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Article: Distribution of sparc in PVR epiretinal membranes and production of sparc by cultured human retinal pigment epithelial cells

TitleDistribution of sparc in PVR epiretinal membranes and production of sparc by cultured human retinal pigment epithelial cells
Authors
Issue Date1997
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 1997, v. 38 n. 4, p. S671 How to Cite?
AbstractPurpose SPARC (secreted protein acid rich in cysteine) is believed to modulate cell adhesion to the extracellular matrix We investigated the hypothesis that SPARC is associated with migratory retinal epithelial cells in proliferative vitreoretinopathy (PVR) epiretinal membranes and that cultured human retinal pigment epithelial (HRPE) cells may synthesise SPARC Methods Light microscopic immunohistochemistry was used to examine epiretinal membranes for the presence of SPARC and migratory HRPE whilst combined with immunoblotting and immunoprecipitation, the possible production of SPARC by HRPE vitro was examined. Results SPARC immunostaining was observed associated with migratory HRPE (identified through the expression of a migration-related epitope expressed on RPE cytokeratin 18) in epiretinal membranes In cultures of HRPE the production of SPARC was identified as a band in cell lysates and conditioned media. Conclusions Our results suggest that HRPE are capable of SPARC synthesis The association between SPARC and migratory RPE cells in epiretinal membranes may indicate an important role for this matricellular protein in PVR development. Supported by the Guide Dogs for the Blind Association, North West Regional Health Authority and The Royal College of Surgeons of Edinburgh. None.
Persistent Identifierhttp://hdl.handle.net/10722/146291
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008

 

DC FieldValueLanguage
dc.contributor.authorMagee, RMen_HK
dc.contributor.authorSheridan, Cen_HK
dc.contributor.authorHagan, Sen_HK
dc.contributor.authorHiscott, PSen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorMcGalliard, Jen_HK
dc.date.accessioned2012-04-10T01:49:59Z-
dc.date.available2012-04-10T01:49:59Z-
dc.date.issued1997en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 1997, v. 38 n. 4, p. S671en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146291-
dc.description.abstractPurpose SPARC (secreted protein acid rich in cysteine) is believed to modulate cell adhesion to the extracellular matrix We investigated the hypothesis that SPARC is associated with migratory retinal epithelial cells in proliferative vitreoretinopathy (PVR) epiretinal membranes and that cultured human retinal pigment epithelial (HRPE) cells may synthesise SPARC Methods Light microscopic immunohistochemistry was used to examine epiretinal membranes for the presence of SPARC and migratory HRPE whilst combined with immunoblotting and immunoprecipitation, the possible production of SPARC by HRPE vitro was examined. Results SPARC immunostaining was observed associated with migratory HRPE (identified through the expression of a migration-related epitope expressed on RPE cytokeratin 18) in epiretinal membranes In cultures of HRPE the production of SPARC was identified as a band in cell lysates and conditioned media. Conclusions Our results suggest that HRPE are capable of SPARC synthesis The association between SPARC and migratory RPE cells in epiretinal membranes may indicate an important role for this matricellular protein in PVR development. Supported by the Guide Dogs for the Blind Association, North West Regional Health Authority and The Royal College of Surgeons of Edinburgh. None.en_HK
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.titleDistribution of sparc in PVR epiretinal membranes and production of sparc by cultured human retinal pigment epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-25944446110en_HK
dc.identifier.volume38en_HK
dc.identifier.issue4en_HK
dc.identifier.spageS671en_HK
dc.identifier.epageS671en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMagee, RM=14820645000en_HK
dc.identifier.scopusauthoridSheridan, C=7004974390en_HK
dc.identifier.scopusauthoridHagan, S=7003335246en_HK
dc.identifier.scopusauthoridHiscott, PS=7006368693en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridMcGalliard, J=7003380072en_HK

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