenic mouse line 3352, heterozygous mice did not develop cataract, whereas homozygous ones did. Prenatal feeding of heterozygous 3352 mice with high myo-inositol diet led to cataract development, indicating that cataract development was not merely due to a nonspecific effect of SMIT overexpression. Introducing aldose reductase overexpressing transgene into heterozygous 3352 mice also led to cataract development, indicating that this type of cataract is primarily due to osmotic stress. CONCLUSIONS. The present results indicate that high levels of myo-inositol and sorbitol in the lens contribute to cataract development. This is a useful model to study the role of osmotic stress in cataractogenesis during lens development.en_HK
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAldehyde Reductase - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCarrier Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshCataract - Congenital - Metabolism - Pathologyen_US
dc.subject.meshDietary Carbohydrates - Administration & Dosageen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHeat-Shock Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshInositol - Biosynthesisen_US
dc.subject.meshLens, Crystalline - Metabolism - Pathologyen_US
dc.subject.meshMembrane Proteinsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Inbred Cbaen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshOsmotic Pressureen_US
dc.subject.meshSorbitol - Metabolismen_US
dc.subject.meshSymportersen_US
dc.titleOverexpression of Na+-dependent myo-inositol transporter gene mouse lens led to congenital cataracten_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid10798664-
dc.identifier.scopuseid_2-s2.0-0042748904en_HK
dc.identifier.hkuros53075-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042748904&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume41en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1467en_HK
dc.identifier.epage1472en_HK
dc.identifier.isiWOS:000086800800027-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJiang, Z=7404279744en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridZhou, C=36726307900en_HK
dc.identifier.scopusauthoridCammarata, PR=7003885598en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats