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Article: Effects of the matricellular protein SPARC on human retinal pigment epithelial cell behavior

TitleEffects of the matricellular protein SPARC on human retinal pigment epithelial cell behavior
Authors
Issue Date2003
PublisherMolecular Vision. The Journal's web site is located at http://www.molvis.org/molvis/
Citation
Molecular Vision, 2003, v. 9, p. 87-92 How to Cite?
AbstractPurpose: To determine the effects of the matricellular protein SPARC (Secreted Protein, Acidic and Rich in Cysteine) on human retinal pigment epithelial (HRPE) cell behavior in vitro. Methods: Proliferation and migration assays were performed on HRPE cells exposed to various concentrations of SPARC. Additionally, HRPE cells were seeded on top of collagen matrices (a 2D model of the retinal scarring disorder known as proliferative vitreoretinopathy or PVR) and were exposed to SPARC over a 7 day period. Changes in matrix contraction were recorded. Results: HRPE cell proliferation was significantly inhibited at 1 and 10 μg/ml SPARC (p<0.01). SPARC protein did not stimulate HRPE cell migration at any of the concentrations used. SPARC did not significantly affect fibronectin-induced HRPE cell migration at SPARC concentrations up to 10 μg/ml. HRPE cell-seeded collagen matrices demonstrated a significant inhibition of matrix contraction by 1 and 10 μg/ml SPARC (t-test; p<0.02 and 0.001, respectively) compared to controls. Conclusions: SPARC protein has anti-proliferative effects on HRPE cells in vitro. In addition, SPARC appears to have an inhibitory effect on HRPE-mediated contraction of 2D collagen matrices. These results are consistent with an important role for SPARC in modulating cell behavior in vitro and may indicate a role for SPARC in modifying HRPE cell activities during the development of PVR and other proliferative retinal diseases.
Persistent Identifierhttp://hdl.handle.net/10722/146270
ISSN
2015 Impact Factor: 2.11
2015 SCImago Journal Rankings: 1.037
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHagan, Sen_HK
dc.contributor.authorHiscott, Pen_HK
dc.contributor.authorSheridan, CMen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorGrierson, Ien_HK
dc.contributor.authorMcGalliard, Jen_HK
dc.date.accessioned2012-04-10T01:49:50Z-
dc.date.available2012-04-10T01:49:50Z-
dc.date.issued2003en_HK
dc.identifier.citationMolecular Vision, 2003, v. 9, p. 87-92en_HK
dc.identifier.issn1090-0535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146270-
dc.description.abstractPurpose: To determine the effects of the matricellular protein SPARC (Secreted Protein, Acidic and Rich in Cysteine) on human retinal pigment epithelial (HRPE) cell behavior in vitro. Methods: Proliferation and migration assays were performed on HRPE cells exposed to various concentrations of SPARC. Additionally, HRPE cells were seeded on top of collagen matrices (a 2D model of the retinal scarring disorder known as proliferative vitreoretinopathy or PVR) and were exposed to SPARC over a 7 day period. Changes in matrix contraction were recorded. Results: HRPE cell proliferation was significantly inhibited at 1 and 10 μg/ml SPARC (p<0.01). SPARC protein did not stimulate HRPE cell migration at any of the concentrations used. SPARC did not significantly affect fibronectin-induced HRPE cell migration at SPARC concentrations up to 10 μg/ml. HRPE cell-seeded collagen matrices demonstrated a significant inhibition of matrix contraction by 1 and 10 μg/ml SPARC (t-test; p<0.02 and 0.001, respectively) compared to controls. Conclusions: SPARC protein has anti-proliferative effects on HRPE cells in vitro. In addition, SPARC appears to have an inhibitory effect on HRPE-mediated contraction of 2D collagen matrices. These results are consistent with an important role for SPARC in modulating cell behavior in vitro and may indicate a role for SPARC in modifying HRPE cell activities during the development of PVR and other proliferative retinal diseases.en_HK
dc.languageengen_US
dc.publisherMolecular Vision. The Journal's web site is located at http://www.molvis.org/molvis/en_HK
dc.relation.ispartofMolecular Visionen_HK
dc.subject.meshAdulten_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Movement - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagen - Metabolismen_US
dc.subject.meshExtracellular Matrix - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibronectins - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshMaleen_US
dc.subject.meshOsteonectin - Pharmacologyen_US
dc.subject.meshPigment Epithelium Of Eye - Cytologyen_US
dc.titleEffects of the matricellular protein SPARC on human retinal pigment epithelial cell behavioren_HK
dc.typeArticleen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid12655283-
dc.identifier.scopuseid_2-s2.0-0037464186en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037464186&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.spage87en_HK
dc.identifier.epage92en_HK
dc.identifier.isiWOS:000181774400002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHagan, S=7003335246en_HK
dc.identifier.scopusauthoridHiscott, P=7006368693en_HK
dc.identifier.scopusauthoridSheridan, CM=7004974390en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridGrierson, I=7005212606en_HK
dc.identifier.scopusauthoridMcGalliard, J=7003380072en_HK

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