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Article: Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
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TitleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
 
AuthorsLai, WF1
Tang, GP1 3
Wang, X4
Li, G4
Yao, H1 4
Shen, Z1 2
Lu, G4
Poon, WS4
Kung, HF4
Lin, MCM1 4
 
Keywordsβ-cyclodextrin
Nucleic acid delivery
Placenta mesenchymal stem cells
Poly(ethylenimine)
Tat peptide
 
Issue Date2011
 
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader
 
CitationBionanoscience, 2011, v. 1 n. 3, p. 89-96 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12668-011-0010-9
 
AbstractThis study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).
 
ISSN2191-1630
 
DOIhttp://dx.doi.org/10.1007/s12668-011-0010-9
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLai, WF
 
dc.contributor.authorTang, GP
 
dc.contributor.authorWang, X
 
dc.contributor.authorLi, G
 
dc.contributor.authorYao, H
 
dc.contributor.authorShen, Z
 
dc.contributor.authorLu, G
 
dc.contributor.authorPoon, WS
 
dc.contributor.authorKung, HF
 
dc.contributor.authorLin, MCM
 
dc.date.accessioned2012-02-21T05:43:12Z
 
dc.date.available2012-02-21T05:43:12Z
 
dc.date.issued2011
 
dc.description.abstractThis study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).
 
dc.description.naturepublished_or_final_version
 
dc.description.otherSpringer Open Choice, 21 Feb 2012
 
dc.identifier.citationBionanoscience, 2011, v. 1 n. 3, p. 89-96 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12668-011-0010-9
 
dc.identifier.doihttp://dx.doi.org/10.1007/s12668-011-0010-9
 
dc.identifier.eissn2191-1649
 
dc.identifier.epage96
 
dc.identifier.issn2191-1630
 
dc.identifier.issue3
 
dc.identifier.scopuseid_2-s2.0-84862575345
 
dc.identifier.spage89
 
dc.identifier.urihttp://hdl.handle.net/10722/144974
 
dc.identifier.volume1
 
dc.languageeng
 
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBioNanoScience
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.rightsThe original publication is available at www.springerlink.com
 
dc.rightsThe Author(s)
 
dc.subjectβ-cyclodextrin
 
dc.subjectNucleic acid delivery
 
dc.subjectPlacenta mesenchymal stem cells
 
dc.subjectPoly(ethylenimine)
 
dc.subjectTat peptide
 
dc.titleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
 
dc.typeArticle
 
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<contributor.author>Tang, GP</contributor.author>
<contributor.author>Wang, X</contributor.author>
<contributor.author>Li, G</contributor.author>
<contributor.author>Yao, H</contributor.author>
<contributor.author>Shen, Z</contributor.author>
<contributor.author>Lu, G</contributor.author>
<contributor.author>Poon, WS</contributor.author>
<contributor.author>Kung, HF</contributor.author>
<contributor.author>Lin, MCM</contributor.author>
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<description.abstract>This study aims to modify a cyclodextrin-PEI-based polymer, PEI-&#946;-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-&#946;-CyD and TAT peptide, the TAT-PEI-&#946;-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at &#948; 2.8-3.2 ppm), CyD (at &#948; 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at &#948; 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-&#946;-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6&#177;5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-&#946;-CyD, conjugation of the TAT peptide onto PEI-&#946;-CyD was demonstrated to improve the transfection efficiency of PEI-&#946;-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-&#946;-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-&#946;-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. &#169; 2011 The Author(s).</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiaotong University
  3. Zhejiang University
  4. Chinese University of Hong Kong