Article: Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR
File Download
Links for fulltext
(May Require Subscription)
(May Require Subscription)
- Publisher Website: 10.1007/s00253-010-2595-2
- Scopus: eid_2-s2.0-77955600012
- PMID: 20405121
- Find via
Supplementary
-
Citations:
-
Appears in Collections:
| Title | Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Zhang, T1 Jin, T1 Yan, Q1 | ||||||
| Keywords | Ammonia monooxygenase α-subunit (amoA) gene Ammonia-oxidizing archaea (AOA) Ammonia-oxidizing bacteria (AOB) QPCR T-RFLP | ||||||
| Issue Date | 2010 | ||||||
| Publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | ||||||
| Citation | Applied Microbiology And Biotechnology, 2010, v. 87 n. 3, p. 1167-1176 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-010-2595-2 | ||||||
| Abstract | Using ammonia monooxygenase α-subunit (amoA) gene and 16S rRNA gene, the community structure and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in a nitrogen-removing reactor, which was operated for five phases, were characterized and quantified by cloning, terminal restriction fragment length polymorphism (T-RFLP), and quantitative polymerase chain reaction (qPCR). The results suggested that the dominant AOB in the reactor fell to the genus Nitrosomonas, while the dominant AOA belonged to Crenarchaeotal Group I.1a in phylum Crenarchaeota. Real-time PCR results demonstrated that the levels of AOB amoA varied from 2.9×10 3 to 2.3×10 5 copies per nanogram DNA, greatly (about 60 times) higher than those of AOA, which ranged from 1.7× 10 2 to 3.8× 10 3 copies per nanogram DNA. This indicated the possible leading role of AOB in the nitrification process in this study. T-RFLP results showed that the AOB community structure significantly shifted in different phases while AOA only showed one major peak for all the phases. The analyses also suggested that the AOB community was more sensitive than that of AOA to operational conditions, such as ammonia loading and dissolved oxygen. © The Author(s) 2010. | ||||||
| ISSN | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 | ||||||
| DOI | http://dx.doi.org/10.1007/s00253-010-2595-2 | ||||||
| ISI Accession Number ID | WOS:000278810200033
Funding Information: The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Qingmei Yan wish to thank HKU for the postgraduate studentship. | ||||||
| PubMed Central ID | PMC2886134 | ||||||
| References | References in Scopus |
| dc.contributor.author | Zhang, T | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Jin, T | ||||||
| dc.contributor.author | Yan, Q | ||||||
| dc.date.accessioned | 2012-02-21T05:43:26Z | ||||||
| dc.date.available | 2012-02-21T05:43:26Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | Using ammonia monooxygenase α-subunit (amoA) gene and 16S rRNA gene, the community structure and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in a nitrogen-removing reactor, which was operated for five phases, were characterized and quantified by cloning, terminal restriction fragment length polymorphism (T-RFLP), and quantitative polymerase chain reaction (qPCR). The results suggested that the dominant AOB in the reactor fell to the genus Nitrosomonas, while the dominant AOA belonged to Crenarchaeotal Group I.1a in phylum Crenarchaeota. Real-time PCR results demonstrated that the levels of AOB amoA varied from 2.9×10 3 to 2.3×10 5 copies per nanogram DNA, greatly (about 60 times) higher than those of AOA, which ranged from 1.7× 10 2 to 3.8× 10 3 copies per nanogram DNA. This indicated the possible leading role of AOB in the nitrification process in this study. T-RFLP results showed that the AOB community structure significantly shifted in different phases while AOA only showed one major peak for all the phases. The analyses also suggested that the AOB community was more sensitive than that of AOA to operational conditions, such as ammonia loading and dissolved oxygen. © The Author(s) 2010. | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.description.other | Springer Open Choice, 21 Feb 2012 | ||||||
| dc.identifier.citation | Applied Microbiology And Biotechnology, 2010, v. 87 n. 3, p. 1167-1176 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-010-2595-2 | ||||||
| dc.identifier.citeulike | 7080021 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s00253-010-2595-2 | ||||||
| dc.identifier.eissn | 1432-0614 | ||||||
| dc.identifier.epage | 1176 | ||||||
| dc.identifier.hkuros | 175526 | ||||||
| dc.identifier.isi | WOS:000278810200033
Funding Information: The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Qingmei Yan wish to thank HKU for the postgraduate studentship. | ||||||
| dc.identifier.issn | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 | ||||||
| dc.identifier.issue | 3 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmcid | PMC2886134 | ||||||
| dc.identifier.pmid | 20405121 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77955600012 | ||||||
| dc.identifier.spage | 1167 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/144983 | ||||||
| dc.identifier.volume | 87 | ||||||
| dc.language | Eng | ||||||
| dc.publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | ||||||
| dc.publisher.place | Germany | ||||||
| dc.relation.ispartof | Applied Microbiology and Biotechnology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | The Author(s) | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject.mesh | Ammonia - metabolism | ||||||
| dc.subject.mesh | Archaea - classification - genetics - isolation and purification - metabolism | ||||||
| dc.subject.mesh | Bacteria - classification - genetics - isolation and purification - metabolism | ||||||
| dc.subject.mesh | Bioreactors - microbiology | ||||||
| dc.subject.mesh | Nitrogen - metabolism | ||||||
| dc.subject.mesh | Polymorphism, Restriction Fragment Length | ||||||
| dc.subject | Ammonia monooxygenase α-subunit (amoA) gene | ||||||
| dc.subject | Ammonia-oxidizing archaea (AOA) Ammonia-oxidizing bacteria (AOB) | ||||||
| dc.subject | QPCR | ||||||
| dc.subject | T-RFLP | ||||||
| dc.title | Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong