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Article: The role of matricellular proteins thrombospondin-1 and osteonectin during RPE cell migration in proliferative vitreoretinopathy

TitleThe role of matricellular proteins thrombospondin-1 and osteonectin during RPE cell migration in proliferative vitreoretinopathy
Authors
KeywordsCell migration
Epiretinal membranes
Osteonectin
RPE
Thrombospondin
Issue Date2002
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/02713683.asp
Citation
Current Eye Research, 2002, v. 25 n. 5, p. 279-285 How to Cite?
AbstractPurpose: To investigate the hypothesis that the Matricellular proteins thrombospondin 1 (TSP1), tenascin (TN) and Secreted Protein Acidic and Rich in Cysteine (SPARC) modulate the migration of RPE cells in the epiretinal membranes of proliferative vitreoretinopathy. Methods: Ten PVR epiretinal membranes were studied by immunohistochemical methods in which aggregates of RPE cells were identified by their expression of a broad range of cytokeratins. RPE subsets containing migratory RPE cells were detected by immunoreactivity for the monoclonal antibody RGE53 (which detects an epitope on cytokeratin-18 on motile RPE cells). Co-localisation of the RPE subsets with the glycoproteins TSP-1, SPARC and TN was evaluated. Results: Nineteen migratory RPE (RGE53 positive) subsets and 13 RPE (RGE53 negative) subsets were identified. All of the RGE53+ subsets colocalised with TSP1 and SPARC and 17 with TN. Ten of the RGE53- aggregates stained for TN, 6 for SPARC and 5 for TSP1. The association between the presence of RGE53+ cells in the RPE cell aggregates and TSP1 immunoreactivity in the aggregates was significant (p < 0.001), and there was a comparable significant association between RGE53+ cells and SPARC (p < 0.001). No such association was detected for RGE53+ cells and TN (p > 0.2). Conclusions: The findings support the concept that the migration of retinal pigment cells in epiretinal membranes is modulated by TSP1 and SPARC and thus that these two proteins ultimately may represent therapeutic targets in the management of the membranes.
Persistent Identifierhttp://hdl.handle.net/10722/146267
ISSN
2015 Impact Factor: 2.025
2015 SCImago Journal Rankings: 0.975
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSheridan, CMen_HK
dc.contributor.authorMagee, RMen_HK
dc.contributor.authorHiscott, PSen_HK
dc.contributor.authorHagan, Sen_HK
dc.contributor.authorWong, DHen_HK
dc.contributor.authorMcGalliard, JNen_HK
dc.contributor.authorGrierson, Ien_HK
dc.date.accessioned2012-04-10T01:49:49Z-
dc.date.available2012-04-10T01:49:49Z-
dc.date.issued2002en_HK
dc.identifier.citationCurrent Eye Research, 2002, v. 25 n. 5, p. 279-285en_HK
dc.identifier.issn0271-3683en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146267-
dc.description.abstractPurpose: To investigate the hypothesis that the Matricellular proteins thrombospondin 1 (TSP1), tenascin (TN) and Secreted Protein Acidic and Rich in Cysteine (SPARC) modulate the migration of RPE cells in the epiretinal membranes of proliferative vitreoretinopathy. Methods: Ten PVR epiretinal membranes were studied by immunohistochemical methods in which aggregates of RPE cells were identified by their expression of a broad range of cytokeratins. RPE subsets containing migratory RPE cells were detected by immunoreactivity for the monoclonal antibody RGE53 (which detects an epitope on cytokeratin-18 on motile RPE cells). Co-localisation of the RPE subsets with the glycoproteins TSP-1, SPARC and TN was evaluated. Results: Nineteen migratory RPE (RGE53 positive) subsets and 13 RPE (RGE53 negative) subsets were identified. All of the RGE53+ subsets colocalised with TSP1 and SPARC and 17 with TN. Ten of the RGE53- aggregates stained for TN, 6 for SPARC and 5 for TSP1. The association between the presence of RGE53+ cells in the RPE cell aggregates and TSP1 immunoreactivity in the aggregates was significant (p < 0.001), and there was a comparable significant association between RGE53+ cells and SPARC (p < 0.001). No such association was detected for RGE53+ cells and TN (p > 0.2). Conclusions: The findings support the concept that the migration of retinal pigment cells in epiretinal membranes is modulated by TSP1 and SPARC and thus that these two proteins ultimately may represent therapeutic targets in the management of the membranes.en_HK
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/02713683.aspen_HK
dc.relation.ispartofCurrent Eye Researchen_HK
dc.subjectCell migrationen_HK
dc.subjectEpiretinal membranesen_HK
dc.subjectOsteonectinen_HK
dc.subjectRPEen_HK
dc.subjectThrombospondinen_HK
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Movement - Physiologyen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshEpiretinal Membrane - Metabolism - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMembrane Glycoproteins - Physiologyen_US
dc.subject.meshOsteonectin - Physiologyen_US
dc.subject.meshPigment Epithelium Of Eye - Pathology - Physiologyen_US
dc.subject.meshTenascin - Physiologyen_US
dc.subject.meshThrombospondin 1 - Physiologyen_US
dc.subject.meshVitreoretinopathy, Proliferative - Metabolism - Pathologyen_US
dc.titleThe role of matricellular proteins thrombospondin-1 and osteonectin during RPE cell migration in proliferative vitreoretinopathyen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, DH: shdwong@hku.hken_HK
dc.identifier.authorityWong, DH=rp00516en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1076/ceyr.25.5.279.13492en_HK
dc.identifier.pmid12658547-
dc.identifier.scopuseid_2-s2.0-0036875832en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036875832&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue5en_HK
dc.identifier.spage279en_HK
dc.identifier.epage285en_HK
dc.identifier.isiWOS:000182203800004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSheridan, CM=7004974390en_HK
dc.identifier.scopusauthoridMagee, RM=14820645000en_HK
dc.identifier.scopusauthoridHiscott, PS=7006368693en_HK
dc.identifier.scopusauthoridHagan, S=7003335246en_HK
dc.identifier.scopusauthoridWong, DH=7401536078en_HK
dc.identifier.scopusauthoridMcGalliard, JN=7003380072en_HK
dc.identifier.scopusauthoridGrierson, I=7005212606en_HK

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