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Article: Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China

TitleDiversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China
Authors
KeywordsAmmonia monooxygenase α-subunit (amoA) gene
Ammonia-oxidizing archaea (AOA)
Ammonia-oxidizing bacteria (AOB)
qPCR
T-RFLP
Issue Date2011
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2011, v. 90 n. 3, p. 1137-1145 How to Cite?
Abstract
The diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the sediment of the Pearl River Estuary were investigated by cloning and quantitative real-time polymerase chain reaction (qPCR). From one sediment sample S16, 36 AOA OTUs (3% cutoff) were obtained from three clone libraries constructed using three primer sets for amoA gene. Among the 36 OTUs, six were shared by all three clone libraries, two appeared in two clone libraries, and the other 28 were only recovered in one of the libraries. For AOB, only seven OTUs (based on 16S rRNA gene) and eight OTUs (based on amoA gene) were obtained, showing lower diversity than AOA. The qPCR results revealed that AOA amoA gene copy numbers ranged from 9.6×10 6 to 5.1×10 7 copies per gram of sediment and AOB amoA gene ranged from 9.5×10 4 to 6.2×10 5 copies per gram of sediment, indicating that the dominant ammonia-oxidizing microorganisms in the sediment of the Pearl River Estuary were AOA. The terminal restriction fragment length polymorphism results showed that the relative abundance of AOB species in the sediment samples of different salinity were significantly different, indicating that salinity might be a key factor shaping the AOB community composition. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/145057
ISSN
2013 Impact Factor: 3.811
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
HKU
CAS/SAFEA
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Lin Ye wish to thank HKU for the postgraduate studentship. This work was partially supported by the CAS/SAFEA International Partnership Program for Creative Research Teams to PY Qian.

References

 

DC FieldValueLanguage
dc.contributor.authorJin, Ten_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorYe, Len_HK
dc.contributor.authorLee, OOen_HK
dc.contributor.authorWong, YHen_HK
dc.contributor.authorQian, PYen_HK
dc.date.accessioned2012-02-21T05:44:35Z-
dc.date.available2012-02-21T05:44:35Z-
dc.date.issued2011en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2011, v. 90 n. 3, p. 1137-1145en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145057-
dc.description.abstractThe diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the sediment of the Pearl River Estuary were investigated by cloning and quantitative real-time polymerase chain reaction (qPCR). From one sediment sample S16, 36 AOA OTUs (3% cutoff) were obtained from three clone libraries constructed using three primer sets for amoA gene. Among the 36 OTUs, six were shared by all three clone libraries, two appeared in two clone libraries, and the other 28 were only recovered in one of the libraries. For AOB, only seven OTUs (based on 16S rRNA gene) and eight OTUs (based on amoA gene) were obtained, showing lower diversity than AOA. The qPCR results revealed that AOA amoA gene copy numbers ranged from 9.6×10 6 to 5.1×10 7 copies per gram of sediment and AOB amoA gene ranged from 9.5×10 4 to 6.2×10 5 copies per gram of sediment, indicating that the dominant ammonia-oxidizing microorganisms in the sediment of the Pearl River Estuary were AOA. The terminal restriction fragment length polymorphism results showed that the relative abundance of AOB species in the sediment samples of different salinity were significantly different, indicating that salinity might be a key factor shaping the AOB community composition. © 2011 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectAmmonia monooxygenase α-subunit (amoA) geneen_HK
dc.subjectAmmonia-oxidizing archaea (AOA)en_HK
dc.subjectAmmonia-oxidizing bacteria (AOB)en_HK
dc.subjectqPCRen_HK
dc.subjectT-RFLPen_HK
dc.subject.meshAmmonia - metabolism-
dc.subject.meshArchaea - classification - genetics - isolation and purification - metabolism-
dc.subject.meshBacteria - classification - genetics - isolation and purification - metabolism-
dc.subject.meshBiodiversity-
dc.subject.meshGeologic Sediments - microbiology-
dc.titleDiversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, Chinaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Diversity and quantity of ammonia-oxidizing <i>Archaea</i> and <i>Bacteria</i> in sediment of the Pearl River Estuary, China&title=Applied Microbiology and Biotechnology&issn=01757598&date=2011-05-01&volume=90&issue=3& spage=1137&authors=Tao Jin, Tong Zhang, Lin Ye, <i>et al.</i>en_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00253-011-3107-8en_HK
dc.identifier.pmid21286709en_HK
dc.identifier.pmcidPMC3076564-
dc.identifier.scopuseid_2-s2.0-79954661546en_HK
dc.identifier.hkuros192704-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79954661546&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1137en_HK
dc.identifier.epage1145en_HK
dc.identifier.eissn1432-0614en_US
dc.identifier.isiWOS:000289520000033-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridJin, T=46961330500en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridYe, L=36451639300en_HK
dc.identifier.scopusauthoridLee, OO=12791063700en_HK
dc.identifier.scopusauthoridWong, YH=36144724900en_HK
dc.identifier.scopusauthoridQian, PY=7102977224en_HK
dc.identifier.citeulike8784904-

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