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Article: The biomechanics of drug-treated leukemia cells investigated using optical tweezers

TitleThe biomechanics of drug-treated leukemia cells investigated using optical tweezers
Authors
KeywordsOptical tweezers
Indentation
Leukemia cells
Cell mechanics
Issue Date2012
PublisherWorld Scientific Publishing Co. Pte. Ltd. The Journal's web site is located at http://www.worldscinet.com/nl/nl.shtml
Citation
Nano Life, 2012, v. 2 n. 1, article no. 1250010 How to Cite?
AbstractLeukemia is a very common cancer worldwide, and different drugs have been applied to treat the disease. However, the influence of the drugs on the biomechanical properties of leukemia cells, which are related to the risk of leukostasis, is still unknown. Moreover, accurate measurement of biomechanical properties of leukemia cells is still a challenging task because of their non-adherent nature and high sensitivity to the surrounding physiological conditions. In this study, a protocol to measure the biomechanical properties of leukemia cells by performing indentation tests using optical tweezers is proposed. The biomechanical properties of normal leukemia cells and cells treated with various cancer drugs, including phorbol 12-myristate 13-acetate (PMA), all-trans retinoic acid (ATRa), Cytoxan (CTX), and Dexamethasone (DEX), were measured. The adhesion between the cells and certain proteins existing in the extracellular matrix, i.e., fibronetin and collagen I, was also characterized with the help of a static adhesion assay. It was found that after treatment by ATRa, CTX, and DEX, the cells became softer, and the adhesion between the cells and the proteins became weaker. PMA treatment caused no change in the stiffness of the HL60 cells, but increased the stiffness of the K562 cells, and increased the cell–protein adhesion of both K562 cells and HL60 cells.
Persistent Identifierhttp://hdl.handle.net/10722/146399
ISSN

 

DC FieldValueLanguage
dc.contributor.authorZhou, ZLen_US
dc.contributor.authorTang, Ben_US
dc.contributor.authorNgan, AHWen_US
dc.date.accessioned2012-04-24T07:51:37Z-
dc.date.available2012-04-24T07:51:37Z-
dc.date.issued2012en_US
dc.identifier.citationNano Life, 2012, v. 2 n. 1, article no. 1250010en_US
dc.identifier.issn1793-9844-
dc.identifier.urihttp://hdl.handle.net/10722/146399-
dc.description.abstractLeukemia is a very common cancer worldwide, and different drugs have been applied to treat the disease. However, the influence of the drugs on the biomechanical properties of leukemia cells, which are related to the risk of leukostasis, is still unknown. Moreover, accurate measurement of biomechanical properties of leukemia cells is still a challenging task because of their non-adherent nature and high sensitivity to the surrounding physiological conditions. In this study, a protocol to measure the biomechanical properties of leukemia cells by performing indentation tests using optical tweezers is proposed. The biomechanical properties of normal leukemia cells and cells treated with various cancer drugs, including phorbol 12-myristate 13-acetate (PMA), all-trans retinoic acid (ATRa), Cytoxan (CTX), and Dexamethasone (DEX), were measured. The adhesion between the cells and certain proteins existing in the extracellular matrix, i.e., fibronetin and collagen I, was also characterized with the help of a static adhesion assay. It was found that after treatment by ATRa, CTX, and DEX, the cells became softer, and the adhesion between the cells and the proteins became weaker. PMA treatment caused no change in the stiffness of the HL60 cells, but increased the stiffness of the K562 cells, and increased the cell–protein adhesion of both K562 cells and HL60 cells.-
dc.languageengen_US
dc.publisherWorld Scientific Publishing Co. Pte. Ltd. The Journal's web site is located at http://www.worldscinet.com/nl/nl.shtmlen_US
dc.relation.ispartofNano Lifeen_US
dc.rightsNano Life. Copyright © World Scientific Publishing Co. Pte. Ltd.-
dc.rightsElectronic version of an article published as Nano Life, 2012, v. 2 n. 1, article no. 1250010. DOI: 10.1142/S179398441100044X-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectOptical tweezers-
dc.subjectIndentation-
dc.subjectLeukemia cells-
dc.subjectCell mechanics-
dc.titleThe biomechanics of drug-treated leukemia cells investigated using optical tweezersen_US
dc.typeArticleen_US
dc.identifier.emailTang, B: tangbin@hkucc.hku.hken_US
dc.identifier.emailNgan, AHW: hwngan@hkucc.hku.hk-
dc.identifier.authorityNgan, AHW=rp00225en_US
dc.description.naturepostprint-
dc.identifier.doi10.1142/S179398441100044X-
dc.identifier.hkuros199355en_US
dc.identifier.volume2en_US
dc.identifier.issue1, article no. 1250010en_US
dc.publisher.placeSingapore-

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