Article: A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield
| Title | A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield | ||||
|---|---|---|---|---|---|
| Authors | Sun, F1 Suen, PK1 Zhang, Y1 Liang, C1 Carrie, C2 Whelan, J2 Ward, JL3 Hawkins, ND3 Jiang, L4 Lim, BL1 | ||||
| Issue Date | 2012 | ||||
| Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH | ||||
| Citation | New Phytologist, 2012, v. 194 n. 1, p. 206-219 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x | ||||
| Abstract | * Overexpression of AtPAP2, a purple acid phosphatase (PAP) with a unique C-terminal hydrophobic motif in Arabidopsis, resulted in earlier bolting and a higher seed yield. Metabolite analysis showed that the shoots of AtPAP2 overexpression lines contained higher levels of sugars and tricarboxylic acid (TCA) metabolites. Enzyme assays showed that sucrose phosphate synthase (SPS) activity was significantly upregulated in the overexpression lines. The higher SPS activity arose from a higher level of SPS protein, and was independent of SnRK1. * AtPAP2 was found to be targeted to both plastids and mitochondria via its C-terminal hydrophobic motif. Ectopic expression of a truncated AtPAP2 without this C-terminal motif in Arabidopsis indicated that the subcellular localization of AtPAP2 is essential for its biological actions. * Plant PAPs are generally considered to mediate phosphorus acquisition and redistribution. AtPAP2 is the first PAP shown to modulate carbon metabolism and the first shown to be dual-targeted to both plastids and mitochondria by a C-terminal targeting signal. * One PAP-like sequence carrying a hydrophobic C-terminal motif could be identified in the genome of the smallest free-living photosynthetic eukaryote, Ostreococcus tauri. This might reflect a common ancestral function of AtPAP2-like sequences in the regulation of carbon metabolism. | ||||
| ISSN | 0028-646X 2011 Impact Factor: 6.645 2011 SCImago Journal Rankings: 0.483 | ||||
| DOI | http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x | ||||
| ISI Accession Number ID | WOS:000300719400021
Funding Information: This project was supported by the General Research Fund (HKU772710M) and the Innovation and Technology Fund (ITS158/09) of the HKSAR, China. We thank Dr Yip's lab at HKU for kindly providing plant vectors and technical support for plant cultures. We thank Aimee Llewellyn and Delia Corol at Rothamsted Research for preparation of the analytical extracts and for 1H-NMR data collection, Dr David Secco at the University of Western Australia for his comments on this manuscript. |
| dc.contributor.author | Sun, F | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Suen, PK | ||||
| dc.contributor.author | Zhang, Y | ||||
| dc.contributor.author | Liang, C | ||||
| dc.contributor.author | Carrie, C | ||||
| dc.contributor.author | Whelan, J | ||||
| dc.contributor.author | Ward, JL | ||||
| dc.contributor.author | Hawkins, ND | ||||
| dc.contributor.author | Jiang, L | ||||
| dc.contributor.author | Lim, BL | ||||
| dc.date.accessioned | 2012-02-28T01:58:40Z | ||||
| dc.date.available | 2012-02-28T01:58:40Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | * Overexpression of AtPAP2, a purple acid phosphatase (PAP) with a unique C-terminal hydrophobic motif in Arabidopsis, resulted in earlier bolting and a higher seed yield. Metabolite analysis showed that the shoots of AtPAP2 overexpression lines contained higher levels of sugars and tricarboxylic acid (TCA) metabolites. Enzyme assays showed that sucrose phosphate synthase (SPS) activity was significantly upregulated in the overexpression lines. The higher SPS activity arose from a higher level of SPS protein, and was independent of SnRK1. * AtPAP2 was found to be targeted to both plastids and mitochondria via its C-terminal hydrophobic motif. Ectopic expression of a truncated AtPAP2 without this C-terminal motif in Arabidopsis indicated that the subcellular localization of AtPAP2 is essential for its biological actions. * Plant PAPs are generally considered to mediate phosphorus acquisition and redistribution. AtPAP2 is the first PAP shown to modulate carbon metabolism and the first shown to be dual-targeted to both plastids and mitochondria by a C-terminal targeting signal. * One PAP-like sequence carrying a hydrophobic C-terminal motif could be identified in the genome of the smallest free-living photosynthetic eukaryote, Ostreococcus tauri. This might reflect a common ancestral function of AtPAP2-like sequences in the regulation of carbon metabolism. | ||||
| dc.description.nature | preprint | ||||
| dc.identifier.citation | New Phytologist, 2012, v. 194 n. 1, p. 206-219 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x | ||||
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1469-8137.2011.04026.x | ||||
| dc.identifier.epage | 219 | ||||
| dc.identifier.hkuros | 198745 | ||||
| dc.identifier.isi | WOS:000300719400021
Funding Information: This project was supported by the General Research Fund (HKU772710M) and the Innovation and Technology Fund (ITS158/09) of the HKSAR, China. We thank Dr Yip's lab at HKU for kindly providing plant vectors and technical support for plant cultures. We thank Aimee Llewellyn and Delia Corol at Rothamsted Research for preparation of the analytical extracts and for 1H-NMR data collection, Dr David Secco at the University of Western Australia for his comments on this manuscript. | ||||
| dc.identifier.issn | 0028-646X 2011 Impact Factor: 6.645 2011 SCImago Journal Rankings: 0.483 | ||||
| dc.identifier.issue | 1 | ||||
| dc.identifier.pmid | 22269069 | ||||
| dc.identifier.scopus | eid_2-s2.0-84862777582 | ||||
| dc.identifier.spage | 206 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/145622 | ||||
| dc.identifier.volume | 194 | ||||
| dc.language | eng | ||||
| dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH | ||||
| dc.publisher.place | United Kingdom | ||||
| dc.relation.ispartof | New Phytologist | ||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||
| dc.subject.mesh | Acid Phosphatase - chemistry - metabolism | ||||
| dc.subject.mesh | Arabidopsis - drug effects - enzymology - growth and development | ||||
| dc.subject.mesh | Arabidopsis Proteins - chemistry - metabolism | ||||
| dc.subject.mesh | Glycoproteins - chemistry - metabolism | ||||
| dc.subject.mesh | Seeds - drug effects - growth and development | ||||
| dc.title | A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- University of Western Australia
- Rothamsted Research
- Chinese University of Hong Kong