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Article: Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR

TitleCharacterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR
Authors
KeywordsAmmonia monooxygenase α-subunit (amoA) gene
Ammonia-oxidizing archaea (AOA) Ammonia-oxidizing bacteria (AOB)
QPCR
T-RFLP
Issue Date2010
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2010, v. 87 n. 3, p. 1167-1176 How to Cite?
AbstractUsing ammonia monooxygenase α-subunit (amoA) gene and 16S rRNA gene, the community structure and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in a nitrogen-removing reactor, which was operated for five phases, were characterized and quantified by cloning, terminal restriction fragment length polymorphism (T-RFLP), and quantitative polymerase chain reaction (qPCR). The results suggested that the dominant AOB in the reactor fell to the genus Nitrosomonas, while the dominant AOA belonged to Crenarchaeotal Group I.1a in phylum Crenarchaeota. Real-time PCR results demonstrated that the levels of AOB amoA varied from 2.9×10 3 to 2.3×10 5 copies per nanogram DNA, greatly (about 60 times) higher than those of AOA, which ranged from 1.7× 10 2 to 3.8× 10 3 copies per nanogram DNA. This indicated the possible leading role of AOB in the nitrification process in this study. T-RFLP results showed that the AOB community structure significantly shifted in different phases while AOA only showed one major peak for all the phases. The analyses also suggested that the AOB community was more sensitive than that of AOA to operational conditions, such as ammonia loading and dissolved oxygen. © The Author(s) 2010.
Persistent Identifierhttp://hdl.handle.net/10722/144983
ISSN
2014 Impact Factor: 3.337
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong General Research FundHKU7197/08E
HKU
Funding Information:

The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Qingmei Yan wish to thank HKU for the postgraduate studentship.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorJin, Ten_HK
dc.contributor.authorYan, Qen_HK
dc.date.accessioned2012-02-21T05:43:26Z-
dc.date.available2012-02-21T05:43:26Z-
dc.date.issued2010en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2010, v. 87 n. 3, p. 1167-1176en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144983-
dc.description.abstractUsing ammonia monooxygenase α-subunit (amoA) gene and 16S rRNA gene, the community structure and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in a nitrogen-removing reactor, which was operated for five phases, were characterized and quantified by cloning, terminal restriction fragment length polymorphism (T-RFLP), and quantitative polymerase chain reaction (qPCR). The results suggested that the dominant AOB in the reactor fell to the genus Nitrosomonas, while the dominant AOA belonged to Crenarchaeotal Group I.1a in phylum Crenarchaeota. Real-time PCR results demonstrated that the levels of AOB amoA varied from 2.9×10 3 to 2.3×10 5 copies per nanogram DNA, greatly (about 60 times) higher than those of AOA, which ranged from 1.7× 10 2 to 3.8× 10 3 copies per nanogram DNA. This indicated the possible leading role of AOB in the nitrification process in this study. T-RFLP results showed that the AOB community structure significantly shifted in different phases while AOA only showed one major peak for all the phases. The analyses also suggested that the AOB community was more sensitive than that of AOA to operational conditions, such as ammonia loading and dissolved oxygen. © The Author(s) 2010.en_HK
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectAmmonia monooxygenase α-subunit (amoA) geneen_HK
dc.subjectAmmonia-oxidizing archaea (AOA) Ammonia-oxidizing bacteria (AOB)en_HK
dc.subjectQPCRen_HK
dc.subjectT-RFLPen_HK
dc.subject.meshAmmonia - metabolism-
dc.subject.meshArchaea - classification - genetics - isolation and purification - metabolism-
dc.subject.meshBacteria - classification - genetics - isolation and purification - metabolism-
dc.subject.meshBioreactors - microbiology-
dc.subject.meshNitrogen - metabolism-
dc.subject.meshPolymorphism, Restriction Fragment Length-
dc.titleCharacterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCRen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR&title=Applied Microbiology and Biotechnology&issn=01757598&date=2010-07-01&volume=87&issue=3& spage=1167&authors=Tao Jin, Tong Zhang, Qingmei Yanen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00253-010-2595-2en_HK
dc.identifier.pmid20405121en_HK
dc.identifier.pmcidPMC2886134-
dc.identifier.scopuseid_2-s2.0-77955600012en_HK
dc.identifier.hkuros175526-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955600012&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1167en_HK
dc.identifier.epage1176en_HK
dc.identifier.eissn1432-0614en_US
dc.identifier.isiWOS:000278810200033-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridJin, T=46961330500en_HK
dc.identifier.scopusauthoridYan, Q=36055876900en_HK
dc.identifier.citeulike7080021-

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