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Article: Cloning, sequencing, and determination of the sites of expression of mouse sorbitol dehydrogenase cDNA

TitleCloning, sequencing, and determination of the sites of expression of mouse sorbitol dehydrogenase cDNA
Authors
KeywordscDNA cloning
in situ hybridization
mouse
Northern-blot hybridization
Sorbitol dehydrogenase
Issue Date1995
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1995, v. 230 n. 3, p. 1059-1065 How to Cite?
AbstractSorbitol dehydrogenase is one of the enzymes in the polyol pathway, which is thought to be implicated in the pathogenesis of diabetic complications. The cDNA encoding mouse sorbitol dehydrogenase was cloned from a liver library and its sequence was determined. The open reading frame encodes a product of 356 amino acids that shares high similarity with the human and rat liver sorbitol dehydrogenases (83% and 93% identity, respectively). The 3'- untranslated region contains a truncated L1Md repeat element inserted in reverse relative to the sorbitol dehydrogenase cDNA. Northern-blot hybridization showed that the testis has the highest level of expression, followed by kidney, liver, and lung. Low levels of expression were also observed in lens, brain, and skeletal muscle. In situ hybridization revealed that in the kidney, the highest concentration of sorbitol dehydrogenase mRNA is observed in the cortex, but is absent from the inner medulla. The parenchymal cells of the liver showed strong expression while the cells of the hepatic vasculature did not hybridize. The sorbitol dehydrogenase expression in the seminiferous tubules was mostly associated with the mature cells of the developing germ cells, confirming the usefulness of sorbitol dehydrogenase as an enzyme indicator for sexual maturation. The seminal vesicle, where most of the seminal fructose is produced, also showed a high level of expression in the epithelial cells. The mouse sorbitol dehydrogenase cDNA will be useful in the studies of the involvement of the polyol pathway in diabetic complications.
Persistent Identifierhttp://hdl.handle.net/10722/146622
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFuk Ki Leeen_HK
dc.contributor.authorLee, AYWen_HK
dc.contributor.authorLin, CXFen_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorSookja Kim Chungen_HK
dc.date.accessioned2012-05-08T03:21:19Z-
dc.date.available2012-05-08T03:21:19Z-
dc.date.issued1995en_HK
dc.identifier.citationEuropean Journal Of Biochemistry, 1995, v. 230 n. 3, p. 1059-1065en_HK
dc.identifier.issn0014-2956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146622-
dc.description.abstractSorbitol dehydrogenase is one of the enzymes in the polyol pathway, which is thought to be implicated in the pathogenesis of diabetic complications. The cDNA encoding mouse sorbitol dehydrogenase was cloned from a liver library and its sequence was determined. The open reading frame encodes a product of 356 amino acids that shares high similarity with the human and rat liver sorbitol dehydrogenases (83% and 93% identity, respectively). The 3'- untranslated region contains a truncated L1Md repeat element inserted in reverse relative to the sorbitol dehydrogenase cDNA. Northern-blot hybridization showed that the testis has the highest level of expression, followed by kidney, liver, and lung. Low levels of expression were also observed in lens, brain, and skeletal muscle. In situ hybridization revealed that in the kidney, the highest concentration of sorbitol dehydrogenase mRNA is observed in the cortex, but is absent from the inner medulla. The parenchymal cells of the liver showed strong expression while the cells of the hepatic vasculature did not hybridize. The sorbitol dehydrogenase expression in the seminiferous tubules was mostly associated with the mature cells of the developing germ cells, confirming the usefulness of sorbitol dehydrogenase as an enzyme indicator for sexual maturation. The seminal vesicle, where most of the seminal fructose is produced, also showed a high level of expression in the epithelial cells. The mouse sorbitol dehydrogenase cDNA will be useful in the studies of the involvement of the polyol pathway in diabetic complications.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_HK
dc.relation.ispartofEuropean Journal of Biochemistryen_HK
dc.subjectcDNA cloningen_HK
dc.subjectin situ hybridizationen_HK
dc.subjectmouseen_HK
dc.subjectNorthern-blot hybridizationen_HK
dc.subjectSorbitol dehydrogenaseen_HK
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Complementary - Chemistryen_US
dc.subject.meshFructose - Biosynthesisen_US
dc.subject.meshL-Iditol 2-Dehydrogenase - Chemistry - Genetics - Physiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.titleCloning, sequencing, and determination of the sites of expression of mouse sorbitol dehydrogenase cDNAen_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailSookja Kim Chung: skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authoritySookja Kim Chung=rp00381en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1111/j.1432-1033.1995.tb20656.xen_HK
dc.identifier.pmid7601136-
dc.identifier.scopuseid_2-s2.0-0029038499en_HK
dc.identifier.hkuros13597-
dc.identifier.volume230en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1059en_HK
dc.identifier.epage1065en_HK
dc.identifier.isiWOS:A1995RF16200030-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridFuk Ki Lee=16180322900en_HK
dc.identifier.scopusauthoridLee, AYW=55477259800en_HK
dc.identifier.scopusauthoridLin, CXF=15035068300en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK
dc.identifier.scopusauthoridSookja Kim Chung=7404292976en_HK

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