File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of human metapneumoviruses isolated in Chongqing, China

TitleCharacterization of human metapneumoviruses isolated in Chongqing, China
Authors
KeywordsChina
Isolation
Metapneumovirus
Phylogeny
Issue Date2008
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2008, v. 121 n. 22, p. 2254-2257 How to Cite?
AbstractBackground: Human metapneumovirus (hMPV) has recently been recognized as a notable respiratory pathogen in children. However, no isolation processes and only a limited understanding of hMPV epidemiology present in Chinese children are documented by far. Methods: Nasopharyngeal aspirates from 86 hospitalized children with acute respiratory tract infections from December 2004 to July 2005 were inoculated onto Vero-E6 cells for hMPV isolation. Total RNA was extracted from infected cells with a cytopathic effect and then subjected to a RT-PCR amplification of the N and F genes of the hMPV. Nucleotide sequences of amplified F gene products were examined using a variation analysis with the MegAlign program and the phylogenetic tree construction using the neighbor-joining algorithm with a Phylip package. Results: Six strains of hMPV were isolated from the samples during winter, spring and summer. The most common symptoms were coughing and cyanosis, and the diagnoses were bronchiolitis, bronchopneumonia, infantile asthma, or upper respiratory tract infection. All isolates were in the A2 genetic sublineage and shared a high percentage of homology with the F gene in the nucleotide (99.8%-100%) and amino acid (99.3%-100%) sequences. Conclusions: This report indicates that hMPV is an important viral agent for acute respiratory tract infections present in Chongqing, China. Knowledge of phylogeny and genes will benefit the studies on the treatment and prophylaxis of hMPV infection.
Persistent Identifierhttp://hdl.handle.net/10722/146341
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMao, HWen_HK
dc.contributor.authorYang, XQen_HK
dc.contributor.authorZhao, XDen_HK
dc.date.accessioned2012-04-20T02:05:09Z-
dc.date.available2012-04-20T02:05:09Z-
dc.date.issued2008en_HK
dc.identifier.citationChinese Medical Journal, 2008, v. 121 n. 22, p. 2254-2257en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146341-
dc.description.abstractBackground: Human metapneumovirus (hMPV) has recently been recognized as a notable respiratory pathogen in children. However, no isolation processes and only a limited understanding of hMPV epidemiology present in Chinese children are documented by far. Methods: Nasopharyngeal aspirates from 86 hospitalized children with acute respiratory tract infections from December 2004 to July 2005 were inoculated onto Vero-E6 cells for hMPV isolation. Total RNA was extracted from infected cells with a cytopathic effect and then subjected to a RT-PCR amplification of the N and F genes of the hMPV. Nucleotide sequences of amplified F gene products were examined using a variation analysis with the MegAlign program and the phylogenetic tree construction using the neighbor-joining algorithm with a Phylip package. Results: Six strains of hMPV were isolated from the samples during winter, spring and summer. The most common symptoms were coughing and cyanosis, and the diagnoses were bronchiolitis, bronchopneumonia, infantile asthma, or upper respiratory tract infection. All isolates were in the A2 genetic sublineage and shared a high percentage of homology with the F gene in the nucleotide (99.8%-100%) and amino acid (99.3%-100%) sequences. Conclusions: This report indicates that hMPV is an important viral agent for acute respiratory tract infections present in Chongqing, China. Knowledge of phylogeny and genes will benefit the studies on the treatment and prophylaxis of hMPV infection.en_HK
dc.languageengen_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese Medical Journalen_HK
dc.subjectChinaen_HK
dc.subjectIsolationen_HK
dc.subjectMetapneumovirusen_HK
dc.subjectPhylogenyen_HK
dc.titleCharacterization of human metapneumoviruses isolated in Chongqing, Chinaen_HK
dc.typeArticleen_HK
dc.identifier.emailMao, HW:hwmau@hku.hken_HK
dc.identifier.authorityMao, HW=rp01595en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid19080329-
dc.identifier.scopuseid_2-s2.0-62749173534en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62749173534&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume121en_HK
dc.identifier.issue22en_HK
dc.identifier.spage2254en_HK
dc.identifier.epage2257en_HK
dc.identifier.isiWOS:000261364200009-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridMao, HW=25632489000en_HK
dc.identifier.scopusauthoridYang, XQ=13606095400en_HK
dc.identifier.scopusauthoridZhao, XD=7407577513en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats