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Article: Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors

TitleAmmonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors
Authors
Keywords454 Pyrosequencing
Ammonia-oxidizing archaea
Ammonia-oxidizing bacteria
Municipal wastewater treatment plants
Issue Date2011
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2011, v. 91 n. 4, p. 1215-1225 How to Cite?
Abstract
In this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/145082
ISSN
2013 Impact Factor: 3.811
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
HKU
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study. Lin Ye wishes to thank HKU for the postgraduate studentship. We would also like to thank W. Chan and C. K. Wong for technical help in pyrosequencing.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorYe, Len_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorShao, MFen_HK
dc.contributor.authorLok, Sen_HK
dc.date.accessioned2012-02-21T05:44:04Z-
dc.date.available2012-02-21T05:44:04Z-
dc.date.issued2011en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2011, v. 91 n. 4, p. 1215-1225en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145082-
dc.description.abstractIn this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low. © 2011 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subject454 Pyrosequencingen_HK
dc.subjectAmmonia-oxidizing archaeaen_HK
dc.subjectAmmonia-oxidizing bacteriaen_HK
dc.subjectMunicipal wastewater treatment plantsen_HK
dc.titleAmmonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors&title=Applied Microbiology and Biotechnology&issn=01757598&date=2011-08-01&volume=91&issue=4& spage=1215&authors=Tong Zhang, Lin Ye, Amy Hin Yan Tong, <i>et al.</i>en_US
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00253-011-3408-yen_HK
dc.identifier.pmid21706171en_HK
dc.identifier.pmcidPMC3145087-
dc.identifier.scopuseid_2-s2.0-80052634538en_HK
dc.identifier.hkuros208080-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052634538&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1215en_HK
dc.identifier.epage1225en_HK
dc.identifier.eissn1432-0614en_US
dc.identifier.isiWOS:000293235300034-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridYe, L=36451639300en_HK
dc.identifier.scopusauthoridTong, AHY=7103351716en_HK
dc.identifier.scopusauthoridShao, MF=34868583400en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.citeulike9526831-

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