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postgraduate thesis: A pilot study on investigation of Chinese medicinal classification andits relationship with hormones in menopausal women

TitleA pilot study on investigation of Chinese medicinal classification andits relationship with hormones in menopausal women
Authors
Advisors
Advisor(s):Tong, YSze, CW
Issue Date2011
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
AbstractObjective: To develop a validated questionnaire for the assessment of menopausal syndrome in Chinese Medicine and through the analysis of the basic menopausal syndrome classification in Chinese Medicine to explore the use of objective hormone markers as one of the evidence for the Chinese Medicinal classification and guidance for application of herbal medicines by Chinese Medicine Practitioners. Methods: The major symptoms related to typical syndromes of menopause were extracted from Chinese Medicine textbook, international diagnostic guide of Chinese Medicine, classic and contemporary literature of Chinese Medicine and subjective symptoms were eliminated to facilitate the administrate of a self-reported outcome questionnaire. The symptoms related to menopause in Chinese Medicine were identified and redefined to improve the content validity. A total of 353 menopausal women were included in the study. Participants were assessed through face-to-face interview by registered Hong Kong Chinese Medicine Practitioner to complete the questionnaire and serum samples for hormones measurement were collected after an overnight fast in the morning of the assessment day. Serum Estradiol (E2), Testosterone (T), Progesterone (P), Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and Sex-Hormone Binding Globulin (SHBG) were measured by electrochemiluminescence immunoassays (ECLIA). Free estradiol (fE2), non-Sex-Hormone Binding Globulin bound Estradiol (non-SHBG-E2), free Testosterone (fT), and non-Sex-Hormone Binding Globulin bound Testosterone (non-SHBG-T) were calculated to assess hormone availability and activity. E2/T ratio was also calculated to investigate the balance of E2 and T in the body. Results: Principal Component Analysis (PCA) was conducted. Symptoms related to Deficiency of Yin and symptoms related to Deficiency of Yang were extracted and the relationship between the syndromes and E2, T, P, LH, FSH, SHBG, fT, non-SHBG-T, fE2, non-SHBG-E2 and E2/T ratio were investigated. For symptoms related to Deficiency of Yin, E2 (p<0.000, p<0.000), P (p=0.004, p<0.000), fE2 (p<0.000, p<0.000), non-SHBG-E2 (p<0.000, p<0.000), E2/T (p<0.000, p<0.000),SHBG (p=0.039, p=0.004) showed significant decrease while LH (p=0.013, p<0.000), FSH (p=0.041, p<0.000) showed increase by comparing hormones of mild or little with moderate and severe presentation of symptoms. For symptoms related to Deficiency of Yang, SHBG (p=0.042, p=0.002, p<0.000) showed an increase, fT (p=0.044, p=0.049, p=0.003) and non-SHBG-T (p=0.044, p=0.049, p=0.003) showed a decrease which was significant for other three groups while comparing to the no or little group. Conclusion: Menopausal Syndrome Questionnaire was developed and validated in the study. Major syndromes and symptoms involved in the diagnosis of menopausal syndrome in Chinese Medicine were identified and good test-retest reliability was also demonstrated. From the result, we hypothesize that the presentation of symptoms related to Deficiency of Yin may be closely correlated with the menopausal transition in Western Medicine and the presentation of symptoms related to Deficiency of Yang may be related to deficiency of bio-active T in menopausal women. The study may provide further information on the relationship between reproductive hormones and Chinese Medicinal Classification and provide evidences and guides for effective treatment of menopausal syndrome in Chinese Medicine.
DegreeMaster of Philosophy
SubjectMenopause.
Dept/ProgramChinese Medicine

 

DC FieldValueLanguage
dc.contributor.advisorTong, Y-
dc.contributor.advisorSze, CW-
dc.contributor.authorLam, Lam.-
dc.contributor.author林琳.-
dc.date.issued2011-
dc.description.abstractObjective: To develop a validated questionnaire for the assessment of menopausal syndrome in Chinese Medicine and through the analysis of the basic menopausal syndrome classification in Chinese Medicine to explore the use of objective hormone markers as one of the evidence for the Chinese Medicinal classification and guidance for application of herbal medicines by Chinese Medicine Practitioners. Methods: The major symptoms related to typical syndromes of menopause were extracted from Chinese Medicine textbook, international diagnostic guide of Chinese Medicine, classic and contemporary literature of Chinese Medicine and subjective symptoms were eliminated to facilitate the administrate of a self-reported outcome questionnaire. The symptoms related to menopause in Chinese Medicine were identified and redefined to improve the content validity. A total of 353 menopausal women were included in the study. Participants were assessed through face-to-face interview by registered Hong Kong Chinese Medicine Practitioner to complete the questionnaire and serum samples for hormones measurement were collected after an overnight fast in the morning of the assessment day. Serum Estradiol (E2), Testosterone (T), Progesterone (P), Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and Sex-Hormone Binding Globulin (SHBG) were measured by electrochemiluminescence immunoassays (ECLIA). Free estradiol (fE2), non-Sex-Hormone Binding Globulin bound Estradiol (non-SHBG-E2), free Testosterone (fT), and non-Sex-Hormone Binding Globulin bound Testosterone (non-SHBG-T) were calculated to assess hormone availability and activity. E2/T ratio was also calculated to investigate the balance of E2 and T in the body. Results: Principal Component Analysis (PCA) was conducted. Symptoms related to Deficiency of Yin and symptoms related to Deficiency of Yang were extracted and the relationship between the syndromes and E2, T, P, LH, FSH, SHBG, fT, non-SHBG-T, fE2, non-SHBG-E2 and E2/T ratio were investigated. For symptoms related to Deficiency of Yin, E2 (p<0.000, p<0.000), P (p=0.004, p<0.000), fE2 (p<0.000, p<0.000), non-SHBG-E2 (p<0.000, p<0.000), E2/T (p<0.000, p<0.000),SHBG (p=0.039, p=0.004) showed significant decrease while LH (p=0.013, p<0.000), FSH (p=0.041, p<0.000) showed increase by comparing hormones of mild or little with moderate and severe presentation of symptoms. For symptoms related to Deficiency of Yang, SHBG (p=0.042, p=0.002, p<0.000) showed an increase, fT (p=0.044, p=0.049, p=0.003) and non-SHBG-T (p=0.044, p=0.049, p=0.003) showed a decrease which was significant for other three groups while comparing to the no or little group. Conclusion: Menopausal Syndrome Questionnaire was developed and validated in the study. Major syndromes and symptoms involved in the diagnosis of menopausal syndrome in Chinese Medicine were identified and good test-retest reliability was also demonstrated. From the result, we hypothesize that the presentation of symptoms related to Deficiency of Yin may be closely correlated with the menopausal transition in Western Medicine and the presentation of symptoms related to Deficiency of Yang may be related to deficiency of bio-active T in menopausal women. The study may provide further information on the relationship between reproductive hormones and Chinese Medicinal Classification and provide evidences and guides for effective treatment of menopausal syndrome in Chinese Medicine.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.source.urihttp://hub.hku.hk/bib/B47251177-
dc.subject.lcshMenopause.-
dc.titleA pilot study on investigation of Chinese medicinal classification andits relationship with hormones in menopausal women-
dc.typePG_Thesis-
dc.identifier.hkulb4725117-
dc.description.thesisnameMaster of Philosophy-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineChinese Medicine-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.5353/th_b4725117-
dc.date.hkucongregation2012-

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