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Conference Paper: Novel and conventional assays determining streptococcus mutans abundance in saliva

TitleNovel and conventional assays determining streptococcus mutans abundance in saliva
Authors
Issue Date2010
PublisherInternational Association for Dental Research. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 Spec Iss C How to Cite?
AbstractOBJECTIVES: This study aimed to comparatively evaluate the validity of a novel immunoassay and a conventional assay for detecting salivary Streptococcus mutans in a pediatric cohort. METHODS: With an ethical approval from the University of Hong Kong Institutional Review Board (IRB #UW08-400), 190 preschool children aged 3-4 years were recruited from three kindergartens representing low, moderate, and high socio-economic strata in Hong Kong. The abundance of Streptococcus mutans in saliva samples was analyzed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay (SALIVA-CHECK MUTANS) based on monoclonal antibody technology, and a real-time PCR assay taken as a gold standard. RESULTS: The mean numbers of Streptococcus mutans [log10(CFU/ml saliva)] confirmed by real-time PCR were 4.75+/-1.56, 4.95+/-1.42, and 3.56+/-1.05 for low, moderate, and high socio-economic groups, respectively. Children from families of high socio-economic status harbored significantly lower level of Streptococcus mutans, compared with the rest of participants (p<0.001). The immunoassay accurately categorized salivary samples into two groups with high (≥5X105 CFU/ml) and low (<5X105 CFU/ml) levels of Streptococcus mutans. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with a moderate (≥104 CFU/ml) level of Streptococcus mutans. However, its sensitivity/specificity in selecting children with high (≥105 CFU/ml) and very high (>106 CFU/ml) levels of Streptococcus mutans were relatively low (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSIONS: The monoclonal antibody-based immunoassay, which accurately and rapidly determines Streptococcus mutans abundance in saliva, could be useful for chairside assessment of patients' caries risk.
DescriptionResearch Poster III: abstract no. 0083
Persistent Identifierhttp://hdl.handle.net/10722/145784
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorGao, X-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorLo, ECM-
dc.contributor.authorChu, CH-
dc.contributor.authorSamaranayake, LP-
dc.date.accessioned2012-03-06T08:03:09Z-
dc.date.available2012-03-06T08:03:09Z-
dc.date.issued2010-
dc.identifier.citationThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 Spec Iss C-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/145784-
dc.descriptionResearch Poster III: abstract no. 0083-
dc.description.abstractOBJECTIVES: This study aimed to comparatively evaluate the validity of a novel immunoassay and a conventional assay for detecting salivary Streptococcus mutans in a pediatric cohort. METHODS: With an ethical approval from the University of Hong Kong Institutional Review Board (IRB #UW08-400), 190 preschool children aged 3-4 years were recruited from three kindergartens representing low, moderate, and high socio-economic strata in Hong Kong. The abundance of Streptococcus mutans in saliva samples was analyzed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay (SALIVA-CHECK MUTANS) based on monoclonal antibody technology, and a real-time PCR assay taken as a gold standard. RESULTS: The mean numbers of Streptococcus mutans [log10(CFU/ml saliva)] confirmed by real-time PCR were 4.75+/-1.56, 4.95+/-1.42, and 3.56+/-1.05 for low, moderate, and high socio-economic groups, respectively. Children from families of high socio-economic status harbored significantly lower level of Streptococcus mutans, compared with the rest of participants (p<0.001). The immunoassay accurately categorized salivary samples into two groups with high (≥5X105 CFU/ml) and low (<5X105 CFU/ml) levels of Streptococcus mutans. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with a moderate (≥104 CFU/ml) level of Streptococcus mutans. However, its sensitivity/specificity in selecting children with high (≥105 CFU/ml) and very high (>106 CFU/ml) levels of Streptococcus mutans were relatively low (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSIONS: The monoclonal antibody-based immunoassay, which accurately and rapidly determines Streptococcus mutans abundance in saliva, could be useful for chairside assessment of patients' caries risk.-
dc.languageeng-
dc.publisherInternational Association for Dental Research. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Research-
dc.titleNovel and conventional assays determining streptococcus mutans abundance in salivaen_US
dc.typeConference_Paperen_US
dc.identifier.emailGao, X: gaoxl@hku.hk-
dc.identifier.emailSeneviratne, CJ: jaya@hku.hk-
dc.identifier.emailLo, ECM: edward-lo@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.emailSamaranayake, LP: lakshman@hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros181784-
dc.identifier.volume89-
dc.identifier.issueSpec Iss C-
dc.publisher.placeUnited States-
dc.description.otherThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 Spec Iss C-

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