Article: Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans
| Title | Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans | ||||
|---|---|---|---|---|---|
| Authors | Salvi, GE2 Aglietta, M2 Eick, S2 Sculean, A2 Lang, NP1 Ramseier, CA2 | ||||
| Issue Date | 2012 | ||||
| Publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR | ||||
| Citation | Clinical Oral Implants Research, 2012, v. 23 n. 2, p. 182-190 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1600-0501.2011.02220.x | ||||
| Abstract | OBJECTIVE: To monitor clinical, microbiological and host-derived alterations occurring around teeth and titanium implants during the development of experimental gingivitis/mucositis and their respective healing sequence in humans. MATERIAL AND METHODS: Fifteen subjects with healthy or treated periodontal conditions and restored with dental implants underwent an experimental 3-week period of undisturbed plaque accumulation in the mandible. Subsequently, a 3-week period with optimal plaque control was instituted. At Days 0, 7, 14, 21, 28, 35 and 42, the presence/absence of plaque deposits around teeth and implants was assessed, (plaque index [PlI]) and the gingival/mucosal conditions were evaluated (gingival index[GI]). Subgingival/submucosal plaque samples and gingival/mucosal crevicular fluid (CF) samples were collected from two pre-determined sites around each experimental unit. CF samples were analyzed for matrix-metalloproteinase-8 (MMP-8) and interleukin-1beta (IL-1beta). Microbial samples were analyzed using DNA-DNA hybridization for 40 species. RESULTS: During 3 weeks of plaque accumulation, the median PlI and GI increased significantly at implants and teeth. Implant sites yielded a greater increase in the median GI compared with tooth sites. Over the 6-week experimental period, the CF levels of MMP-8 were statistically significantly higher at implants compared with teeth (P<0.05). The CF IL-1beta levels did not differ statistically significantly between teeth and implants (P>0.05). No differences in the total DNA counts between implant and tooth sites were found at any time points. No differences in the detection frequency were found for putative periodontal pathogens between implant and tooth sites. CONCLUSION: Peri-implant soft tissues developed a stronger inflammatory response to experimental plaque accumulation when compared with that of their gingival counterparts. Experimental gingivitis and peri-implant mucositis were reversible at the biomarker level. Clinically, however, 3 weeks of resumed plaque control did not yield pre-experimental levels of gingival and peri-implant mucosal health indicating that longer healing periods are needed. | ||||
| ISSN | 0905-7161 2011 Impact Factor: 2.514 2011 SCImago Journal Rankings: 0.117 | ||||
| DOI | http://dx.doi.org/10.1111/j.1600-0501.2011.02220.x | ||||
| ISI Accession Number ID | WOS:000299098700007
Funding Information: This study was supported by the Swiss Society of Odontology (SSO), Grant No. 238-09. The competent laboratory assistance of Mrs Marianne Weibel and Mrs Regula Hirschi is highly appreciated. | ||||
| References | References in Scopus |
| dc.contributor.author | Salvi, GE | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Aglietta, M | ||||
| dc.contributor.author | Eick, S | ||||
| dc.contributor.author | Sculean, A | ||||
| dc.contributor.author | Lang, NP | ||||
| dc.contributor.author | Ramseier, CA | ||||
| dc.date.accessioned | 2012-02-28T01:52:58Z | ||||
| dc.date.available | 2012-02-28T01:52:58Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | OBJECTIVE: To monitor clinical, microbiological and host-derived alterations occurring around teeth and titanium implants during the development of experimental gingivitis/mucositis and their respective healing sequence in humans. MATERIAL AND METHODS: Fifteen subjects with healthy or treated periodontal conditions and restored with dental implants underwent an experimental 3-week period of undisturbed plaque accumulation in the mandible. Subsequently, a 3-week period with optimal plaque control was instituted. At Days 0, 7, 14, 21, 28, 35 and 42, the presence/absence of plaque deposits around teeth and implants was assessed, (plaque index [PlI]) and the gingival/mucosal conditions were evaluated (gingival index[GI]). Subgingival/submucosal plaque samples and gingival/mucosal crevicular fluid (CF) samples were collected from two pre-determined sites around each experimental unit. CF samples were analyzed for matrix-metalloproteinase-8 (MMP-8) and interleukin-1beta (IL-1beta). Microbial samples were analyzed using DNA-DNA hybridization for 40 species. RESULTS: During 3 weeks of plaque accumulation, the median PlI and GI increased significantly at implants and teeth. Implant sites yielded a greater increase in the median GI compared with tooth sites. Over the 6-week experimental period, the CF levels of MMP-8 were statistically significantly higher at implants compared with teeth (P<0.05). The CF IL-1beta levels did not differ statistically significantly between teeth and implants (P>0.05). No differences in the total DNA counts between implant and tooth sites were found at any time points. No differences in the detection frequency were found for putative periodontal pathogens between implant and tooth sites. CONCLUSION: Peri-implant soft tissues developed a stronger inflammatory response to experimental plaque accumulation when compared with that of their gingival counterparts. Experimental gingivitis and peri-implant mucositis were reversible at the biomarker level. Clinically, however, 3 weeks of resumed plaque control did not yield pre-experimental levels of gingival and peri-implant mucosal health indicating that longer healing periods are needed. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Clinical Oral Implants Research, 2012, v. 23 n. 2, p. 182-190 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1600-0501.2011.02220.x | ||||
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1600-0501.2011.02220.x | ||||
| dc.identifier.epage | 190 | ||||
| dc.identifier.hkuros | 198614 | ||||
| dc.identifier.isi | WOS:000299098700007
Funding Information: This study was supported by the Swiss Society of Odontology (SSO), Grant No. 238-09. The competent laboratory assistance of Mrs Marianne Weibel and Mrs Regula Hirschi is highly appreciated. | ||||
| dc.identifier.issn | 0905-7161 2011 Impact Factor: 2.514 2011 SCImago Journal Rankings: 0.117 | ||||
| dc.identifier.issue | 2 | ||||
| dc.identifier.pmid | 21806683 | ||||
| dc.identifier.scopus | eid_2-s2.0-84855933104 | ||||
| dc.identifier.spage | 182 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/145506 | ||||
| dc.identifier.volume | 23 | ||||
| dc.language | eng | ||||
| dc.publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | Clinical Oral Implants Research | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | The definitive version is available at www3.interscience.wiley.com | ||||
| dc.subject.mesh | Dental Implants | ||||
| dc.subject.mesh | Dental Plaque - complications - microbiology | ||||
| dc.subject.mesh | Gingivitis - etiology - microbiology | ||||
| dc.subject.mesh | Jaw, Edentulous, Partially - rehabilitation | ||||
| dc.subject.mesh | Mucositis - etiology - microbiology | ||||
| dc.title | Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans | ||||
| dc.type | Article |
Author Affiliations
- Prince Philip Dental Hospital
- Universität Bern