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Article: Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: Visual results and histopathological findings

TitleTrypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: Visual results and histopathological findings
Authors
Issue Date2003
PublisherBMJ Publishing Group. The Journal's web site is located at http://bjo.bmjjournals.com/
Citation
British Journal Of Ophthalmology, 2003, v. 87 n. 2, p. 216-219 How to Cite?
AbstractAims: To report on the use of trypan blue (TB) 0.06% for staining the internal limiting membrane (ILM) and epiretinal membrane (ERM) during vitrectomy and report on their histology. Method: 14 consecutive patients with idiopathic macular hole or macular pucker (seven patients each) were prospectively recruited for ILM or ERM peel respectively. After pars plana vitrectomy and induction of posterior vitreous detachment, 0.5 ml TB 0.06% in phosphate buffered saline (VisonBlue) was injected over the posterior pole in an air filled eye and left for 2 minutes. The stained tissue was peeled with intraocular forceps. Specimens were evaluated using histochemical and immunohistochemical methods. Results: The average follow up was 4.4 months. Internal limiting membranes and epiretinal membranes were stained satisfactorily in all cases and removed successfully. Eight patients (57%) had improvement of 2 or more Snellen lines. All seven macular holes closed. In the ERM cases, no residual membranes were observed clinically, at the latest follow up. No complicatians relating to the use of the dye were encountered intraoperatively or postoperatively. Of the 14 procedures, nine (four macular hole and five macular pucker) yielded sufficient tissue for histopathological evaluation. Histological and immunohistological assessment revealed that the morphology of these specimens was similar to that observed in macular hole ILM and macular pucker ERM removed without the aid of dye. Conclusion: TB staining facilitated the identification and delineation of ILM and ERM removal during the surgical management of macular holes and macular pucker. The visual outcome of this series and the specimens removed suggest they are no different from those without TB staining. Its use in posterior segment appears to be safe but further studies are required to investigate its long term safety.
Persistent Identifierhttp://hdl.handle.net/10722/146268
ISSN
2015 Impact Factor: 3.036
2015 SCImago Journal Rankings: 1.873
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Ken_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorHiscott, Pen_HK
dc.contributor.authorStanga, Pen_HK
dc.contributor.authorGroenewald, Cen_HK
dc.contributor.authorMcGalliard, Jen_HK
dc.date.accessioned2012-04-10T01:49:49Z-
dc.date.available2012-04-10T01:49:49Z-
dc.date.issued2003en_HK
dc.identifier.citationBritish Journal Of Ophthalmology, 2003, v. 87 n. 2, p. 216-219en_HK
dc.identifier.issn0007-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146268-
dc.description.abstractAims: To report on the use of trypan blue (TB) 0.06% for staining the internal limiting membrane (ILM) and epiretinal membrane (ERM) during vitrectomy and report on their histology. Method: 14 consecutive patients with idiopathic macular hole or macular pucker (seven patients each) were prospectively recruited for ILM or ERM peel respectively. After pars plana vitrectomy and induction of posterior vitreous detachment, 0.5 ml TB 0.06% in phosphate buffered saline (VisonBlue) was injected over the posterior pole in an air filled eye and left for 2 minutes. The stained tissue was peeled with intraocular forceps. Specimens were evaluated using histochemical and immunohistochemical methods. Results: The average follow up was 4.4 months. Internal limiting membranes and epiretinal membranes were stained satisfactorily in all cases and removed successfully. Eight patients (57%) had improvement of 2 or more Snellen lines. All seven macular holes closed. In the ERM cases, no residual membranes were observed clinically, at the latest follow up. No complicatians relating to the use of the dye were encountered intraoperatively or postoperatively. Of the 14 procedures, nine (four macular hole and five macular pucker) yielded sufficient tissue for histopathological evaluation. Histological and immunohistological assessment revealed that the morphology of these specimens was similar to that observed in macular hole ILM and macular pucker ERM removed without the aid of dye. Conclusion: TB staining facilitated the identification and delineation of ILM and ERM removal during the surgical management of macular holes and macular pucker. The visual outcome of this series and the specimens removed suggest they are no different from those without TB staining. Its use in posterior segment appears to be safe but further studies are required to investigate its long term safety.en_HK
dc.languageengen_US
dc.publisherBMJ Publishing Group. The Journal's web site is located at http://bjo.bmjjournals.com/en_HK
dc.relation.ispartofBritish Journal of Ophthalmologyen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistry - Methodsen_US
dc.subject.meshMacula Lutea - Pathology - Surgeryen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshRetinal Perforations - Pathology - Surgeryen_US
dc.subject.meshTreatment Outcomeen_US
dc.subject.meshTrypan Blue - Diagnostic Useen_US
dc.subject.meshVitrectomy - Methodsen_US
dc.titleTrypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: Visual results and histopathological findingsen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1136/bjo.87.2.216en_HK
dc.identifier.pmid12543755-
dc.identifier.pmcidPMC1771493-
dc.identifier.scopuseid_2-s2.0-0037310761en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037310761&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue2en_HK
dc.identifier.spage216en_HK
dc.identifier.epage219en_HK
dc.identifier.isiWOS:000180695300021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, K=7404990010en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridHiscott, P=7006368693en_HK
dc.identifier.scopusauthoridStanga, P=6602197864en_HK
dc.identifier.scopusauthoridGroenewald, C=6601917086en_HK
dc.identifier.scopusauthoridMcGalliard, J=7003380072en_HK

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