File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The presence of AC133-positive cells suggests a possible role of endothelial progenitor cells in the formation of choroidal neovascularization

TitleThe presence of AC133-positive cells suggests a possible role of endothelial progenitor cells in the formation of choroidal neovascularization
Authors
Issue Date2006
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2006, v. 47 n. 4, p. 1642-1645 How to Cite?
AbstractPURPOSE. Recent evidence suggests that vasculogenesis as well as angiogenesis occurs throughout the body during neovascularization. The recruitment of circulating stem cells is a key feature of vasculogenesis. The purpose of the present study was to determine whether markers of endothelial progenitor cells (EPCs) are present in choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD). METHODS. Surgically excised CNV (n = 9) membranes from patients with AMD were probed with immunohistochemical techniques using the following monoclonal antibodies: AC133 a putative marker of EPCs and hematopoietic stem cells (HSCs); the endothelial cells markers CD31, CD34, and von Willebrand factor (vWF); and cytokeratins and CD68, markers for retinal pigment epithelium (RPE) and macrophages, respectively. After secondary antibody amplification, reactions were visualized with fast red substrate. RESULTS. Six of nine specimens demonstrated cells positive for AC133 that were all found within predominantly cellular regions of the specimens. In the avascular fibrous stromal core of all specimens, the predominant cells were RPE cells and macrophages. The peripheral component of all CNV membranes was highly vascular and showed varying immunoreactivity for all endothelial markers. The greatest immunoreactivity for endothelial markers was observed with CD34 and vWF and least for CD31. CONCLUSIONS. These findings support animal studies that vasculogenesis, in addition to angiogenesis, may contribute to the neovascularization that occurs in AMD. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/146293
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSheridan, CMen_HK
dc.contributor.authorRice, Den_HK
dc.contributor.authorHiscott, PSen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorKent, DLen_HK
dc.date.accessioned2012-04-10T01:50:00Z-
dc.date.available2012-04-10T01:50:00Z-
dc.date.issued2006en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2006, v. 47 n. 4, p. 1642-1645en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146293-
dc.description.abstractPURPOSE. Recent evidence suggests that vasculogenesis as well as angiogenesis occurs throughout the body during neovascularization. The recruitment of circulating stem cells is a key feature of vasculogenesis. The purpose of the present study was to determine whether markers of endothelial progenitor cells (EPCs) are present in choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD). METHODS. Surgically excised CNV (n = 9) membranes from patients with AMD were probed with immunohistochemical techniques using the following monoclonal antibodies: AC133 a putative marker of EPCs and hematopoietic stem cells (HSCs); the endothelial cells markers CD31, CD34, and von Willebrand factor (vWF); and cytokeratins and CD68, markers for retinal pigment epithelium (RPE) and macrophages, respectively. After secondary antibody amplification, reactions were visualized with fast red substrate. RESULTS. Six of nine specimens demonstrated cells positive for AC133 that were all found within predominantly cellular regions of the specimens. In the avascular fibrous stromal core of all specimens, the predominant cells were RPE cells and macrophages. The peripheral component of all CNV membranes was highly vascular and showed varying immunoreactivity for all endothelial markers. The greatest immunoreactivity for endothelial markers was observed with CD34 and vWF and least for CD31. CONCLUSIONS. These findings support animal studies that vasculogenesis, in addition to angiogenesis, may contribute to the neovascularization that occurs in AMD. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAntibodies, Monoclonal - Metabolismen_US
dc.subject.meshAntigens, Cd - Metabolismen_US
dc.subject.meshBiological Markers - Metabolismen_US
dc.subject.meshChoroid - Blood Supplyen_US
dc.subject.meshChoroidal Neovascularization - Etiology - Pathologyen_US
dc.subject.meshEndothelium, Vascular - Metabolism - Pathologyen_US
dc.subject.meshGlycoproteins - Metabolismen_US
dc.subject.meshHematopoietic Stem Cells - Metabolism - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMacular Degeneration - Complicationsen_US
dc.subject.meshPeptides - Metabolismen_US
dc.titleThe presence of AC133-positive cells suggests a possible role of endothelial progenitor cells in the formation of choroidal neovascularizationen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1167/iovs.05-0779en_HK
dc.identifier.pmid16565404-
dc.identifier.scopuseid_2-s2.0-33645988867en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645988867&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1642en_HK
dc.identifier.epage1645en_HK
dc.identifier.isiWOS:000236560800051-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSheridan, CM=7004974390en_HK
dc.identifier.scopusauthoridRice, D=13005597200en_HK
dc.identifier.scopusauthoridHiscott, PS=7006368693en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridKent, DL=35512364500en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats