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Article: Baicalin promotes neuronal differentiation of neural stem/progenitor cells through modulating p-stat3 and bHLH family protein expression

TitleBaicalin promotes neuronal differentiation of neural stem/progenitor cells through modulating p-stat3 and bHLH family protein expression
Authors
KeywordsBaicalin
Differentiation
Neural progenitor cells
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2012, v. 1429, p. 36-42 How to Cite?
AbstractSignal transducer and activator of transcription 3 (stat3) and basic helix-loop-helix (bHLH) gene family are important cellular signal molecules for the regulation of cell fate decision and neuronal differentiation of neural stem/progenitor cells (NPCs). In the present study, we investigated the effects of baicalin, a flavonoid compound isolated from Scutellaria baicalensis G, on regulating phosphorylation of stat3 and expression of bHLH family proteins and promoting neuronal differentiation of NPCs. Embryonic NPCs from the cortex of E15-16 rats were treated with baicalin (2, 20 μM) for 2 h and 7 days. Neuronal and glial differentiations were identified with mature neuronal marker microtubule associated protein (MAP-2) and glial marker Glial fibrillary acidic protein (GFAP) immunostaining fluorescent microscopy respectively. Phosphorylation of stat3 (p-stat3) and expressions of bHLH family genes including Mash1, Hes1 and NeuroD1 were detected with immunofluorescent microscopy and Western blot analysis. The results revealed that baicalin treatment increased the percentages of MAP-2 positive staining cells and decreased GFAP staining cells. Meanwhile, baicalin treatment down-regulated the expression of p-stat3 and Hes1, but up-regulated the expressions of NeuroD1 and Mash1. Those results indicate that baicalin can promote the neural differentiation but inhibit glial formation and its neurogenesis-promoting effects are associated with the modulations of stat3 and bHLH genes in neural stem/progenitor cells. © 2011 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/145627
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200811 159111
RGC GRF777610M
National Science Foundation of China30472177
06YFSZSF0160
30873395/C190701
Funding Information:

This work was supported by Seeding Funding Programme for Basic Research in the University of Hong Kong (No. 200811 159111), RGC GRF grant (No. 777610M) and the National Science Foundation of China (No. 30472177; No. 06YFSZSF0160; No. 30873395/C190701).

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_HK
dc.contributor.authorZhuang, Pen_HK
dc.contributor.authorShen, Ben_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorShen, Jen_HK
dc.date.accessioned2012-02-28T01:59:10Z-
dc.date.available2012-02-28T01:59:10Z-
dc.date.issued2012en_HK
dc.identifier.citationBrain Research, 2012, v. 1429, p. 36-42en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145627-
dc.description.abstractSignal transducer and activator of transcription 3 (stat3) and basic helix-loop-helix (bHLH) gene family are important cellular signal molecules for the regulation of cell fate decision and neuronal differentiation of neural stem/progenitor cells (NPCs). In the present study, we investigated the effects of baicalin, a flavonoid compound isolated from Scutellaria baicalensis G, on regulating phosphorylation of stat3 and expression of bHLH family proteins and promoting neuronal differentiation of NPCs. Embryonic NPCs from the cortex of E15-16 rats were treated with baicalin (2, 20 μM) for 2 h and 7 days. Neuronal and glial differentiations were identified with mature neuronal marker microtubule associated protein (MAP-2) and glial marker Glial fibrillary acidic protein (GFAP) immunostaining fluorescent microscopy respectively. Phosphorylation of stat3 (p-stat3) and expressions of bHLH family genes including Mash1, Hes1 and NeuroD1 were detected with immunofluorescent microscopy and Western blot analysis. The results revealed that baicalin treatment increased the percentages of MAP-2 positive staining cells and decreased GFAP staining cells. Meanwhile, baicalin treatment down-regulated the expression of p-stat3 and Hes1, but up-regulated the expressions of NeuroD1 and Mash1. Those results indicate that baicalin can promote the neural differentiation but inhibit glial formation and its neurogenesis-promoting effects are associated with the modulations of stat3 and bHLH genes in neural stem/progenitor cells. © 2011 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.subjectBaicalinen_HK
dc.subjectDifferentiationen_HK
dc.subjectNeural progenitor cellsen_HK
dc.titleBaicalin promotes neuronal differentiation of neural stem/progenitor cells through modulating p-stat3 and bHLH family protein expressionen_HK
dc.typeArticleen_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainres.2011.10.030en_HK
dc.identifier.pmid22088824-
dc.identifier.scopuseid_2-s2.0-82255175377en_HK
dc.identifier.hkuros198756en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82255175377&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1429en_HK
dc.identifier.spage36en_HK
dc.identifier.epage42en_HK
dc.identifier.isiWOS:000300268200005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, Y=26643036800en_HK
dc.identifier.scopusauthoridZhuang, P=26032475900en_HK
dc.identifier.scopusauthoridShen, B=54783438700en_HK
dc.identifier.scopusauthoridZhang, Y=35217104300en_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.citeulike9946235-

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