Article: Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors
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- Publisher Website: 10.1007/s00253-011-3408-y
- Scopus: eid_2-s2.0-80052634538
- PMID: 21706171
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| Title | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors |
|---|---|
| Authors | Zhang, T2 Ye, L2 Tong, AHY1 Shao, MF2 Lok, S1 |
| Keywords | 454 Pyrosequencing Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Municipal wastewater treatment plants |
| Issue Date | 2011 |
| Publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm |
| Citation | Applied Microbiology And Biotechnology, 2011, v. 91 n. 4, p. 1215-1225 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-011-3408-y |
| Abstract | In this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low. © 2011 The Author(s). |
| ISSN | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 |
| DOI | http://dx.doi.org/10.1007/s00253-011-3408-y |
| PubMed Central ID | PMC3145087 |
| References | References in Scopus |
| dc.contributor.author | Zhang, T | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Ye, L | ||||||
| dc.contributor.author | Tong, AHY | ||||||
| dc.contributor.author | Shao, MF | ||||||
| dc.contributor.author | Lok, S | ||||||
| dc.date.accessioned | 2012-02-21T05:44:04Z | ||||||
| dc.date.available | 2012-02-21T05:44:04Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | In this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low. © 2011 The Author(s). | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.description.other | Springer Open Choice, 21 Feb 2012 | ||||||
| dc.identifier.citation | Applied Microbiology And Biotechnology, 2011, v. 91 n. 4, p. 1215-1225 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-011-3408-y | ||||||
| dc.identifier.citeulike | 9526831 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s00253-011-3408-y | ||||||
| dc.identifier.eissn | 1432-0614 | ||||||
| dc.identifier.epage | 1225 | ||||||
| dc.identifier.hkuros | 208080 | ||||||
| dc.identifier.isi | WOS:000293235300034
Funding Information: The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study. Lin Ye wishes to thank HKU for the postgraduate studentship. We would also like to thank W. Chan and C. K. Wong for technical help in pyrosequencing. | ||||||
| dc.identifier.issn | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 | ||||||
| dc.identifier.issue | 4 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmcid | PMC3145087 | ||||||
| dc.identifier.pmid | 21706171 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80052634538 | ||||||
| dc.identifier.spage | 1215 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/145082 | ||||||
| dc.identifier.volume | 91 | ||||||
| dc.language | Eng | ||||||
| dc.publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | ||||||
| dc.publisher.place | Germany | ||||||
| dc.relation.ispartof | Applied Microbiology and Biotechnology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | The Author(s) | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject | 454 Pyrosequencing | ||||||
| dc.subject | Ammonia-oxidizing archaea | ||||||
| dc.subject | Ammonia-oxidizing bacteria | ||||||
| dc.subject | Municipal wastewater treatment plants | ||||||
| dc.title | Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The University of Hong Kong