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Article: Bioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surface

TitleBioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surface
Authors
KeywordsAdhesion
Biofilm
Candida albicans
Poly(methyl methacrylate)
Surface properties
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/
Citation
Journal Of Biomedical Materials Research - Part B Applied Biomaterials, 2010, v. 94 n. 1, p. 149-156 How to Cite?
AbstractThe aim of this study was to evaluate the bioactivity and architecture of Candida albicans biofilms developed on the surface of poly(methyl methacrylate) (PMMA) resin. To do this, surface roughness (SR) and surface free energy of PMMA specimens were measured. Next, the biofilms of two different C. albicans strains (ATCC 90028 and SC5314) were allowed to develop on the PMMA surface and evaluated at 24, 48, and 72 h after adhesion. The bioactivity of the biofilms was measured by the XTT reduction assay. Biofilm topography was evaluated by scanning electron microscopy. Confocal microscopy was used to evaluate the architectural properties of bio-volume, average thickness, biofilm roughness, surface area/volume ratio and the proportion of live/dead cells in the different biofilm development stages. SR and SFE had no influence on biofilm development. Each strain exhibited a different biofilm activity (P < 0.001). Confocal images showed different architectures for the different biofilm development stages. We conclude that the main differences detected in biofilm bioactivity and architecture were related to the characteristics of each C. albicans strain and to biofilm development time. © 2010 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/145807
ISSN
2015 Impact Factor: 2.881
2015 SCImago Journal Rankings: 0.784
ISI Accession Number ID
Funding AgencyGrant Number
CAPESPDEE/BEX 4621/06-9
Funding Information:

Contract grant sponsor: CAPES; contract grant number: PDEE/BEX 4621/06-9

References

 

DC FieldValueLanguage
dc.contributor.authorDa Silva, WJen_HK
dc.contributor.authorSeneviratne, Jen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorDel Bel Cury, AAen_HK
dc.date.accessioned2012-03-23T02:17:04Z-
dc.date.available2012-03-23T02:17:04Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Biomedical Materials Research - Part B Applied Biomaterials, 2010, v. 94 n. 1, p. 149-156en_HK
dc.identifier.issn1552-4973en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145807-
dc.description.abstractThe aim of this study was to evaluate the bioactivity and architecture of Candida albicans biofilms developed on the surface of poly(methyl methacrylate) (PMMA) resin. To do this, surface roughness (SR) and surface free energy of PMMA specimens were measured. Next, the biofilms of two different C. albicans strains (ATCC 90028 and SC5314) were allowed to develop on the PMMA surface and evaluated at 24, 48, and 72 h after adhesion. The bioactivity of the biofilms was measured by the XTT reduction assay. Biofilm topography was evaluated by scanning electron microscopy. Confocal microscopy was used to evaluate the architectural properties of bio-volume, average thickness, biofilm roughness, surface area/volume ratio and the proportion of live/dead cells in the different biofilm development stages. SR and SFE had no influence on biofilm development. Each strain exhibited a different biofilm activity (P < 0.001). Confocal images showed different architectures for the different biofilm development stages. We conclude that the main differences detected in biofilm bioactivity and architecture were related to the characteristics of each C. albicans strain and to biofilm development time. © 2010 Wiley Periodicals, Inc.en_HK
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/en_HK
dc.relation.ispartofJournal of Biomedical Materials Research - Part B Applied Biomaterialsen_HK
dc.rightsJournal of Biomedical Materials Research Part B: Applied Biomaterials. Copyright © John Wiley & Sons, Inc.-
dc.subjectAdhesionen_HK
dc.subjectBiofilmen_HK
dc.subjectCandida albicansen_HK
dc.subjectPoly(methyl methacrylate)en_HK
dc.subjectSurface propertiesen_HK
dc.subject.meshBiofilms - growth and development-
dc.subject.meshCandida albicans - growth and development - ultrastructure-
dc.subject.meshCell Adhesion-
dc.subject.meshMicroscopy, Confocal-
dc.subject.meshPolymethyl Methacrylate - chemistry-
dc.titleBioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surfaceen_HK
dc.typeArticleen_HK
dc.identifier.emailSeneviratne, J:jaya@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySeneviratne, J=rp01372en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.b.31635en_HK
dc.identifier.pmid20524189-
dc.identifier.scopuseid_2-s2.0-77954141169en_HK
dc.identifier.hkuros170946-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954141169&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue1en_HK
dc.identifier.spage149en_HK
dc.identifier.epage156en_HK
dc.identifier.isiWOS:000278697400017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDa Silva, WJ=23972574300en_HK
dc.identifier.scopusauthoridSeneviratne, J=6701897753en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridDel Bel Cury, AA=35329490200en_HK

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