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Article: Development and organization of polarity-specific segregation of primary vestibular afferent fibers in mice

TitleDevelopment and organization of polarity-specific segregation of primary vestibular afferent fibers in mice
Authors
KeywordsCerebellum
Crista
Development
Hair cells
Macula
Mouse (CF1)
Issue Date2010
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 2010, v. 340 n. 2, p. 303-321 How to Cite?
AbstractA striking feature of vestibular hair cells is the polarized arrangement of their stereocilia as the basis for their directional sensitivity. In mammals, each of the vestibular end organs is characterized by a distinct distribution of these polarized cells. We utilized the technique of post-fixation transganglionic neuronal tracing with fluorescent lipid soluble dyes in embryonic and postnatal mice to investigate whether these polarity characteristics correlate with the pattern of connections between the endorgans and their central targets; the vestibular nuclei and cerebellum. We found that the cerebellar and brainstem projections develop independently from each other and have a non-overlapping distribution of neurons and afferents from E11.5 on. In addition, we show that the vestibular fibers projecting to the cerebellum originate preferentially from the lateral half of the utricular macula and the medial half of the saccular macula. In contrast, the brainstem vestibular afferents originate primarily from the medial half of the utricular macula and the lateral half of the saccular macula. This indicates that the line of hair cell polarity reversal within the striola region segregates almost mutually exclusive central projections. A possible interpretation of this feature is that this macular organization provides an inhibitory side-loop through the cerebellum to produce synergistic tuning effects in the vestibular nuclei. The canal cristae project to the brainstem vestibular nuclei and cerebellum, but the projection to the vestibulocerebellum originates preferentially from the superior half of each of the cristae. The reason for this pattern is not clear, but it may compensate for unequal activation of crista hair cells or may be an evolutionary atavism reflecting a different polarity organization in ancestral vertebrate ears. © 2010 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/144958
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Egyptian GovernmentGM-098
NASANAG 2-1353
NIH2R01 DC005590
Funding Information:

This work was supported by a grant from the Egyptian Government (GM-098; A. M.), by a NASA grant (NAG 2-1353; B.F. and A.M.), and by NIH grant (2R01 DC005590; B.F.).

References

 

DC FieldValueLanguage
dc.contributor.authorMaklad, Aen_HK
dc.contributor.authorKamel, Sen_HK
dc.contributor.authorWong, Een_HK
dc.contributor.authorFritzsch, Ben_HK
dc.date.accessioned2012-02-21T05:44:57Z-
dc.date.available2012-02-21T05:44:57Z-
dc.date.issued2010en_HK
dc.identifier.citationCell And Tissue Research, 2010, v. 340 n. 2, p. 303-321en_HK
dc.identifier.issn0302-766Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/144958-
dc.description.abstractA striking feature of vestibular hair cells is the polarized arrangement of their stereocilia as the basis for their directional sensitivity. In mammals, each of the vestibular end organs is characterized by a distinct distribution of these polarized cells. We utilized the technique of post-fixation transganglionic neuronal tracing with fluorescent lipid soluble dyes in embryonic and postnatal mice to investigate whether these polarity characteristics correlate with the pattern of connections between the endorgans and their central targets; the vestibular nuclei and cerebellum. We found that the cerebellar and brainstem projections develop independently from each other and have a non-overlapping distribution of neurons and afferents from E11.5 on. In addition, we show that the vestibular fibers projecting to the cerebellum originate preferentially from the lateral half of the utricular macula and the medial half of the saccular macula. In contrast, the brainstem vestibular afferents originate primarily from the medial half of the utricular macula and the lateral half of the saccular macula. This indicates that the line of hair cell polarity reversal within the striola region segregates almost mutually exclusive central projections. A possible interpretation of this feature is that this macular organization provides an inhibitory side-loop through the cerebellum to produce synergistic tuning effects in the vestibular nuclei. The canal cristae project to the brainstem vestibular nuclei and cerebellum, but the projection to the vestibulocerebellum originates preferentially from the superior half of each of the cristae. The reason for this pattern is not clear, but it may compensate for unequal activation of crista hair cells or may be an evolutionary atavism reflecting a different polarity organization in ancestral vertebrate ears. © 2010 Springer-Verlag.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_HK
dc.relation.ispartofCell and Tissue Researchen_HK
dc.rightsSpringer-Verlagen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectCerebellumen_HK
dc.subjectCristaen_HK
dc.subjectDevelopmenten_HK
dc.subjectHair cellsen_HK
dc.subjectMaculaen_HK
dc.subjectMouse (CF1)en_HK
dc.subject.meshAfferent Pathways - cytology - drug - effects - metabolism-
dc.subject.meshCell Polarity - drug effects-
dc.subject.meshVestibule, Labyrinth - cytology - drug effects - innervation-
dc.subject.meshBrain Stem - cytology - drug effects - metabolism-
dc.subject.meshEmbryo, Mammalian - cytology - drug effects - metabolism-
dc.titleDevelopment and organization of polarity-specific segregation of primary vestibular afferent fibers in miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Development and organization of polarity-specific segregation of primary vestibular afferent fibers in mice&title=Cell and Tissue Research&issn=0302766X&date=2010-05-01&volume=340&issue=2& spage=303&authors=Adel Maklad, Suzan Kamel, Elaine Wong, <i>et al.</i>en_US
dc.identifier.emailWong, E: elainewg@hku.hken_HK
dc.identifier.authorityWong, E=rp01718en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00441-010-0944-1en_HK
dc.identifier.pmid20424840-
dc.identifier.pmcidPMC2953634-
dc.identifier.scopuseid_2-s2.0-77953193899en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953193899&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume340en_HK
dc.identifier.issue2en_HK
dc.identifier.spage303en_HK
dc.identifier.epage321en_HK
dc.identifier.eissn1432-0878en_US
dc.identifier.isiWOS:000277136900008-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridMaklad, A=6602710454en_HK
dc.identifier.scopusauthoridKamel, S=36243589700en_HK
dc.identifier.scopusauthoridWong, E=36719382700en_HK
dc.identifier.scopusauthoridFritzsch, B=7006714975en_HK
dc.identifier.citeulike7115839-

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