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Article: Low-diversity fungal assemblage in an Antarctic Dry Valleys soil

TitleLow-diversity fungal assemblage in an Antarctic Dry Valleys soil
Authors
KeywordsAntarctica
Debaryomyces
Helicodendron
Soil fungi
Zalerion
Issue Date2012
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00300/index.htm
Citation
Polar Biology, 2012, v. 35 n. 4, p. 567-574 How to Cite?
AbstractThe McMurdo Dry Valleys of Antarctica present extreme environmental challenges. Life is restricted to patchy occurrence of lichens, mosses and invertebrates, plus soil microbial communities. Fungi have been described in lichen symbioses but relatively little is known about the occurrence of free-living soil fungi in the Dry Valleys. A challenge in estimating fungal species richness has been the extent to which estimates based on either cultivation or environmental DNA reflect the active assemblage in cold-arid soils. Here, we describe analysis for inland Dry Valleys soil of environmental DNA and RNA (cDNA) to infer total and putative metabolically active assemblages, respectively, plus cultivation approaches using a variety of laboratory growth conditions. Environmental sequences indicated a highly restricted assemblage of just seven phylotypes that affiliated phylogenetically within two known genera, Helicodendron and Zalerion, plus previously unidentified fungal phylotypes. None of the commonly encountered molds and mitosporic genera recorded from maritime Antarctic locations were encountered. A striking difference was observed in the frequency of recovery for phylotypes between libraries. This suggests that both species richness and beta diversity estimates based on DNA libraries have the potential to misinform putatively active assemblages. Cultivation yielded a cold-tolerant Zalerion strain that affiliated with DNA and RNA library clones, and a psychrotrophic yeast (Debaryomyces hansenii), which was not detected using either culture-independent approach. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/144937
ISSN
2015 Impact Factor: 1.711
2015 SCImago Journal Rankings: 0.983
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU7733/08 M
HKU7763/10
Antarctica New ZealandEvent K021B
Global Research Network for Fungal Biology
King Saud University
Funding Information:

The research was supported by the Hong Kong Research Grants Council (Grant numbers HKU7733/08 M, HKU7763/10). The authors wish to acknowledge the excellent logistic and field support provided by Antarctica New Zealand (Event K021B). K.D. Hyde thanks the Global Research Network for Fungal Biology and King Saud University for support.

References

 

DC FieldValueLanguage
dc.contributor.authorRao, Sen_HK
dc.contributor.authorChan, Yen_HK
dc.contributor.authorLacap, DCen_HK
dc.contributor.authorHyde, KDen_HK
dc.contributor.authorPointing, SBen_HK
dc.contributor.authorFarrell, RLen_HK
dc.date.accessioned2012-02-21T05:43:16Z-
dc.date.available2012-02-21T05:43:16Z-
dc.date.issued2012en_HK
dc.identifier.citationPolar Biology, 2012, v. 35 n. 4, p. 567-574en_HK
dc.identifier.issn0722-4060en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144937-
dc.description.abstractThe McMurdo Dry Valleys of Antarctica present extreme environmental challenges. Life is restricted to patchy occurrence of lichens, mosses and invertebrates, plus soil microbial communities. Fungi have been described in lichen symbioses but relatively little is known about the occurrence of free-living soil fungi in the Dry Valleys. A challenge in estimating fungal species richness has been the extent to which estimates based on either cultivation or environmental DNA reflect the active assemblage in cold-arid soils. Here, we describe analysis for inland Dry Valleys soil of environmental DNA and RNA (cDNA) to infer total and putative metabolically active assemblages, respectively, plus cultivation approaches using a variety of laboratory growth conditions. Environmental sequences indicated a highly restricted assemblage of just seven phylotypes that affiliated phylogenetically within two known genera, Helicodendron and Zalerion, plus previously unidentified fungal phylotypes. None of the commonly encountered molds and mitosporic genera recorded from maritime Antarctic locations were encountered. A striking difference was observed in the frequency of recovery for phylotypes between libraries. This suggests that both species richness and beta diversity estimates based on DNA libraries have the potential to misinform putatively active assemblages. Cultivation yielded a cold-tolerant Zalerion strain that affiliated with DNA and RNA library clones, and a psychrotrophic yeast (Debaryomyces hansenii), which was not detected using either culture-independent approach. © 2011 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00300/index.htmen_HK
dc.relation.ispartofPolar Biologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectAntarcticaen_HK
dc.subjectDebaryomycesen_HK
dc.subjectHelicodendronen_HK
dc.subjectSoil fungien_HK
dc.subjectZalerionen_HK
dc.titleLow-diversity fungal assemblage in an Antarctic Dry Valleys soilen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Low-diversity fungal assemblage in an Antarctic Dry Valleys soil&title=Polar Biology&issn=07224060&date=2011-09-25& spage=1&authors=Subramanya Rao, Yuki Chan, Donnabella C. Lacap, <i>et al.</i>en_US
dc.identifier.emailPointing, SB: pointing@hkucc.hku.hken_HK
dc.identifier.authorityPointing, SB=rp00771en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00300-011-1102-2en_HK
dc.identifier.scopuseid_2-s2.0-84858006401en_HK
dc.identifier.hkuros218930-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858006401&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue4en_HK
dc.identifier.spage567en_HK
dc.identifier.epage574en_HK
dc.identifier.eissn1432-2056en_US
dc.identifier.isiWOS:000301451600009-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridRao, S=7404179156en_HK
dc.identifier.scopusauthoridChan, Y=35725244600en_HK
dc.identifier.scopusauthoridLacap, DC=9640383000en_HK
dc.identifier.scopusauthoridHyde, KD=7102588111en_HK
dc.identifier.scopusauthoridPointing, SB=6603986412en_HK
dc.identifier.scopusauthoridFarrell, RL=35617552700en_HK
dc.identifier.citeulike9842925-

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