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Article: Immunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe

TitleImmunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe
Authors
Keywordsactin
colloidal gold
dendritic spine
immunocytochemistry
postsynaptic density
Issue Date1985
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340
Citation
Cellular And Molecular Neurobiology, 1985, v. 5 n. 3, p. 271-284 How to Cite?
Abstract1. Immunocytochemical localization of actin in rat cerebral cortex embedded in the resin LR White was performed using 5 nm colloidal gold as a probe. Antigenicity is maintained throughout the embedding procedure and the low electron opacity of LR White permits fine filamentous structures to be visualized. Control experiments included incubating the sections with normal goat serum or mouse IgG instead of the primary antibody, preadsorbing the antibody with actin from bovine muscle or liver acetone powder, and heat treating the primary antibody. 2. Immunoreactive actin was identified primarily in dendritic spines, particularly in the postsynaptic density (PSD), the subsynaptic web, and the spine apparatus and endothelial and smooth muscle cells of blood vessels. 3. Within dendritic spines, actin which is labeled in the PSD is in continuity with the filaments of the subsynaptic web. These filaments, in turn, are in continuity with the spine apparatus and/or the spine membranes adjacent to the PSD. The PSD may therefore function like other submembranous filamentous arrays which communicate events occurring at the membrane, in this case, the postsynaptic membrane, to the underlying cytoskeletal network, i.e., the subsynaptic web of the spine. It is also suggested that the actin present in the spine may play a role in changes in spine shape and synaptic curvature. Some actin was also seen in the presynaptic process in association with synaptic vesicles, the filamentous network that is contiguous with the synaptic vesicle membrane, and the presynaptic dense projections. Actin may be involved in dynamic processes in the presynaptic ending which include vesicle translocation. © 1985 Plenum Publishing Corporation.
Persistent Identifierhttp://hdl.handle.net/10722/146611
ISSN
2015 Impact Factor: 2.328
2015 SCImago Journal Rankings: 1.005
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCohen, RSen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorPfaff, DWen_HK
dc.date.accessioned2012-05-08T03:21:15Z-
dc.date.available2012-05-08T03:21:15Z-
dc.date.issued1985en_HK
dc.identifier.citationCellular And Molecular Neurobiology, 1985, v. 5 n. 3, p. 271-284en_HK
dc.identifier.issn0272-4340en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146611-
dc.description.abstract1. Immunocytochemical localization of actin in rat cerebral cortex embedded in the resin LR White was performed using 5 nm colloidal gold as a probe. Antigenicity is maintained throughout the embedding procedure and the low electron opacity of LR White permits fine filamentous structures to be visualized. Control experiments included incubating the sections with normal goat serum or mouse IgG instead of the primary antibody, preadsorbing the antibody with actin from bovine muscle or liver acetone powder, and heat treating the primary antibody. 2. Immunoreactive actin was identified primarily in dendritic spines, particularly in the postsynaptic density (PSD), the subsynaptic web, and the spine apparatus and endothelial and smooth muscle cells of blood vessels. 3. Within dendritic spines, actin which is labeled in the PSD is in continuity with the filaments of the subsynaptic web. These filaments, in turn, are in continuity with the spine apparatus and/or the spine membranes adjacent to the PSD. The PSD may therefore function like other submembranous filamentous arrays which communicate events occurring at the membrane, in this case, the postsynaptic membrane, to the underlying cytoskeletal network, i.e., the subsynaptic web of the spine. It is also suggested that the actin present in the spine may play a role in changes in spine shape and synaptic curvature. Some actin was also seen in the presynaptic process in association with synaptic vesicles, the filamentous network that is contiguous with the synaptic vesicle membrane, and the presynaptic dense projections. Actin may be involved in dynamic processes in the presynaptic ending which include vesicle translocation. © 1985 Plenum Publishing Corporation.en_HK
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340en_HK
dc.relation.ispartofCellular and Molecular Neurobiologyen_HK
dc.subjectactinen_HK
dc.subjectcolloidal golden_HK
dc.subjectdendritic spineen_HK
dc.subjectimmunocytochemistryen_HK
dc.subjectpostsynaptic densityen_HK
dc.subject.meshActins - Analysisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCerebral Cortex - Ultrastructureen_US
dc.subject.meshDendrites - Ultrastructureen_US
dc.subject.meshFemaleen_US
dc.subject.meshGolden_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshImmunochemistryen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshNeurons - Ultrastructureen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshSynapses - Ultrastructureen_US
dc.titleImmunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probeen_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00711012en_HK
dc.identifier.pmid4064076-
dc.identifier.scopuseid_2-s2.0-0022119267en_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.spage271en_HK
dc.identifier.epage284en_HK
dc.identifier.isiWOS:A1985ASN3200006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCohen, RS=35560739900en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridPfaff, DW=36046651600en_HK

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