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Article: Estrogen influences on oxytocin mRNA expression in preoptic and anterior hypothalamic regions studied by in situ hybridization

TitleEstrogen influences on oxytocin mRNA expression in preoptic and anterior hypothalamic regions studied by in situ hybridization
Authors
Keywordsbehavioral control
hypothalamus
immunocytochemistry
neuroendrocine control
Issue Date1991
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 1991, v. 307 n. 2, p. 281-295 How to Cite?
AbstractPossible estrogen influences on oxytocin mRNA expression were studied in preoptic and anterior hypothalamic regions as might be important for behavioral as well as neuroendocrine controls. In situ hybridization for oxytocin mRNA determination was supported by immunocytochemical identification and was compared with vasopressin mRNA in situ hybridization. With these techniques, oxytocin-expressing neurons were identified in medical preoptic, anterior commissural, periventricular, paraventricular, supraoptic, and perifornical nuclei as well as the bed nucleus of the stria terminalis and intersupraopticoparaventricular (internuclear) islands. Distribution and number of oxytocin mRNA-containing neurons and oxytocin mRNA levels were compared between ovariectomized control rats given cholesterol implants and ovariectomized rats given short-term (2 days) or long-term (2 months) estradiol treatment (10% estradiol, subcutaneous silastic implants). Effectiveness of long-term estrogen treatment was confirmed behaviorally. While there was a trend in several cell groups for a larger number of oxytocin-mRNA-containing neurons to be observed following 2 days of estrogen treatment, this was not statistically significant. Moreover, additional oxytocin-mRNA containing cell groups were not seen after short or long estradiol treatment. With computer-aided analysis, mean pixels per oxytocin-mRNA expressing neuron (reflecting oxytocin mRNA content) were compared between groups: In the supraoptic nucleus and the anterior commissural nucleus, these were increased both by 2 days and 2 months of estradiol treatment. These differences may be important in modulating female reproductive behavior. Present findings also suggest that estradiol can affect the oxytocinergic system via an indirect route since the cell groups influenced here by estradiol do not contain estrogen receptors. Oxytocinergic neurons may serve as a good system to compare direct transcriptional with indirect effects of hormones.
Persistent Identifierhttp://hdl.handle.net/10722/146616
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChung, SKen_HK
dc.contributor.authorMcCabe, JTen_HK
dc.contributor.authorPfaff, DWen_HK
dc.date.accessioned2012-05-08T03:21:17Z-
dc.date.available2012-05-08T03:21:17Z-
dc.date.issued1991en_HK
dc.identifier.citationJournal Of Comparative Neurology, 1991, v. 307 n. 2, p. 281-295en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146616-
dc.description.abstractPossible estrogen influences on oxytocin mRNA expression were studied in preoptic and anterior hypothalamic regions as might be important for behavioral as well as neuroendocrine controls. In situ hybridization for oxytocin mRNA determination was supported by immunocytochemical identification and was compared with vasopressin mRNA in situ hybridization. With these techniques, oxytocin-expressing neurons were identified in medical preoptic, anterior commissural, periventricular, paraventricular, supraoptic, and perifornical nuclei as well as the bed nucleus of the stria terminalis and intersupraopticoparaventricular (internuclear) islands. Distribution and number of oxytocin mRNA-containing neurons and oxytocin mRNA levels were compared between ovariectomized control rats given cholesterol implants and ovariectomized rats given short-term (2 days) or long-term (2 months) estradiol treatment (10% estradiol, subcutaneous silastic implants). Effectiveness of long-term estrogen treatment was confirmed behaviorally. While there was a trend in several cell groups for a larger number of oxytocin-mRNA-containing neurons to be observed following 2 days of estrogen treatment, this was not statistically significant. Moreover, additional oxytocin-mRNA containing cell groups were not seen after short or long estradiol treatment. With computer-aided analysis, mean pixels per oxytocin-mRNA expressing neuron (reflecting oxytocin mRNA content) were compared between groups: In the supraoptic nucleus and the anterior commissural nucleus, these were increased both by 2 days and 2 months of estradiol treatment. These differences may be important in modulating female reproductive behavior. Present findings also suggest that estradiol can affect the oxytocinergic system via an indirect route since the cell groups influenced here by estradiol do not contain estrogen receptors. Oxytocinergic neurons may serve as a good system to compare direct transcriptional with indirect effects of hormones.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subjectbehavioral controlen_HK
dc.subjecthypothalamusen_HK
dc.subjectimmunocytochemistryen_HK
dc.subjectneuroendrocine controlen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCholesterol - Metabolismen_US
dc.subject.meshEstrogens - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHypothalamus, Anterior - Drug Effects - Metabolismen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshOvariectomyen_US
dc.subject.meshOxytocin - Biosynthesisen_US
dc.subject.meshParaventricular Hypothalamic Nucleus - Metabolismen_US
dc.subject.meshPreoptic Area - Drug Effects - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSupraoptic Nucleus - Cytology - Drug Effects - Metabolismen_US
dc.titleEstrogen influences on oxytocin mRNA expression in preoptic and anterior hypothalamic regions studied by in situ hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1856326-
dc.identifier.scopuseid_2-s2.0-0025897305en_HK
dc.identifier.volume307en_HK
dc.identifier.issue2en_HK
dc.identifier.spage281en_HK
dc.identifier.epage295en_HK
dc.identifier.isiWOS:A1991FJ95600008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridMcCabe, JT=35516007000en_HK
dc.identifier.scopusauthoridPfaff, DW=36046651600en_HK

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