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Article: A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield
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TitleA dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield
 
AuthorsSun, F1
Suen, PK1
Zhang, Y1
Liang, C1
Carrie, C2
Whelan, J2
Ward, JL3
Hawkins, ND3
Jiang, L4
Lim, BL1
 
Issue Date2012
 
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH
 
CitationNew Phytologist, 2012, v. 194 n. 1, p. 206-219 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x
 
Abstract* Overexpression of AtPAP2, a purple acid phosphatase (PAP) with a unique C-terminal hydrophobic motif in Arabidopsis, resulted in earlier bolting and a higher seed yield. Metabolite analysis showed that the shoots of AtPAP2 overexpression lines contained higher levels of sugars and tricarboxylic acid (TCA) metabolites. Enzyme assays showed that sucrose phosphate synthase (SPS) activity was significantly upregulated in the overexpression lines. The higher SPS activity arose from a higher level of SPS protein, and was independent of SnRK1. * AtPAP2 was found to be targeted to both plastids and mitochondria via its C-terminal hydrophobic motif. Ectopic expression of a truncated AtPAP2 without this C-terminal motif in Arabidopsis indicated that the subcellular localization of AtPAP2 is essential for its biological actions. * Plant PAPs are generally considered to mediate phosphorus acquisition and redistribution. AtPAP2 is the first PAP shown to modulate carbon metabolism and the first shown to be dual-targeted to both plastids and mitochondria by a C-terminal targeting signal. * One PAP-like sequence carrying a hydrophobic C-terminal motif could be identified in the genome of the smallest free-living photosynthetic eukaryote, Ostreococcus tauri. This might reflect a common ancestral function of AtPAP2-like sequences in the regulation of carbon metabolism.
 
ISSN0028-646X
2012 Impact Factor: 6.736
2012 SCImago Journal Rankings: 2.650
 
DOIhttp://dx.doi.org/10.1111/j.1469-8137.2011.04026.x
 
ISI Accession Number IDWOS:000300719400021
Funding AgencyGrant Number
HKSAR, ChinaHKU772710M
ITS158/09
Funding Information:

This project was supported by the General Research Fund (HKU772710M) and the Innovation and Technology Fund (ITS158/09) of the HKSAR, China. We thank Dr Yip's lab at HKU for kindly providing plant vectors and technical support for plant cultures. We thank Aimee Llewellyn and Delia Corol at Rothamsted Research for preparation of the analytical extracts and for 1H-NMR data collection, Dr David Secco at the University of Western Australia for his comments on this manuscript.

 
DC FieldValue
dc.contributor.authorSun, F
 
dc.contributor.authorSuen, PK
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorLiang, C
 
dc.contributor.authorCarrie, C
 
dc.contributor.authorWhelan, J
 
dc.contributor.authorWard, JL
 
dc.contributor.authorHawkins, ND
 
dc.contributor.authorJiang, L
 
dc.contributor.authorLim, BL
 
dc.date.accessioned2012-02-28T01:58:40Z
 
dc.date.available2012-02-28T01:58:40Z
 
dc.date.issued2012
 
dc.description.abstract* Overexpression of AtPAP2, a purple acid phosphatase (PAP) with a unique C-terminal hydrophobic motif in Arabidopsis, resulted in earlier bolting and a higher seed yield. Metabolite analysis showed that the shoots of AtPAP2 overexpression lines contained higher levels of sugars and tricarboxylic acid (TCA) metabolites. Enzyme assays showed that sucrose phosphate synthase (SPS) activity was significantly upregulated in the overexpression lines. The higher SPS activity arose from a higher level of SPS protein, and was independent of SnRK1. * AtPAP2 was found to be targeted to both plastids and mitochondria via its C-terminal hydrophobic motif. Ectopic expression of a truncated AtPAP2 without this C-terminal motif in Arabidopsis indicated that the subcellular localization of AtPAP2 is essential for its biological actions. * Plant PAPs are generally considered to mediate phosphorus acquisition and redistribution. AtPAP2 is the first PAP shown to modulate carbon metabolism and the first shown to be dual-targeted to both plastids and mitochondria by a C-terminal targeting signal. * One PAP-like sequence carrying a hydrophobic C-terminal motif could be identified in the genome of the smallest free-living photosynthetic eukaryote, Ostreococcus tauri. This might reflect a common ancestral function of AtPAP2-like sequences in the regulation of carbon metabolism.
 
dc.description.naturepreprint
 
dc.identifier.citationNew Phytologist, 2012, v. 194 n. 1, p. 206-219 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1469-8137.2011.04026.x
 
dc.identifier.eissn1469-8137
 
dc.identifier.epage219
 
dc.identifier.hkuros198745
 
dc.identifier.isiWOS:000300719400021
Funding AgencyGrant Number
HKSAR, ChinaHKU772710M
ITS158/09
Funding Information:

This project was supported by the General Research Fund (HKU772710M) and the Innovation and Technology Fund (ITS158/09) of the HKSAR, China. We thank Dr Yip's lab at HKU for kindly providing plant vectors and technical support for plant cultures. We thank Aimee Llewellyn and Delia Corol at Rothamsted Research for preparation of the analytical extracts and for 1H-NMR data collection, Dr David Secco at the University of Western Australia for his comments on this manuscript.

 
dc.identifier.issn0028-646X
2012 Impact Factor: 6.736
2012 SCImago Journal Rankings: 2.650
 
dc.identifier.issue1
 
dc.identifier.pmid22269069
 
dc.identifier.scopuseid_2-s2.0-84862777582
 
dc.identifier.spage206
 
dc.identifier.urihttp://hdl.handle.net/10722/145622
 
dc.identifier.volume194
 
dc.languageeng
 
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofNew Phytologist
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAcid Phosphatase - chemistry - metabolism
 
dc.subject.meshArabidopsis - drug effects - enzymology - growth and development
 
dc.subject.meshArabidopsis Proteins - chemistry - metabolism
 
dc.subject.meshGlycoproteins - chemistry - metabolism
 
dc.subject.meshSeeds - drug effects - growth and development
 
dc.titleA dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield
 
dc.typeArticle
 
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<contributor.author>Zhang, Y</contributor.author>
<contributor.author>Liang, C</contributor.author>
<contributor.author>Carrie, C</contributor.author>
<contributor.author>Whelan, J</contributor.author>
<contributor.author>Ward, JL</contributor.author>
<contributor.author>Hawkins, ND</contributor.author>
<contributor.author>Jiang, L</contributor.author>
<contributor.author>Lim, BL</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. University of Western Australia
  3. Rothamsted Research
  4. Chinese University of Hong Kong