File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Transgenic mice with overexpression of human scavenger receptor A on endothelial cells.

TitleTransgenic mice with overexpression of human scavenger receptor A on endothelial cells.
Authors
Issue Date2001
PublisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2001, v. 114 n. 10, p. 1078-1083 How to Cite?
AbstractOBJECTIVES: To establish a new transgenic mouse model for determining the function and role of human scavenger receptor A (SR-A) in atherosclerosis in vivo. METHODS: Human scavenger receptor minigene-driven mouse tie-1 promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via the microinjection method. PCR and Southern blot were used to screen the positive transgenic mice. RT-PCR and immunohistochemical analysis were used to detect the level and location of human SR-AI expression in transgenic mice. The activity of human SR-AI was determined by morphologic observation of aortic endothelial cells of transgenic mice under transmission electron microscopy. RESULTS: The electrophoresis assay showed the expected 4 fragments of 0.9 kb, 1.1 kb, 1.2 kb and 4.2 kb in the Sma I digest and 2 fragments of 0.8 kb and 6.7 kb in Bgl II digest of plasmids pTie-1/hSR-A. The fragment sequence of tie-1 promoter and human SR-A cDNA in plasmids pTie-1/hSR-A was correct and no ATG before the translation initiation sites of human SR-A was found by sequence analysis. 561 injected and surviving embryos with the purified human SR-A minigene were implanted into the oviducts of 19 ICR pseudopregnant mice. Among the 54 surviving pups from 13 foster mothers, 7 were identified by PCR and Southern blot analysis. The results of RT-PCR and immunohistochemical analysis showed human SR-A was specifically expressed on vascular endothelial cells of the aorta and renal artery, as well as hepatic sinusoidal endothelial cells in transgenic mice. Transmission electron microscope (TEM) of aorta of transgenic mice showed that a large number of vesicles, multivesicle bodies and swollen mitochondria filled the plasma of endothelial cells. CONCLUSIONS: A transgenic mouse model with overexpression of human SR-A in endothelial cells was successfully established. The transgene was integrated and transmitted into the chromosome of transgenic mice. Tie-1 promoter controlled the transgene to express in endothelial cells in mice. Pinocytic activity of aortic endothelial cells in transgenic mice was higher than that of C57BL/6J mice. Our studies will provide a new transgenic model for investigation of atherosclerosis and functions of human SR-A.
Persistent Identifierhttp://hdl.handle.net/10722/146608
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWan, Len_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorYang, Yen_HK
dc.contributor.authorChung, SSen_HK
dc.contributor.authorCao, Den_HK
dc.contributor.authorMa, Men_HK
dc.contributor.authorWu, Men_HK
dc.contributor.authorWan, Zen_HK
dc.contributor.authorChen, Xen_HK
dc.date.accessioned2012-05-08T03:21:14Z-
dc.date.available2012-05-08T03:21:14Z-
dc.date.issued2001en_HK
dc.identifier.citationChinese Medical Journal, 2001, v. 114 n. 10, p. 1078-1083en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146608-
dc.description.abstractOBJECTIVES: To establish a new transgenic mouse model for determining the function and role of human scavenger receptor A (SR-A) in atherosclerosis in vivo. METHODS: Human scavenger receptor minigene-driven mouse tie-1 promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via the microinjection method. PCR and Southern blot were used to screen the positive transgenic mice. RT-PCR and immunohistochemical analysis were used to detect the level and location of human SR-AI expression in transgenic mice. The activity of human SR-AI was determined by morphologic observation of aortic endothelial cells of transgenic mice under transmission electron microscopy. RESULTS: The electrophoresis assay showed the expected 4 fragments of 0.9 kb, 1.1 kb, 1.2 kb and 4.2 kb in the Sma I digest and 2 fragments of 0.8 kb and 6.7 kb in Bgl II digest of plasmids pTie-1/hSR-A. The fragment sequence of tie-1 promoter and human SR-A cDNA in plasmids pTie-1/hSR-A was correct and no ATG before the translation initiation sites of human SR-A was found by sequence analysis. 561 injected and surviving embryos with the purified human SR-A minigene were implanted into the oviducts of 19 ICR pseudopregnant mice. Among the 54 surviving pups from 13 foster mothers, 7 were identified by PCR and Southern blot analysis. The results of RT-PCR and immunohistochemical analysis showed human SR-A was specifically expressed on vascular endothelial cells of the aorta and renal artery, as well as hepatic sinusoidal endothelial cells in transgenic mice. Transmission electron microscope (TEM) of aorta of transgenic mice showed that a large number of vesicles, multivesicle bodies and swollen mitochondria filled the plasma of endothelial cells. CONCLUSIONS: A transgenic mouse model with overexpression of human SR-A in endothelial cells was successfully established. The transgene was integrated and transmitted into the chromosome of transgenic mice. Tie-1 promoter controlled the transgene to express in endothelial cells in mice. Pinocytic activity of aortic endothelial cells in transgenic mice was higher than that of C57BL/6J mice. Our studies will provide a new transgenic model for investigation of atherosclerosis and functions of human SR-A.en_HK
dc.languageengen_US
dc.publisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese medical journalen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshArteriosclerosis - Etiologyen_US
dc.subject.meshEndothelial Cells - Metabolism - Ultrastructureen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Inbred Cbaen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshReceptors, Immunologic - Genetics - Physiologyen_US
dc.subject.meshScavenger Receptors, Class Aen_US
dc.titleTransgenic mice with overexpression of human scavenger receptor A on endothelial cells.en_HK
dc.typeArticleen_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SS: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityChung, SS=rp00376en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid11677771-
dc.identifier.scopuseid_2-s2.0-0013022741en_HK
dc.identifier.hkuros69269-
dc.identifier.volume114en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1078en_HK
dc.identifier.epage1083en_HK
dc.identifier.isiWOS:000171580500017-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridWan, L=12788141200en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridYang, Y=7409385808en_HK
dc.identifier.scopusauthoridChung, SS=14120761600en_HK
dc.identifier.scopusauthoridCao, D=16645038400en_HK
dc.identifier.scopusauthoridMa, M=26659963300en_HK
dc.identifier.scopusauthoridWu, M=37262258900en_HK
dc.identifier.scopusauthoridWan, Z=7101835871en_HK
dc.identifier.scopusauthoridChen, X=8862255600en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats