Article: Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells

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TitleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
AuthorsLai, WF1
Tang, GP1 3
Wang, X4
Li, G4
Yao, H1 4
Shen, Z1 2
Lu, G4
Poon, WS4
Kung, HF4
Lin, MCM1 4
Keywordsβ-cyclodextrin
Nucleic acid delivery
Placenta mesenchymal stem cells
Poly(ethylenimine)
Tat peptide
Issue Date2011
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader
CitationBionanoscience, 2011, v. 1 n. 3, p. 89-96 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12668-011-0010-9
AbstractThis study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).
ISSN2191-1630
DOIhttp://dx.doi.org/10.1007/s12668-011-0010-9
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLai, WF
dc.contributor.authorTang, GP
dc.contributor.authorWang, X
dc.contributor.authorLi, G
dc.contributor.authorYao, H
dc.contributor.authorShen, Z
dc.contributor.authorLu, G
dc.contributor.authorPoon, WS
dc.contributor.authorKung, HF
dc.contributor.authorLin, MCM
dc.date.accessioned2012-02-21T05:43:12Z
dc.date.available2012-02-21T05:43:12Z
dc.date.issued2011
dc.description.abstractThis study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).
dc.description.naturepublished_or_final_version
dc.description.otherSpringer Open Choice, 21 Feb 2012
dc.identifier.citationBionanoscience, 2011, v. 1 n. 3, p. 89-96 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s12668-011-0010-9
dc.identifier.doihttp://dx.doi.org/10.1007/s12668-011-0010-9
dc.identifier.eissn2191-1649
dc.identifier.epage96
dc.identifier.issn2191-1630
dc.identifier.issue3
dc.identifier.scopuseid_2-s2.0-84862575345
dc.identifier.spage89
dc.identifier.urihttp://hdl.handle.net/10722/144974
dc.identifier.volume1
dc.languageeng
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader
dc.publisher.placeUnited States
dc.relation.ispartofBioNanoScience
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.rightsThe original publication is available at www.springerlink.com
dc.rightsThe Author(s)
dc.subjectβ-cyclodextrin
dc.subjectNucleic acid delivery
dc.subjectPlacenta mesenchymal stem cells
dc.subjectPoly(ethylenimine)
dc.subjectTat peptide
dc.titleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiaotong University
  3. Zhejiang University
  4. Chinese University of Hong Kong