Article: Soluble NgR fusion protein modulates the proliferation of neural progenitor cells via the notch pathway
| Title | Soluble NgR fusion protein modulates the proliferation of neural progenitor cells via the notch pathway | ||||||||||||||
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| Authors | Li, X1 2 Su, H1 Fu, QL2 Guo, J1 Lee, DHS4 So, KF1 3 Wu, W1 3 | ||||||||||||||
| Keywords | Myelin-associated glycoprotein Nogo-66 receptor NogoA Notch1 Rat neural progenitor cells | ||||||||||||||
| Issue Date | 2011 | ||||||||||||||
| Publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0364-3190 | ||||||||||||||
| Citation | Neurochemical Research, 2011, v. 36 n. 12, p. 2363-2372 [How to Cite?] DOI: http://dx.doi.org/10.1007/s11064-011-0562-7 | ||||||||||||||
| Abstract | NogoA, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein are CNS myelin molecules that bind to the neuronal Nogo-66 receptor (NgR) and inhibit axon growth. The NgR antagonist, soluble NgR1-Fc protein (sNgR-Fc), facilitates axon regeneration by neutralizing the inhibitory effects of myelin proteins in experimental models of CNS injury. Here we aim to investigate the effect of sNgR-Fc on the proliferation of neural progenitor cells (NPCs). The hippocampus cells of embryonic rats were isolated and cultured in vitro. The expression of nestin, βIII-Tubulin, GFAP and Nogo-A on these cells was observed using immunocytochemistry. In order to investigate the effect on proliferation of NPCs, sNgR-Fc, MAG-Fc chimera and Notch1 blocker were added respectively. The total cell number for the proliferated NPCs was counted. BrdU was applied and the rate of proliferating cells was examined. The level of Notch1 was analyzed using Western blotting. We identified that NogoA is expressed in NPCs. sNgR-Fc significantly enhanced the proliferation of NPCs in vitro as indicated by BrdU labeling and total cell count. This proliferation effect was abolished by the administration of MAG suggesting specificity. In addition, we demonstrate that sNgR-Fc is a potent activator for Notch1 and Notch1 antagonist reversed the effect of sNgR-Fc on NPC proliferation. Our results suggest that sNgR-Fc may modulate Nogo activity to induce NPC proliferation via the Notch pathway. © 2011 The Author(s). | ||||||||||||||
| ISSN | 0364-3190 2011 Impact Factor: 2.24 2011 SCImago Journal Rankings: 0.168 | ||||||||||||||
| DOI | http://dx.doi.org/10.1007/s11064-011-0562-7 | ||||||||||||||
| ISI Accession Number ID | WOS:000296516500021
Funding Information: This study was supported by funding from the Jessie Ho Professorship in Neuroscience, grants from the University of Hong Kong, National Basic Research Program of China (973Program, 2011CB707501), the Fundamental Research Funds for the Central Universities (21609101), NSFC (30801272, 81071030), Science and Technology Foundation of Guangdong Province, China (2010B031600089), and the Fundamental Research Funds for the Central Universities (09ykpy25, 09ykpy31). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Biogen Idec, Inc. provided the sNgR-Fc in the study. | ||||||||||||||
| PubMed Central ID | PMC3207133 | ||||||||||||||
| References | References in Scopus |
| dc.contributor.author | Li, X | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Su, H | ||||||||||||||
| dc.contributor.author | Fu, QL | ||||||||||||||
| dc.contributor.author | Guo, J | ||||||||||||||
| dc.contributor.author | Lee, DHS | ||||||||||||||
| dc.contributor.author | So, KF | ||||||||||||||
| dc.contributor.author | Wu, W | ||||||||||||||
| dc.date.accessioned | 2012-02-21T05:45:19Z | ||||||||||||||
| dc.date.available | 2012-02-21T05:45:19Z | ||||||||||||||
| dc.date.issued | 2011 | ||||||||||||||
