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Article: The effect of progesterone on oxytocin messenger RNA in hypothalamic neurons of estrogen-treated female rats studied with quantitative in situ hybridization histochemistry

TitleThe effect of progesterone on oxytocin messenger RNA in hypothalamic neurons of estrogen-treated female rats studied with quantitative in situ hybridization histochemistry
Authors
Issue Date1991
Citation
Biomedical Research, 1991, v. 12 n. 6, p. 405-415 How to Cite?
AbstractUsing in situ hybridization histochemistry with tritiated synthetic oligodeoxynucleotide probes and a computer-assisted image analysis system, we examined possible progesterone effects on oxytocin messenger RNA (mRNA) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the female estrogen-primed rat. Conditions of combined estrogen and progesterone exposures were used to address questions of relevance to the combined effect of these two hormones on female reproductive behavior. Hybridization signals for oxytocin mRNA were shown by control maneuvers to be quite specific, and were observed in neurons of the dorsolateral part of the SON and in the peripheral part of the PVN. Statistic analysis of the Student t-test and Kolmogorov-Smirnov test showed that progesterone treatment of estrogen-primed ovariectomized female rats did not affect hybridization for oxytocin mRNA in neurons of the SON, whereas prolonged progesterone exposure did significantly increase the number of silver grains in neurons of the PVN. Among the subdivisions of the PVN, the posterior ventromedial part showed a particularly strong increase in oxytocin mRNA levels. These results suggest that progesterone, working in concert with estrogen priming, has an effect on oxytocin mRNA levels in that portion of the PVN, which might be relevant for the control of female reproductive behavior.
Persistent Identifierhttp://hdl.handle.net/10722/146618
ISSN
2015 Impact Factor: 0.953
2015 SCImago Journal Rankings: 0.502

 

DC FieldValueLanguage
dc.contributor.authorKawata, Men_HK
dc.contributor.authorMcCabe, JTen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorDutt, Aen_HK
dc.contributor.authorYuri, Ken_HK
dc.contributor.authorHirakawa, Men_HK
dc.contributor.authorKumamoto, Ken_HK
dc.contributor.authorHirayama, Yen_HK
dc.contributor.authorPfaff, DWen_HK
dc.date.accessioned2012-05-08T03:21:18Z-
dc.date.available2012-05-08T03:21:18Z-
dc.date.issued1991en_HK
dc.identifier.citationBiomedical Research, 1991, v. 12 n. 6, p. 405-415en_HK
dc.identifier.issn0388-6107en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146618-
dc.description.abstractUsing in situ hybridization histochemistry with tritiated synthetic oligodeoxynucleotide probes and a computer-assisted image analysis system, we examined possible progesterone effects on oxytocin messenger RNA (mRNA) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the female estrogen-primed rat. Conditions of combined estrogen and progesterone exposures were used to address questions of relevance to the combined effect of these two hormones on female reproductive behavior. Hybridization signals for oxytocin mRNA were shown by control maneuvers to be quite specific, and were observed in neurons of the dorsolateral part of the SON and in the peripheral part of the PVN. Statistic analysis of the Student t-test and Kolmogorov-Smirnov test showed that progesterone treatment of estrogen-primed ovariectomized female rats did not affect hybridization for oxytocin mRNA in neurons of the SON, whereas prolonged progesterone exposure did significantly increase the number of silver grains in neurons of the PVN. Among the subdivisions of the PVN, the posterior ventromedial part showed a particularly strong increase in oxytocin mRNA levels. These results suggest that progesterone, working in concert with estrogen priming, has an effect on oxytocin mRNA levels in that portion of the PVN, which might be relevant for the control of female reproductive behavior.en_HK
dc.languageengen_US
dc.relation.ispartofBiomedical Researchen_HK
dc.titleThe effect of progesterone on oxytocin messenger RNA in hypothalamic neurons of estrogen-treated female rats studied with quantitative in situ hybridization histochemistryen_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0026323130en_HK
dc.identifier.volume12en_HK
dc.identifier.issue6en_HK
dc.identifier.spage405en_HK
dc.identifier.epage415en_HK
dc.identifier.scopusauthoridKawata, M=7103242782en_HK
dc.identifier.scopusauthoridMcCabe, JT=35516007000en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridDutt, A=35822342800en_HK
dc.identifier.scopusauthoridYuri, K=7005715881en_HK
dc.identifier.scopusauthoridHirakawa, M=8744752900en_HK
dc.identifier.scopusauthoridKumamoto, K=7006750188en_HK
dc.identifier.scopusauthoridHirayama, Y=16150295500en_HK
dc.identifier.scopusauthoridPfaff, DW=36046651600en_HK

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