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Article: Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development

TitleMouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development
Authors
KeywordscDNA cloning
Development
Hybridization (in situ)
Mouse
Preproendothelin-1
Issue Date1995
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1995, v. 234 n. 3, p. 819-826 How to Cite?
AbstractEndothelin-1 (ET-1) is a peptide implicated in a wide variety of functions involving vascular and non-vascular systems. We have cloned the cDNA encoding the mouse prepro-endothelin-1 (PPET-1) and determined its nucleotide sequence. The putative PPET-1 peptide processing sites are all conserved and the deduced 21-amino-acid mature ET-1 peptide is identical to that of the rat, human, bovine, porcine and rabbit. Using the cloned cDNA as a probe for in situ hybridization, we detected PPET-1 mRNA in different tissues at different stages of mouse embryonic development. Embryos at a stage as early as 9.5 days postcoitum (E9.5) have very strong expression in the branchial epithelium, optic vesicle and the endothelial cells of large blood vessels, including the dorsal aorta and aortic arches. While the expression level in the branchial epithelium was decreasing towards the later stage of embryogenesis, the expression in the endothelial cells increased with age. At E10.5, PPET-1 mRNA was also detected in the otic vesicle as well as in the developing gut epithelium. At later stage of development, the expression of PPET-1 was primarily found in the vascular endothelial cells, cochlea, eye and the gut, with the highest level of PPET-1 mRNA in the endothelial cells of the lung. These data will be useful for analyzing the function of ET-1 in these organs.
Persistent Identifierhttp://hdl.handle.net/10722/146623
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, TSKen_HK
dc.contributor.authorLin, CXFen_HK
dc.contributor.authorChan, WYen_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2012-05-08T03:21:20Z-
dc.date.available2012-05-08T03:21:20Z-
dc.date.issued1995en_HK
dc.identifier.citationEuropean Journal Of Biochemistry, 1995, v. 234 n. 3, p. 819-826en_HK
dc.identifier.issn0014-2956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146623-
dc.description.abstractEndothelin-1 (ET-1) is a peptide implicated in a wide variety of functions involving vascular and non-vascular systems. We have cloned the cDNA encoding the mouse prepro-endothelin-1 (PPET-1) and determined its nucleotide sequence. The putative PPET-1 peptide processing sites are all conserved and the deduced 21-amino-acid mature ET-1 peptide is identical to that of the rat, human, bovine, porcine and rabbit. Using the cloned cDNA as a probe for in situ hybridization, we detected PPET-1 mRNA in different tissues at different stages of mouse embryonic development. Embryos at a stage as early as 9.5 days postcoitum (E9.5) have very strong expression in the branchial epithelium, optic vesicle and the endothelial cells of large blood vessels, including the dorsal aorta and aortic arches. While the expression level in the branchial epithelium was decreasing towards the later stage of embryogenesis, the expression in the endothelial cells increased with age. At E10.5, PPET-1 mRNA was also detected in the otic vesicle as well as in the developing gut epithelium. At later stage of development, the expression of PPET-1 was primarily found in the vascular endothelial cells, cochlea, eye and the gut, with the highest level of PPET-1 mRNA in the endothelial cells of the lung. These data will be useful for analyzing the function of ET-1 in these organs.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_HK
dc.relation.ispartofEuropean Journal of Biochemistryen_HK
dc.subjectcDNA cloningen_HK
dc.subjectDevelopmenten_HK
dc.subjectHybridization (in situ)en_HK
dc.subjectMouseen_HK
dc.subjectPreproendothelin-1en_HK
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain - Embryology - Metabolismen_US
dc.subject.meshCardiovascular System - Embryology - Metabolismen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDna, Complementary - Geneticsen_US
dc.subject.meshDigestive System - Embryology - Metabolismen_US
dc.subject.meshEar - Embryologyen_US
dc.subject.meshEmbryonic And Fetal Developmenten_US
dc.subject.meshEndothelin-1en_US
dc.subject.meshEndothelins - Chemistry - Genetics - Metabolismen_US
dc.subject.meshEye - Embryology - Metabolismen_US
dc.subject.meshGene Expression Regulation, Developmental - Geneticsen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLung - Embryology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProtein Precursors - Chemistry - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysisen_US
dc.subject.meshSpinal Cord - Embryology - Metabolismen_US
dc.titleMouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic developmenten_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8575440-
dc.identifier.scopuseid_2-s2.0-0029557483en_HK
dc.identifier.hkuros13599-
dc.identifier.volume234en_HK
dc.identifier.issue3en_HK
dc.identifier.spage819en_HK
dc.identifier.epage826en_HK
dc.identifier.isiWOS:A1995TP56100016-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, TSK=7402687396en_HK
dc.identifier.scopusauthoridLin, CXF=15035068300en_HK
dc.identifier.scopusauthoridChan, WY=37033528800en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK

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