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Article: miR-135a regulates preimplantation embryo development through down-regulation of E3 ubiquitin ligase seven in absentia homolog 1A (SIAH1A) expression

TitlemiR-135a regulates preimplantation embryo development through down-regulation of E3 ubiquitin ligase seven in absentia homolog 1A (SIAH1A) expression
Authors
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2011, v. 6 n. 11 How to Cite?
AbstractBackground: MicroRNAs (miRNAs) are small non-coding RNA molecules capable of regulating transcription and translation. Previously, a cluster of miRNAs that are specifically expressed in mouse zygotes but not in oocytes or other preimplantation stages embryos are identified by multiplex real-time polymerase chain reaction-based miRNA profiling. The functional role of one of these zygote-specific miRNAs, miR-135a, in preimplantation embryo development was investigated. Methodology/Principal Findings: Microinjection of miR-135a inhibitor suppressed first cell cleavage in more than 30% of the zygotes. Bioinformatics analysis identified E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (Siah1a) as a predicted target of miR-135a. Western blotting and 3′UTR luciferase functional assays demonstrated that miR-135a down-regulated the expression of Siah1 in HeLa cells and in mouse zygotes. Siah1a was expressed in preimplantation embryos and its expression pattern negatively correlated with that of miR-135a. Co-injection of Siah1a-specific antibody with miR-135a inhibitor partially nullified the effect of miR-135a inhibition. Proteasome inhibition by MG-132 revealed that miR-135a regulated proteasomal degradation and potentially controlled the expression of chemokinesin DNA binding protein (Kid). Conclusions/Significance: The present study demonstrated for the first time that zygotic specific miRNA modulates the first cell cleavage through regulating expression of Siah1a. © 2011 Pang et al.
Persistent Identifierhttp://hdl.handle.net/10722/146430
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Department of Obstetrics and Gynaecology, The University of Hong Kong
Funding Information:

This work was supported by the general account from the Department of Obstetrics and Gynaecology, The University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorLiu, WMen_HK
dc.contributor.authorLeung, CONen_HK
dc.contributor.authorYe, TMen_HK
dc.contributor.authorKwan, PCKen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-04-24T07:53:38Z-
dc.date.available2012-04-24T07:53:38Z-
dc.date.issued2011en_HK
dc.identifier.citationPlos One, 2011, v. 6 n. 11en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146430-
dc.description.abstractBackground: MicroRNAs (miRNAs) are small non-coding RNA molecules capable of regulating transcription and translation. Previously, a cluster of miRNAs that are specifically expressed in mouse zygotes but not in oocytes or other preimplantation stages embryos are identified by multiplex real-time polymerase chain reaction-based miRNA profiling. The functional role of one of these zygote-specific miRNAs, miR-135a, in preimplantation embryo development was investigated. Methodology/Principal Findings: Microinjection of miR-135a inhibitor suppressed first cell cleavage in more than 30% of the zygotes. Bioinformatics analysis identified E3 Ubiquitin Ligase Seven In Absentia Homolog 1A (Siah1a) as a predicted target of miR-135a. Western blotting and 3′UTR luciferase functional assays demonstrated that miR-135a down-regulated the expression of Siah1 in HeLa cells and in mouse zygotes. Siah1a was expressed in preimplantation embryos and its expression pattern negatively correlated with that of miR-135a. Co-injection of Siah1a-specific antibody with miR-135a inhibitor partially nullified the effect of miR-135a inhibition. Proteasome inhibition by MG-132 revealed that miR-135a regulated proteasomal degradation and potentially controlled the expression of chemokinesin DNA binding protein (Kid). Conclusions/Significance: The present study demonstrated for the first time that zygotic specific miRNA modulates the first cell cleavage through regulating expression of Siah1a. © 2011 Pang et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshDown-Regulation - genetics-
dc.subject.meshEmbryonic Development - genetics-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshMicroRNAs - antagonists and inhibitors - genetics - metabolism-
dc.subject.meshUbiquitin-Protein Ligases - genetics - metabolism-
dc.titlemiR-135a regulates preimplantation embryo development through down-regulation of E3 ubiquitin ligase seven in absentia homolog 1A (SIAH1A) expressionen_HK
dc.typeArticleen_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0027878en_HK
dc.identifier.pmid22132158-
dc.identifier.pmcidPMC3222661-
dc.identifier.scopuseid_2-s2.0-81555212311en_HK
dc.identifier.hkuros199373en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-81555212311&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue11en_HK
dc.identifier.isiWOS:000297792400020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridLiu, WM=54682064800en_HK
dc.identifier.scopusauthoridLeung, CON=36140510700en_HK
dc.identifier.scopusauthoridYe, TM=36166071700en_HK
dc.identifier.scopusauthoridKwan, PCK=54681950200en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=55763794905en_HK

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