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Article: Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?

TitleDoes the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?
Authors
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ophtha
Citation
Ophthalmology, 2010, v. 117 n. 2, p. 320-323.e1 How to Cite?
AbstractPurpose: To determine whether the presence of a clinically and/or microscopically detectable epiretinal membrane (ERM) alters the cleavage plane during internal limiting membrane (ILM) peeling. Design: Retrospective, observational, immunohistochemical study of ILM specimens using archival formalin-fixed, paraffin-embedded tissue. Participants: Fifty-one patients who had had ILM excision. Methods: Fifty-one ILM specimens peeled during vitrectomy for various etiologies were examined by light microscopy. The removal of ILM was assisted using Trypan blue (n = 30), indocyanine green (n = 7), or brilliant blue G (n = 14). Monoclonal antibodies to glial fibrillary acidic protein and to neurofilament protein were used to detect glial or neuronal cells respectively on the vitreous or retinal surfaces of the ILM. Specimens were divided into 2 groups: ILM peeled for full-thickness macular hole (MH; n = 31) and ILM peeled after removal of clinically detectable ERM (n = 20). Main Outcome Measures: Primary outcome measure was the localization of immunohistochemical markers to neuronal or glial cells on the vitreous or retinal surfaces of ILM. The secondary outcome measure was the correlation of the results of the primary measure with the dyes used to facilitate ILM peeling. Results: Glial and/or neuronal cells were detected on the retinal surface of the ILM in 10 of 31 (32%) of the MH ILM specimens and in 13 of 20 (65%) of the ILM peeled after ERM excision; the difference was significant (P = 0.02). There was no association between the presence of neuronal and glial cells with the type of dye used (P = 0.2). Of the 23 ILM specimens with cells attached to the retinal surface, 21 (91%) were associated with clinical and/or histologic evidence of ERM and 2 (9%) were not. The correlation between the presence of cells on the vitreous and the retinal surfaces of ILM was high (P<0.0001). Conclusions: The findings suggest that ERM may be associated with sub-ILM changes that alter the plane of separation during ILM peeling. This study does not confirm any influence of dyes on the cleavage plane during surgery. Financial Disclosure(s): The authors have no proprietary or commercial interest in any of the materials discussed in this article. © 2010 American Academy of Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/146309
ISSN
2015 Impact Factor: 6.75
2015 SCImago Journal Rankings: 4.745
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKenawy, Nen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorStappler, Ten_HK
dc.contributor.authorRomano, MRen_HK
dc.contributor.authorDas, RAen_HK
dc.contributor.authorHebbar, Gen_HK
dc.contributor.authorPrime, Wen_HK
dc.contributor.authorHeimann, Hen_HK
dc.contributor.authorGibran, SKen_HK
dc.contributor.authorSheridan, CMen_HK
dc.contributor.authorCheung, YHen_HK
dc.contributor.authorHiscott, PSen_HK
dc.date.accessioned2012-04-10T01:50:07Z-
dc.date.available2012-04-10T01:50:07Z-
dc.date.issued2010en_HK
dc.identifier.citationOphthalmology, 2010, v. 117 n. 2, p. 320-323.e1en_HK
dc.identifier.issn0161-6420en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146309-
dc.description.abstractPurpose: To determine whether the presence of a clinically and/or microscopically detectable epiretinal membrane (ERM) alters the cleavage plane during internal limiting membrane (ILM) peeling. Design: Retrospective, observational, immunohistochemical study of ILM specimens using archival formalin-fixed, paraffin-embedded tissue. Participants: Fifty-one patients who had had ILM excision. Methods: Fifty-one ILM specimens peeled during vitrectomy for various etiologies were examined by light microscopy. The removal of ILM was assisted using Trypan blue (n = 30), indocyanine green (n = 7), or brilliant blue G (n = 14). Monoclonal antibodies to glial fibrillary acidic protein and to neurofilament protein were used to detect glial or neuronal cells respectively on the vitreous or retinal surfaces of the ILM. Specimens were divided into 2 groups: ILM peeled for full-thickness macular hole (MH; n = 31) and ILM peeled after removal of clinically detectable ERM (n = 20). Main Outcome Measures: Primary outcome measure was the localization of immunohistochemical markers to neuronal or glial cells on the vitreous or retinal surfaces of ILM. The secondary outcome measure was the correlation of the results of the primary measure with the dyes used to facilitate ILM peeling. Results: Glial and/or neuronal cells were detected on the retinal surface of the ILM in 10 of 31 (32%) of the MH ILM specimens and in 13 of 20 (65%) of the ILM peeled after ERM excision; the difference was significant (P = 0.02). There was no association between the presence of neuronal and glial cells with the type of dye used (P = 0.2). Of the 23 ILM specimens with cells attached to the retinal surface, 21 (91%) were associated with clinical and/or histologic evidence of ERM and 2 (9%) were not. The correlation between the presence of cells on the vitreous and the retinal surfaces of ILM was high (P<0.0001). Conclusions: The findings suggest that ERM may be associated with sub-ILM changes that alter the plane of separation during ILM peeling. This study does not confirm any influence of dyes on the cleavage plane during surgery. Financial Disclosure(s): The authors have no proprietary or commercial interest in any of the materials discussed in this article. © 2010 American Academy of Ophthalmology.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ophthaen_HK
dc.relation.ispartofOphthalmologyen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshBasement Membrane - Metabolism - Pathology - Surgeryen_US
dc.subject.meshBenzenesulfonates - Diagnostic Useen_US
dc.subject.meshColoring Agents - Diagnostic Useen_US
dc.subject.meshEpiretinal Membrane - Diagnosisen_US
dc.subject.meshFemaleen_US
dc.subject.meshGlial Fibrillary Acidic Protein - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshIndocyanine Green - Diagnostic Useen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeurofilament Proteins - Metabolismen_US
dc.subject.meshNeuroglia - Pathologyen_US
dc.subject.meshNeurons - Pathologyen_US
dc.subject.meshRetinal Diseases - Surgeryen_US
dc.subject.meshRetrospective Studiesen_US
dc.subject.meshTrypan Blue - Diagnostic Useen_US
dc.subject.meshVitrectomyen_US
dc.titleDoes the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?en_HK
dc.typeArticleen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ophtha.2009.07.024en_HK
dc.identifier.pmid20006906-
dc.identifier.scopuseid_2-s2.0-75149153231en_HK
dc.identifier.hkuros181837-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75149153231&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume117en_HK
dc.identifier.issue2en_HK
dc.identifier.spage320en_HK
dc.identifier.epage323.e1en_HK
dc.identifier.isiWOS:000274530300019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKenawy, N=16318979300en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridStappler, T=8563727800en_HK
dc.identifier.scopusauthoridRomano, MR=35249070500en_HK
dc.identifier.scopusauthoridDas, RA=35344480900en_HK
dc.identifier.scopusauthoridHebbar, G=6602381679en_HK
dc.identifier.scopusauthoridPrime, W=6602358165en_HK
dc.identifier.scopusauthoridHeimann, H=7006780277en_HK
dc.identifier.scopusauthoridGibran, SK=13205333800en_HK
dc.identifier.scopusauthoridSheridan, CM=7004974390en_HK
dc.identifier.scopusauthoridCheung, YH=36105762600en_HK
dc.identifier.scopusauthoridHiscott, PS=7006368693en_HK
dc.identifier.citeulike6409003-

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