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Article: Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells

TitleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
Authors
Keywordsβ-cyclodextrin
Nucleic acid delivery
Placenta mesenchymal stem cells
Poly(ethylenimine)
Tat peptide
Issue Date2011
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader
Citation
Bionanoscience, 2011, v. 1 n. 3, p. 89-96 How to Cite?
Abstract
This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/144974
ISSN
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiaotong University
  3. Zhejiang University
  4. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLai, WFen_HK
dc.contributor.authorTang, GPen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorLi, Gen_HK
dc.contributor.authorYao, Hen_HK
dc.contributor.authorShen, Zen_HK
dc.contributor.authorLu, Gen_HK
dc.contributor.authorPoon, WSen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCMen_HK
dc.date.accessioned2012-02-21T05:43:12Z-
dc.date.available2012-02-21T05:43:12Z-
dc.date.issued2011en_HK
dc.identifier.citationBionanoscience, 2011, v. 1 n. 3, p. 89-96en_HK
dc.identifier.issn2191-1630en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144974-
dc.description.abstractThis study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3.2 ppm), CyD (at δ 5.2, 3.8-4.0 and 3.4-3. 6 ppm) and TAT (at δ 1.6-1.9 and 6.8-7.2 ppm) in the 1H NMR spectrum of TAT-PEI-β-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. © 2011 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/engineering/circuits+%26+systems/journal/12668?changeHeader en_HK
dc.relation.ispartofBioNanoScienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.rightsThe original publication is available at www.springerlink.comen_US
dc.rightsThe Author(s)en_US
dc.subjectβ-cyclodextrinen_HK
dc.subjectNucleic acid deliveryen_HK
dc.subjectPlacenta mesenchymal stem cellsen_HK
dc.subjectPoly(ethylenimine)en_HK
dc.subjectTat peptideen_HK
dc.titleCyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s12668-011-0010-9en_HK
dc.identifier.scopuseid_2-s2.0-84862575345en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862575345&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1en_HK
dc.identifier.issue3en_HK
dc.identifier.spage89en_HK
dc.identifier.epage96en_HK
dc.identifier.eissn2191-1649en_US
dc.publisher.placeUnited Statesen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridLai, WF=35603486100en_HK
dc.identifier.scopusauthoridTang, GP=7401634016en_HK
dc.identifier.scopusauthoridWang, X=54682502900en_HK
dc.identifier.scopusauthoridLi, G=55157745900en_HK
dc.identifier.scopusauthoridYao, H=13104506400en_HK
dc.identifier.scopusauthoridShen, Z=55265976200en_HK
dc.identifier.scopusauthoridLu, G=55042742900en_HK
dc.identifier.scopusauthoridPoon, WS=54933137900en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK

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