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Article: Complete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus

TitleComplete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virus
Authors
KeywordsFull genome sequencing
Phylogenetic analysis
PRRSVRT-PCR
Issue Date2012
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569
Citation
Virus Genes, 2012, v. 44 n. 1, p. 51-54 How to Cite?
AbstractPorcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84% identity/89% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena. © Springer Science+Business Media, LLC 2011.
Persistent Identifierhttp://hdl.handle.net/10722/146037
ISSN
2015 Impact Factor: 1.285
2015 SCImago Journal Rankings: 0.767
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorVan Doorsselaere, Jen_HK
dc.contributor.authorBrar, MSen_HK
dc.contributor.authorShi, Men_HK
dc.contributor.authorKarniychuk, Uen_HK
dc.contributor.authorLeung, FCCen_HK
dc.contributor.authorNauwynck, HJen_HK
dc.date.accessioned2012-03-27T09:07:32Z-
dc.date.available2012-03-27T09:07:32Z-
dc.date.issued2012en_HK
dc.identifier.citationVirus Genes, 2012, v. 44 n. 1, p. 51-54en_HK
dc.identifier.issn0920-8569en_HK
dc.identifier.urihttp://hdl.handle.net/10722/146037-
dc.description.abstractPorcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. PRRSV strains are divided into European (Type 1) and North-American (Type 2) genotypes. Within the European PRRSV genotype, three subtypes have been delineated. Full genome sequences for North American and European subtype 1 strains have been described. Here, the first complete genomic characterization of a European subtype 3 strain (Lena) is described. Amplification of Orf1a and Orf1b fragments was achieved using a set of degenerate oligonucleotides. Using RT-PCR with Lena-specific primers, the full length sequence (15001 nt) was obtained. Alignment of Lena with European subtype 1 reference strain Lelystad showed variation over the entire length (84% identity/89% similarity at amino acid level) with the most variation in Orf1a (Nsp2/NSP2) with a deletion of 29 amino acids. Phylogenetic relationships using different Orfs supported Lena's genetic distinction from European subtype 1 strains. The availability of the European subtype 3 PRRSV full genome may be important for the understanding of PRRSV evolution and the more pronounced pathogenic nature of Lena. © Springer Science+Business Media, LLC 2011.en_HK
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569en_HK
dc.relation.ispartofVirus Genesen_HK
dc.subjectFull genome sequencingen_HK
dc.subjectPhylogenetic analysisen_HK
dc.subjectPRRSVRT-PCRen_HK
dc.titleComplete genome characterization of a East European Type 1 subtype 3 porcine reproductive and respiratory syndrome virusen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11262-011-0665-xen_HK
dc.identifier.pmid21948018-
dc.identifier.pmcidPMC21948018-
dc.identifier.scopuseid_2-s2.0-84863717992en_HK
dc.identifier.hkuros199086en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863717992&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue1en_HK
dc.identifier.spage51en_HK
dc.identifier.epage54en_HK
dc.identifier.isiWOS:000299510900008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridVan Doorsselaere, J=6603341392en_HK
dc.identifier.scopusauthoridBrar, MS=44161059200en_HK
dc.identifier.scopusauthoridShi, M=25951822000en_HK
dc.identifier.scopusauthoridKarniychuk, U=26537572500en_HK
dc.identifier.scopusauthoridLeung, FCC=7103078633en_HK
dc.identifier.scopusauthoridNauwynck, HJ=7007141390en_HK
dc.identifier.citeulike9847865-

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