| dc.description.abstract | NogoA, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein are CNS myelin molecules that bind to the neuronal Nogo-66 receptor (NgR) and inhibit axon growth. The NgR antagonist, soluble NgR1-Fc protein (sNgR-Fc), facilitates axon regeneration by neutralizing the inhibitory effects of myelin proteins in experimental models of CNS injury. Here we aim to investigate the effect of sNgR-Fc on the proliferation of neural progenitor cells (NPCs). The hippocampus cells of embryonic rats were isolated and cultured in vitro. The expression of nestin, βIII-Tubulin, GFAP and Nogo-A on these cells was observed using immunocytochemistry. In order to investigate the effect on proliferation of NPCs, sNgR-Fc, MAG-Fc chimera and Notch1 blocker were added respectively. The total cell number for the proliferated NPCs was counted. BrdU was applied and the rate of proliferating cells was examined. The level of Notch1 was analyzed using Western blotting. We identified that NogoA is expressed in NPCs. sNgR-Fc significantly enhanced the proliferation of NPCs in vitro as indicated by BrdU labeling and total cell count. This proliferation effect was abolished by the administration of MAG suggesting specificity. In addition, we demonstrate that sNgR-Fc is a potent activator for Notch1 and Notch1 antagonist reversed the effect of sNgR-Fc on NPC proliferation. Our results suggest that sNgR-Fc may modulate Nogo activity to induce NPC proliferation via the Notch pathway. © 2011 The Author(s). | ||||||||||||||
| dc.description.nature | published_or_final_version | ||||||||||||||
| dc.description.other | Springer Open Choice, 21 Feb 2012 | ||||||||||||||
| dc.identifier.citation | Neurochemical Research, 2011, v. 36 n. 12, p. 2363-2372 [How to Cite?] DOI: http://dx.doi.org/10.1007/s11064-011-0562-7 | ||||||||||||||
| dc.identifier.citeulike | 9686888 | ||||||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s11064-011-0562-7 | ||||||||||||||
| dc.identifier.eissn | 1573-6903 | ||||||||||||||
| dc.identifier.epage | 2372 | ||||||||||||||
| dc.identifier.hkuros | 197909 | ||||||||||||||
| dc.identifier.isi | WOS:000296516500021
Funding Information: This study was supported by funding from the Jessie Ho Professorship in Neuroscience, grants from the University of Hong Kong, National Basic Research Program of China (973Program, 2011CB707501), the Fundamental Research Funds for the Central Universities (21609101), NSFC (30801272, 81071030), Science and Technology Foundation of Guangdong Province, China (2010B031600089), and the Fundamental Research Funds for the Central Universities (09ykpy25, 09ykpy31). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Biogen Idec, Inc. provided the sNgR-Fc in the study. | ||||||||||||||
| dc.identifier.issn | 0364-3190 2011 Impact Factor: 2.24 2011 SCImago Journal Rankings: 0.168 | ||||||||||||||
| dc.identifier.issue | 12 | ||||||||||||||
| dc.identifier.pmcid | PMC3207133 | ||||||||||||||
| dc.identifier.pmid | 21822922 | ||||||||||||||
| dc.identifier.scopus | eid_2-s2.0-80755130385 | ||||||||||||||
| dc.identifier.spage | 2363 | ||||||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/145028 | ||||||||||||||
| dc.identifier.volume | 36 | ||||||||||||||
| dc.language | eng | ||||||||||||||
| dc.publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0364-3190 | ||||||||||||||
| dc.publisher.place | United States | ||||||||||||||
| dc.relation.ispartof | Neurochemical Research | ||||||||||||||
| dc.relation.references | References in Scopus | ||||||||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||||||||
| dc.rights | The original publication is available at www.springerlink.com | ||||||||||||||
| dc.rights | The Author(s) | ||||||||||||||
| dc.subject.mesh | Hippocampus - cytology | ||||||||||||||
| dc.subject.mesh | Myelin Proteins - biosynthesis - metabolism | ||||||||||||||
| dc.subject.mesh | Myelin-Associated Glycoprotein - pharmacology | ||||||||||||||
| dc.subject.mesh | Receptor, Notch1 - physiology | ||||||||||||||
| dc.subject.mesh | Recombinant Fusion Proteins - pharmacology | ||||||||||||||
| dc.subject | Myelin-associated glycoprotein | ||||||||||||||
| dc.subject | Nogo-66 receptor | ||||||||||||||
| dc.subject | NogoA | ||||||||||||||
| dc.subject | Notch1 | ||||||||||||||
| dc.subject | Rat neural progenitor cells | ||||||||||||||
| dc.title | Soluble NgR fusion protein modulates the proliferation of neural progenitor cells via the notch pathway | ||||||||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Sun Yat-Sen University
- Jinan University
- null