Article: Lutein enhances survival and reduces neuronal damage in a mouse model of ischemic stroke
| Title | Lutein enhances survival and reduces neuronal damage in a mouse model of ischemic stroke | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Li, SY1 Yang, D1 Fu, ZJ1 Woo, T1 Wong, D1 2 Lo, ACY1 | ||||||
| Keywords | Apoptosis Cerebral infarct Inflammation Ischemic stroke Oxidative stress Xanthophylls | ||||||
| Issue Date | 2012 | ||||||
| Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynbdi | ||||||
| Citation | Neurobiology Of Disease, 2012, v. 45 n. 1, p. 624-632 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.nbd.2011.10.008 | ||||||
| Abstract | Introduction: Stroke is one of the leading causes of death worldwide. Protective agents that could diminish the injuries induced by cerebral ischemia/reperfusion (I/R) are crucial to alleviate the detrimental outcome of stroke. The aim of this study is to investigate the protective roles of lutein in cerebral I/R injury. Methods: Two-hour cerebral ischemia was induced by unilateral middle cerebral artery occlusion (MCAo) in mice. Either lutein (0.2. mg/kg) or vehicle was given to mice intraperitoneally 1. h after MCAo and 1. h after reperfusion. Neurological deficits were evaluated at 22. h after reperfusion while survival rate was assessed daily until 7. days after reperfusion. Brains were cut into 2. mm-thick coronal slices and stained with 2% 2,3,5-triphenyltetrazolium chloride to determine the infarct size after MCAo. Paraffin-embedded brain sections were prepared for TUNEL assay and immunohistochemistry. Protein lysate was collected for Western blotting experiments. Results: Higher survival rate, better neurological scores, smaller infarct area and smaller infarct volume were noted in the lutein-treated group. Immunohistochemistry data showed a decrease of immunoreactivity of nitrotyrosine, poly(ADP-ribose) and NFκB in the lutein-treated brains. Western blotting data showed decreased levels of Cox-2, pERK, and pIκB, but increased levels of Bcl-2, heat shock protein 70 and pAkt in the lutein-treated brains. Conclusions: Post-treatment of lutein protected the brain from I/R injury, probably by its anti-apoptotic, anti-oxidative and anti-inflammatory properties. These suggest that lutein could diminish the deleterious outcomes of cerebral I/R and may be used as a potential treatment for stroke patients. © 2011 Elsevier Inc.. | ||||||
| ISSN | 0969-9961 2011 Impact Factor: 5.403 2011 SCImago Journal Rankings: 0.506 | ||||||
| DOI | http://dx.doi.org/10.1016/j.nbd.2011.10.008 | ||||||
| ISI Accession Number ID | WOS:000297883500069
Funding Information: This research was supported by the grants from the Hong Kong Research Grants Council (GRF #HKU773210M) and the University Development Fund from The University of Hong Kong. | ||||||
| References | References in Scopus |
| dc.contributor.author | Li, SY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Yang, D | ||||||
| dc.contributor.author | Fu, ZJ | ||||||
| dc.contributor.author | Woo, T | ||||||
| dc.contributor.author | Wong, D | ||||||
| dc.contributor.author | Lo, ACY | ||||||
| dc.date.accessioned | 2012-04-10T01:50:11Z | ||||||
| dc.date.available | 2012-04-10T01:50:11Z | ||||||
| dc.date.issued | 2012 | ||||||
| dc.description.abstract | Introduction: Stroke is one of the leading causes of death worldwide. Protective agents that could diminish the injuries induced by cerebral ischemia/reperfusion (I/R) are crucial to alleviate the detrimental outcome of stroke. The aim of this study is to investigate the protective roles of lutein in cerebral I/R injury. Methods: Two-hour cerebral ischemia was induced by unilateral middle cerebral artery occlusion (MCAo) in mice. Either lutein (0.2. mg/kg) or vehicle was given to mice intraperitoneally 1. h after MCAo and 1. h after reperfusion. Neurological deficits were evaluated at 22. h after reperfusion while survival rate was assessed daily until 7. days after reperfusion. Brains were cut into 2. mm-thick coronal slices and stained with 2% 2,3,5-triphenyltetrazolium chloride to determine the infarct size after MCAo. Paraffin-embedded brain sections were prepared for TUNEL assay and immunohistochemistry. Protein lysate was collected for Western blotting experiments. Results: Higher survival rate, better neurological scores, smaller infarct area and smaller infarct volume were noted in the lutein-treated group. Immunohistochemistry data showed a decrease of immunoreactivity of nitrotyrosine, poly(ADP-ribose) and NFκB in the lutein-treated brains. Western blotting data showed decreased levels of Cox-2, pERK, and pIκB, but increased levels of Bcl-2, heat shock protein 70 and pAkt in the lutein-treated brains. Conclusions: Post-treatment of lutein protected the brain from I/R injury, probably by its anti-apoptotic, anti-oxidative and anti-inflammatory properties. These suggest that lutein could diminish the deleterious outcomes of cerebral I/R and may be used as a potential treatment for stroke patients. © 2011 Elsevier Inc.. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Neurobiology Of Disease, 2012, v. 45 n. 1, p. 624-632 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.nbd.2011.10.008 | ||||||
| dc.identifier.citeulike | 9932106 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.nbd.2011.10.008 | ||||||
| dc.identifier.epage | 632 | ||||||
| dc.identifier.hkuros | 205718 | ||||||
| dc.identifier.isi | WOS:000297883500069
Funding Information: This research was supported by the grants from the Hong Kong Research Grants Council (GRF #HKU773210M) and the University Development Fund from The University of Hong Kong. | ||||||
| dc.identifier.issn | 0969-9961 2011 Impact Factor: 5.403 2011 SCImago Journal Rankings: 0.506 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.pmid | 22024715 | ||||||
| dc.identifier.scopus | eid_2-s2.0-81955167409 | ||||||
| dc.identifier.spage | 624 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/146317 | ||||||
| dc.identifier.volume | 45 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynbdi | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Neurobiology of Disease | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Apoptosis | ||||||
| dc.subject | Cerebral infarct | ||||||
| dc.subject | Inflammation | ||||||
| dc.subject | Ischemic stroke | ||||||
| dc.subject | Oxidative stress | ||||||
| dc.subject | Xanthophylls | ||||||
| dc.title | Lutein enhances survival and reduces neuronal damage in a mouse model of ischemic stroke | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- University of Liverpool