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Conference Paper: Mineral and organic content of caries after silver diamine-fluoride application

TitleMineral and organic content of caries after silver diamine-fluoride application
Authors
Issue Date2010
Citation
The 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. How to Cite?
AbstractOBJECTIVE: To describe the effects of silver diamine fluoride (SDF) on mineral and organic content of dentine caries generated by Streptococcus mutans. METHOD: Human dentine blocks were demineralized with acetic acid (IRB-UW08-052). After demineralization, six blocks received 38% SDF solution (SDF group) whereas the other 6 blocks received distilled water (Control group). The blocks were placed in 24-well plates, inoculated with Streptococcus mutans, and incubated at 37℃ anaerobically for 7 days to form biofilm on the dentine caries lesions. Knoop microhardness testing was used to measure the microhardness of the lesions after biofilm challenge. Scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS) and Fourier transform infrared spectroscopy were used to study the calcium and phosphate content, and the organic (collagen I content using log [amide I: phosphate]) of the lesions, respectively. RESULTS: The Knoop hardness values of the outer 125 µm of the dentine caries lesions in SDF group was higher than the control group (0.34±0.08 vs. 0.14±0.02; p<0.01). SEM-EDS results showed calcium and phosphate weight-percent were higher in SDF group than the control group in outer 25 µm (Calcium: 20.3±8.0% vs. 6.0±4.1%, p<0.01; Phosphate: 9.3±2.8% vs 4.2±3.1%, p<0.05). The log [amide I: phosphate] of the lesions in SDF group was lower than the control group (0.65±0.26 vs. 1.10±0.28; p<0.05). CONCLUSION: The study results show that SDF can increase the microhardness and reduce the minerals loss and collagen I breakdown of dentine caries lesion generated by Streptococcus mutans.
DescriptionResearch Poster III: abstract no. 0078
Persistent Identifierhttp://hdl.handle.net/10722/145781

 

DC FieldValueLanguage
dc.contributor.authorChu, CH-
dc.contributor.authorMei, L-
dc.contributor.authorSeneviratne, CJ-
dc.contributor.authorLo, ECM-
dc.date.accessioned2012-03-06T03:36:20Z-
dc.date.available2012-03-06T03:36:20Z-
dc.date.issued2010-
dc.identifier.citationThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010.-
dc.identifier.urihttp://hdl.handle.net/10722/145781-
dc.descriptionResearch Poster III: abstract no. 0078-
dc.description.abstractOBJECTIVE: To describe the effects of silver diamine fluoride (SDF) on mineral and organic content of dentine caries generated by Streptococcus mutans. METHOD: Human dentine blocks were demineralized with acetic acid (IRB-UW08-052). After demineralization, six blocks received 38% SDF solution (SDF group) whereas the other 6 blocks received distilled water (Control group). The blocks were placed in 24-well plates, inoculated with Streptococcus mutans, and incubated at 37℃ anaerobically for 7 days to form biofilm on the dentine caries lesions. Knoop microhardness testing was used to measure the microhardness of the lesions after biofilm challenge. Scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS) and Fourier transform infrared spectroscopy were used to study the calcium and phosphate content, and the organic (collagen I content using log [amide I: phosphate]) of the lesions, respectively. RESULTS: The Knoop hardness values of the outer 125 µm of the dentine caries lesions in SDF group was higher than the control group (0.34±0.08 vs. 0.14±0.02; p<0.01). SEM-EDS results showed calcium and phosphate weight-percent were higher in SDF group than the control group in outer 25 µm (Calcium: 20.3±8.0% vs. 6.0±4.1%, p<0.01; Phosphate: 9.3±2.8% vs 4.2±3.1%, p<0.05). The log [amide I: phosphate] of the lesions in SDF group was lower than the control group (0.65±0.26 vs. 1.10±0.28; p<0.05). CONCLUSION: The study results show that SDF can increase the microhardness and reduce the minerals loss and collagen I breakdown of dentine caries lesion generated by Streptococcus mutans.-
dc.languageeng-
dc.relation.ispartofIADR-SEA Division Annual Scientific Meeting-
dc.titleMineral and organic content of caries after silver diamine-fluoride applicationen_US
dc.typeConference_Paperen_US
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.emailMei, L: leimei@hkusua.hku.hk-
dc.identifier.emailSeneviratne, CJ: jaya@hku.hk-
dc.identifier.emailLo, ECM: edward-lo@hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros181780-
dc.description.otherThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010.-

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